Role of Noradrenaline and Adrenoreceptors in Regulating Prostaglandin E2 Synthesis Cascade in Inflamed Endometrium of Pigs
Abstract
:1. Introduction
2. Results
2.1. The Effect of Noradrenaline and/or Antagonists of α1-, α2- and β-ARs on the PTGS-2 Protein Levels in the Endometrium
2.1.1. Noradrenaline Alone or Antagonists of α1-, α2- and β-ARs Alone
2.1.2. Noradrenaline Alone or Antagonists of α1-, α2- and β-ARs with Noradrenaline
2.2. The Effect of Noradrenaline and/or Antagonists of α1-, α2- and β-ARs on the mPTGES-1 Protein Levels in the Endometrium
2.2.1. Noradrenaline Alone or Antagonists of α1-, α2- and β-ARs Alone
2.2.2. Noradrenaline Alone or Antagonists of α1-, α2- and β-ARs with Noradrenaline
2.3. The Effect of Noradrenaline and/or Antagonists of α1-, α2- and β-ARs on the Secretion of PGE2 from the Endometrium
2.3.1. Noradrenaline Alone or Antagonists of α1-, α2- and β-ARs Alone
2.3.2. Noradrenaline Alone or Antagonists of α1-, α2- and β-ARs with Noradrenaline
3. Discussion
3.1. Our Findings in the Light of Available Literature
3.2. Clinical Significance of Findings
4. Materials and Methods
4.1. Animals
4.2. Study Design
4.3. Treatment of Endometrial Explants
4.4. Western Blot Method
4.5. ELISA Procedure
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Group | Protein Expression (Arbitrary Units) | |
---|---|---|---|
PTGS-2 | mPTGES-1 | ||
CV | CON | 0.2 ± 0.02 | 0.05 ± 0.01 |
E. coli | 0.41 ± 0.07 a,* | 0.07 ± 0.02 a | |
NA | CON | 0.32 ± 0.1 | 0.07 ± 0.01 |
E. coli | 0.94 ± 0.1 b,*** | 0.17 ± 0.01 b,*** | |
α1A anta. | CON | 0.18 ± 0.01 | 0.05 ± 0.01 |
E. coli | 0.23 ± 0.06 a | 0.09 ± 0.05 a | |
α1B anta. | CON | 0.32 ± 0.06 | 0.06 ± 0.01 |
E. coli | 0.48 ± 0.06 a | 0.09 ± 0.02 a | |
α1D anta. | CON | 0.22 ± 0.05 | 0.05 ± 0.01 |
E. coli | 0.24 ± 0.09 a | 0.08 ± 0.01a | |
α1ABD anta. | CON | 0.21 ± 0.04 | 0.04 ± 0.01 |
E. coli | 0.37 ± 0.05 a,* | 0.09 ± 0.02 a,* | |
α2A anta. | CON | 0.2 ± 0.04 | 0.06 ± 0.02 |
E. coli | 0.43 ± 0.04 a,* | 0.11 ± 0.02 a,b,* | |
α2B anta. | CON | 0.2 ± 0.03 | 0.06 ± 0.01 |
E. coli | 0.34 ± 0.06 a,* | 0.07 ± 0.03 a | |
α2C anta. | CON | 0.21 ± 0.03 | 0.07 ± 0.01 |
E. coli | 0.29 ± 0.05 a | 0.07 ± 0.02 a | |
α2ABC anta. | CON | 0.26 ± 0.03 | 0.07 ± 0.02 |
E. coli | 0.38 ± 0.04 a | 0.07 ± 0.02 a | |
β1 anta. | CON | 0.23 ± 0.06 | 0.04 ± 0.01 |
E. coli | 0.47 ± 0.07 a,* | 0.04 ± 0.01 a | |
β2 anta. | CON | 0.31 ± 0.03 | 0.06 ± 0.02 |
E. coli | 0.52 ± 0.07 a | 0.1 ± 0.02 a,b | |
β3 anta. | CON | 0.24 ± 0.05 | 0.05 ± 0.01 |
E. coli | 0.41 ± 0.06 a | 0.06 ± 0.03 a |
Treatment | Group | PGE2 (ng/g of Tissue) |
---|---|---|
CV | CON | 5.77 ± 0.8 a |
E. coli | 6.46 ± 0.71 a | |
NA | CON | 9.8 ± 0.45 b |
E. coli | 13.67 ± 0.6 b,** | |
α1A anta. | CON | 6.95 ± 0.6 a |
E. coli | 7.15 ± 0.71 a | |
α1B anta. | CON | 6.23 ± 0.65 a |
E. coli | 6.08 ± 0.74 a | |
α1D anta. | CON | 6.69 ± 0.77 a |
E. coli | 8.01 ± 0.92 a | |
α1ABD anta. | CON | 6.58 ± 0.63 a |
E. coli | 8.68 ± 0.74 a | |
α2A anta. | CON | 7.61 ± 0.92 a,b |
E. coli | 6.28 ± 0.54 a | |
α2B anta. | CON | 6.58 ± 0.8 a |
E. coli | 8.6 ± 0.61 a | |
α2C anta. | CON | 7.18 ± 0.74 a |
E. coli | 6.54 ± 0.55 a | |
α2ABC anta. | CON | 7.41±0.89 a |
E. coli | 8.32 ± 0.92 a | |
β1 anta. | CON | 6.17 ± 0.38 a |
E. coli | 6.74 ± 0.77 a | |
β2 anta. | CON | 5.57 ± 0.63 a |
E. coli | 6.1±0.65 a | |
β3 anta. | CON | 6.08 ± 0.69 a |
E. coli | 6.74 ± 0.78 a |
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Jana, B.; Całka, J.; Bulc, M.; Witek, K. Role of Noradrenaline and Adrenoreceptors in Regulating Prostaglandin E2 Synthesis Cascade in Inflamed Endometrium of Pigs. Int. J. Mol. Sci. 2023, 24, 5856. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24065856
Jana B, Całka J, Bulc M, Witek K. Role of Noradrenaline and Adrenoreceptors in Regulating Prostaglandin E2 Synthesis Cascade in Inflamed Endometrium of Pigs. International Journal of Molecular Sciences. 2023; 24(6):5856. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24065856
Chicago/Turabian StyleJana, Barbara, Jarosław Całka, Michał Bulc, and Krzysztof Witek. 2023. "Role of Noradrenaline and Adrenoreceptors in Regulating Prostaglandin E2 Synthesis Cascade in Inflamed Endometrium of Pigs" International Journal of Molecular Sciences 24, no. 6: 5856. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24065856