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Article

Secretory Phospholipase A2-IIA Protein and mRNA Pools in Extracellular Vesicles of Bronchoalveolar Lavage Fluid from Patients with Early Acute Respiratory Distress Syndrome: A New Perception in the Dissemination of Inflammation?

1
Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 451 10 Ioannina, Greece
2
Section of Cell Biology & Biophysics, Department of Biology, School of Science, National and Kapodistrian University of Athens (NKUA), Panepistimioupolis, 15784 Athens, Greece
3
Laboratory of Pharmacology, School of Medicine, University of Ioannina, 451 10 Ioannina, Greece
4
Department of Intensive Care Medicine, School of Medicine, University of Ioannina, 451 10 Ioannina, Greece
5
Department of Materials Science, University of Ioannina, 451 10 Ioannina, Greece
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Pharmaceuticals 2020, 13(11), 415; https://0-doi-org.brum.beds.ac.uk/10.3390/ph13110415
Received: 6 November 2020 / Revised: 19 November 2020 / Accepted: 20 November 2020 / Published: 23 November 2020
(This article belongs to the Special Issue Lung Injury and Repair)
Secretory phospholipase-IIA A2 (sPLA2-IIA) is expressed in a variety of cell types under inflammatory conditions. Its presence in the bronchoalveolar lavage (BAL) fluid of patients with acute respiratory distress syndrome (ARDS) is associated with the severity of the injury. Exosomal type extracellular vesicles, (EVs), are recognized to perform intercellular communication. They may alter the immune status of recipient target cells through cargo shuttling. In this work, we characterized the exosomal type EVs isolated from BAL fluid of patients with early and late ARDS as compared to control/non-ARDS patients, through morphological (confocal and electron microscopy) and biochemical (dynamic light scattering, qRT-PCR, immunoblotting) approaches. We provide evidence for the presence of an sPLA2-IIA-carrying EV pool that coprecipitates with exosomes in the BAL fluid of patients with ARDS. PLA2G2A mRNA was present in all the samples, although more prominently expressed in early ARDS. However, the protein was found only in EVs from early phase ARDS. Under both forms, sPLA2-IIA might be involved in inflammatory responses of recipient lung cells during ARDS. The perception of the association of sPLA2-IIA to the early diagnosis of ARDS or even with a mechanism of development and propagation of lung inflammation can help in the adoption of appropriate and innovative therapeutic strategies. View Full-Text
Keywords: phospholipase A2; extracellular vesicles; exosomes; bronchoalveolar lavage fluid; acute lung injury; acute respiratory distress syndrome; immuno-TEM phospholipase A2; extracellular vesicles; exosomes; bronchoalveolar lavage fluid; acute lung injury; acute respiratory distress syndrome; immuno-TEM
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MDPI and ACS Style

Papadopoulos, S.; Kazepidou, E.; Antonelou, M.H.; Leondaritis, G.; Tsapinou, A.; Koulouras, V.P.; Avgeropoulos, A.; Nakos, G.; Lekka, M.E. Secretory Phospholipase A2-IIA Protein and mRNA Pools in Extracellular Vesicles of Bronchoalveolar Lavage Fluid from Patients with Early Acute Respiratory Distress Syndrome: A New Perception in the Dissemination of Inflammation? Pharmaceuticals 2020, 13, 415. https://0-doi-org.brum.beds.ac.uk/10.3390/ph13110415

AMA Style

Papadopoulos S, Kazepidou E, Antonelou MH, Leondaritis G, Tsapinou A, Koulouras VP, Avgeropoulos A, Nakos G, Lekka ME. Secretory Phospholipase A2-IIA Protein and mRNA Pools in Extracellular Vesicles of Bronchoalveolar Lavage Fluid from Patients with Early Acute Respiratory Distress Syndrome: A New Perception in the Dissemination of Inflammation? Pharmaceuticals. 2020; 13(11):415. https://0-doi-org.brum.beds.ac.uk/10.3390/ph13110415

Chicago/Turabian Style

Papadopoulos, Stylianos, Eleftheria Kazepidou, Marianna H. Antonelou, George Leondaritis, Alexia Tsapinou, Vasilios P. Koulouras, Apostolos Avgeropoulos, George Nakos, and Marilena E. Lekka 2020. "Secretory Phospholipase A2-IIA Protein and mRNA Pools in Extracellular Vesicles of Bronchoalveolar Lavage Fluid from Patients with Early Acute Respiratory Distress Syndrome: A New Perception in the Dissemination of Inflammation?" Pharmaceuticals 13, no. 11: 415. https://0-doi-org.brum.beds.ac.uk/10.3390/ph13110415

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