Serratia marcescens Outbreak at a Correctional Facility: Environmental Sampling, Laboratory Analyses and Genomic Characterization to Assess Sources and Persistence
Abstract
:1. Introduction
2. Materials and Methods
2.1. Environmental Sampling
2.2. Isolation, Culturing, and Identification of S. marcescens
2.3. Lab Experiment to Assess S. marcescens Survival in CB64
2.4. Whole Genome Sequencing
2.5. Genome Assembly, Annotation, and Relatedness
2.6. Antimicrobial Resistance (AMR) and Virulence Factors
2.7. Principal Component Analysis (PCA) of Virulence Factors
2.8. Construction of Trees
2.9. Epidemiological Investigation
3. Results
3.1. Detection of S. marcescens among Environmental Samples
3.2. S. marcescens Survival in CB64
3.3. Genome Assembly and Relatedness
3.4. Antimicrobial Resistance and Virulence Factors
3.5. Epidemiologic Investigation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Disclaimer
References
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Soln. No. | Soln. Description | Ave. Concentrations—Log10 CFU/mL | |||||
---|---|---|---|---|---|---|---|
Hours: | 0 | 1.5 | 3 | 24 | 48 | 168 (7 days) | |
1 | Undiluted CB64 | 0.0 | ND | 0.0 | 0.0 | 0.0 | 0.0 |
2 | 1:64 Unexpired CB64 (<24 h old) | 0.0 | ND | 0.0 | 0.0 | 0.0 | 0.0 |
3 | 1:64 Expired CB 64 (24 h old) | 0.0 | 0.0 | ND | 0.0 | 0.0 | 0.0 |
4 | 1:64 Expired CB64 (3 day old) | 0.0 | 0.0 | ND | 0.0 | 0.0 | 0.0 |
5 | 1:64 Expired CB64 (14 day old) | 0.0 | 0.0 | ND | 0.0 | 0.0 | 0.0 |
6 | 1:1000 Expired CB64 (24 h old) | 4.9 (2) * | 5.2 (3) | ND | 6.6 (9) | 7.2 (3) | 6.7 (6) |
7 | 1:1000 Expired CB64 (3 day old) | 5.1 (0) | 5.8 (13) | ND | 7.2 (7) | 7.4 (7) | 6.8 (20) |
8 | 1:1000 Expired CB64 (14 day old) | 4.4 (15) | 4.1 (14) | ND | 4.5 (23) | 6.3 (5) | 7.1 (4) |
9 | 1:10,000 Unexpired CB64 (<24 h old) | 5.0 (6) | ND | 2.3 (ND) | 3.8 (0) | 4.5 (14) | 7.8 (18) |
10 | 0.85% Saline (control) | 8.2 (ND) | ND | 7.4 (ND) | 8.7 (15) | 8.0 (10) | 8.0 (5) |
11 | Tap Water (control) | 7.7 (14) | 8.0 (5) | ND | ND | 7.7 (18) | 7.5 (10) |
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Ferguson, D.; Ryder, R.; Lunsford, R.; Dash, A.; Kamali, A.; Kimura, A.; Crandall, J.; Mukhopadhyay, R.; Dowless, H.; Ortiz, N.; et al. Serratia marcescens Outbreak at a Correctional Facility: Environmental Sampling, Laboratory Analyses and Genomic Characterization to Assess Sources and Persistence. Int. J. Environ. Res. Public Health 2023, 20, 6709. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph20176709
Ferguson D, Ryder R, Lunsford R, Dash A, Kamali A, Kimura A, Crandall J, Mukhopadhyay R, Dowless H, Ortiz N, et al. Serratia marcescens Outbreak at a Correctional Facility: Environmental Sampling, Laboratory Analyses and Genomic Characterization to Assess Sources and Persistence. International Journal of Environmental Research and Public Health. 2023; 20(17):6709. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph20176709
Chicago/Turabian StyleFerguson, Donna, Rahil Ryder, Rawni Lunsford, Arie Dash, Amanda Kamali, Akiko Kimura, John Crandall, Rituparna Mukhopadhyay, Heather Dowless, Nancy Ortiz, and et al. 2023. "Serratia marcescens Outbreak at a Correctional Facility: Environmental Sampling, Laboratory Analyses and Genomic Characterization to Assess Sources and Persistence" International Journal of Environmental Research and Public Health 20, no. 17: 6709. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph20176709