p53 Regulates Mitochondrial Dynamics in Vascular Smooth Muscle Cell Calcification
Abstract
:1. Introduction
2. Results
2.1. High Phosphate Treatment Alters Mitochondrial Dynamics in VSMCs
2.2. Regulators of Mitochondrial Fusion and Fission Drive Mitochondrial Elongation in Calcified VSMCs
2.3. Calcified VSMCs Show Increased Expression of Senescence Markers
2.4. p53 Suppression Blunts VSMC Calcification and Senescence
2.5. Knockdown of p53 Reverses Mitochondrial Dysfunction
3. Discussion
4. Materials and Methods
4.1. Maintenance of Mito-QC Mice
4.2. VSMC Isolation, Culture and Calcification
4.3. Transfection Assays
4.4. Cell Imaging
4.5. Senescence Associated β-Gal Staining
4.6. MitoTracker Staining
4.7. Measurement of ROS Levels
4.8. Mitophagy Assay
4.9. Mitochondrial Length Measurement
4.10. Analysis of Gene Expression
4.11. Immunoblotting
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Phadwal, K.; Tang, Q.-Y.; Luijten, I.; Zhao, J.-F.; Corcoran, B.; Semple, R.K.; Ganley, I.G.; MacRae, V.E. p53 Regulates Mitochondrial Dynamics in Vascular Smooth Muscle Cell Calcification. Int. J. Mol. Sci. 2023, 24, 1643. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24021643
Phadwal K, Tang Q-Y, Luijten I, Zhao J-F, Corcoran B, Semple RK, Ganley IG, MacRae VE. p53 Regulates Mitochondrial Dynamics in Vascular Smooth Muscle Cell Calcification. International Journal of Molecular Sciences. 2023; 24(2):1643. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24021643
Chicago/Turabian StylePhadwal, Kanchan, Qi-Yu Tang, Ineke Luijten, Jin-Feng Zhao, Brendan Corcoran, Robert K. Semple, Ian G. Ganley, and Vicky E. MacRae. 2023. "p53 Regulates Mitochondrial Dynamics in Vascular Smooth Muscle Cell Calcification" International Journal of Molecular Sciences 24, no. 2: 1643. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24021643