The Cellular Respiration of Endometrial Biopsies from Patients with Various Forms of Endometriosis
Abstract
:1. Introduction
2. Results
2.1. The OXPHOS Level in Endometrial Biopsies
2.2. Gene Expression of the Main Complexes, Involving in the OXPHOS and Glycolysis
3. Discussion
4. Materials and Methods
4.1. Experimental Design
4.2. Estimation of the Oxygen Consumption Rate by the Polarography
4.3. Estimation of the Relative mRNA Content by qRT-PCR
4.4. Statistical Analysis
5. Conclusions
Limitations of the Study
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer Sequence, Forward/Reverse (5′…3′) | Product Size, bp |
---|---|---|
CYC1 (Cytochrome c-1) | GAGGTGGAGGTTCAAGACGG/ TAGCTCGCACGATGTAGCTG | 160 |
COX4I1 (cytochrome c oxidase subunit IV isoform 1) | GCGGCAGAATGTTGGCTAC/ GATGAAGAACATGGCACCGC | 341 |
ATP5A1 (ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1) | TCAAAAGACTGGGACTGCTGA/ ATGTACGCGGGCAATACCAT | 127 |
HK1 (hexokinase 1) | AAGCAGACGCACAACAATGC/ AGGGCCAAGAAGTCACCATTC | 92 |
HK2 (hexokinase 2) | TGTGGATTCCAGCATTTGCC/ CCTGGCTTTGGTTTCATTGC | 70 |
PFKL (phosphofructokinase, liver type) | AAGGGTCAGGTGCAAGAAGTAG/ GATGCGGATGTTCTCCACAATG | 129 |
PFKM (phosphofructokinase, muscle type) | CAAAGATGTGACCAAGGCCATG/ TGCGAACCACTCTTAGATACCG | 138 |
PFKP (phosphofructokinase, platelet) | TGGACGGAGGCTCAAACATC/ CTGGAAGGAACACCCAGTCC | 519 |
PKM (pyruvate kinase M1/2) | GGAAGTGGGCAGCAAGATCT/ GCTCCATCCAGGACTGCATT | 564 |
H3F3A (H3 histone, family 3A) | AATCGACCGGTGGTAAAGCA/ GACGCTGGAAGGGAAGTTTG | 183 |
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Toniyan, K.A.; Malkov, A.A.; Biryukov, N.S.; Gorbacheva, E.Y.; Boyarintsev, V.V.; Ogneva, I.V. The Cellular Respiration of Endometrial Biopsies from Patients with Various Forms of Endometriosis. Int. J. Mol. Sci. 2024, 25, 3680. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms25073680
Toniyan KA, Malkov AA, Biryukov NS, Gorbacheva EY, Boyarintsev VV, Ogneva IV. The Cellular Respiration of Endometrial Biopsies from Patients with Various Forms of Endometriosis. International Journal of Molecular Sciences. 2024; 25(7):3680. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms25073680
Chicago/Turabian StyleToniyan, Konstantin A., Artyom A. Malkov, Nikolay S. Biryukov, Elena Yu. Gorbacheva, Valery V. Boyarintsev, and Irina V. Ogneva. 2024. "The Cellular Respiration of Endometrial Biopsies from Patients with Various Forms of Endometriosis" International Journal of Molecular Sciences 25, no. 7: 3680. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms25073680