A Novel Defined PANoptosis-Related miRNA Signature for Predicting the Prognosis and Immune Characteristics in Clear Cell Renal Cell Carcinoma: A miRNA Signature for the Prognosis of ccRCC
Abstract
:1. Introduction
2. Results
2.1. Identification of Differentially Expressed miRNAs between Normal and Tumor Tissues
2.2. Development of the PANoptosis-Related Prognostic Signature Model
2.3. Assessment of the PANoptosis-Related Prognostic Signature Model
2.4. Clinical Characteristics of the Risk Score Model
2.5. The Relationship between Metabolic Process and Risk Groups
2.6. Characteristic Performance of the Tumor Microenvironment Cell Infiltration
2.7. Estimation of Immunotherapy and Chemotherapy Responses
2.8. Functional Enrichment Analysis of Distinctive miRNAs
3. Discussion
4. Materials and Methods
4.1. ccRCC Data Acquisition and Preprocessing
4.2. Identification of Differentially Expressed miRNAs
4.3. Establishment of Prognostic Risk Signature and Prediction Analysis
4.4. Clinical Characteristics of the Risk Score Model
4.5. Nomogram Construction and Evaluation
4.6. Pathway Enrichment Analysis
4.7. Estimation of Immune Cell Infiltration and Microenvironment Characterization
4.8. Cell Annotation and Single-Cell Trajectory Analysis
4.9. Prediction of Target Gene and Functional Analysis for miRNAs
4.10. Drug Sensitivity Analysis
4.11. Clinical Samples
4.12. Cell Culture
4.13. Cell Transfection
4.14. RNA Extraction and Real-Time Quantitative PCR
4.15. Cell Counting Kit-8 Assay
4.16. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristics | Variable | Total (n = 511) | Train Cohort (n = 256) | Test Cohort (n = 255) | Chi-Square (p) |
---|---|---|---|---|---|
Age | ≤65 | 338 (66.14%) | 170 (66.41%) | 168 (65.88%) | 0.9004 |
>65 | 173 (33.86%) | 86 (33.59%) | 87 (34.12%) | ||
Gender | Male | 332 (64.97%) | 169 (66.02%) | 163 (63.92%) | 0.6198 |
Female | 179 (35.03%) | 87 (33.98%) | 92 (36.08%) | ||
Grade | G1 | 13 (2.54%) | 8 (3.13%) | 5 (1.96%) | 0.202 |
G2 | 215 (42.07%) | 110 (42.97%) | 105 (41.18%) | ||
G3 | 201 (39.33%) | 95 (37.11%) | 106 (41.57%) | ||
G4 | 74 (14.48%) | 36 (14.06%) | 38 (14.9%) | ||
Unknown | 8 (1.57%) | 7 (2.73%) | 1 (0.39%) | ||
Pathologic stage | Stage I | 251 (49.12%) | 125 (48.83%) | 126 (49.41%) | 0.9046 |
Stage II | 55 (10.76%) | 29 (11.33%) | 26 (10.2%) | ||
Stage III | 123 (24.07%) | 59 (23.05%) | 64 (25.1%) | ||
Stage IV | 82 (16.05%) | 43 (16.8%) | 39 (15.29%) | ||
Tumor (T) | T1 | 257 (50.29%) | 128 (50%) | 129 (50.59%) | 0.8356 |
T2 | 67 (13.11%) | 34 (13.28%) | 33 (12.94%) | ||
T3 | 176 (34.44%) | 87 (33.98%) | 89 (34.9%) | ||
T4 | 11 (2.15%) | 7 (2.73%) | 4 (1.57%) | ||
Node (N) | N0 | 227 (44.42%) | 107 (41.8%) | 120 (47.06%) | 0.1988 |
N1 | 16 (3.13%) | 11 (4.3%) | 5 (1.96%) | ||
Unknown | 268 (52.45%) | 138 (53.91%) | 130 (50.98%) | ||
Metastasis (M) | M0 | 405 (79.26%) | 202 (78.91%) | 203 (79.61%) | 0.4726 |
M1 | 77 (15.07%) | 42 (16.41%) | 35 (13.73%) | ||
Unknown | 29 (5.68%) | 12 (4.69%) | 17 (6.67%) | ||
Survival status | Alive | 340 (66.54%) | 170 (66.41%) | 170 (66.67%) | 0.6576 |
Dead | 171 (33.46%) | 86 (33.59%) | 85 (33.33%) |
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Wang, Y.; Zhou, J.; Zhang, N.; Zhu, Y.; Zhong, Y.; Wang, Z.; Jin, H.; Wang, X. A Novel Defined PANoptosis-Related miRNA Signature for Predicting the Prognosis and Immune Characteristics in Clear Cell Renal Cell Carcinoma: A miRNA Signature for the Prognosis of ccRCC. Int. J. Mol. Sci. 2023, 24, 9392. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24119392
Wang Y, Zhou J, Zhang N, Zhu Y, Zhong Y, Wang Z, Jin H, Wang X. A Novel Defined PANoptosis-Related miRNA Signature for Predicting the Prognosis and Immune Characteristics in Clear Cell Renal Cell Carcinoma: A miRNA Signature for the Prognosis of ccRCC. International Journal of Molecular Sciences. 2023; 24(11):9392. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24119392
Chicago/Turabian StyleWang, Yanmei, Jia Zhou, Nan Zhang, Yiran Zhu, Yiming Zhong, Zhuo Wang, Hongchuan Jin, and Xian Wang. 2023. "A Novel Defined PANoptosis-Related miRNA Signature for Predicting the Prognosis and Immune Characteristics in Clear Cell Renal Cell Carcinoma: A miRNA Signature for the Prognosis of ccRCC" International Journal of Molecular Sciences 24, no. 11: 9392. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24119392