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Article

Amorphous Silica Nanoparticles Obtained by Laser Ablation Induce Inflammatory Response in Human Lung Fibroblasts

1
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
2
Department of Pharmacy, Faculty of Pharmacy, Titu Maiorescu University, 004051 Bucharest, Romania
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The National Institute for Research & Development in Chemistry and Petrochemistry (INCDCP-ICECHIM), 202 Splaiul Independentei, 060021 Bucharest, Romania
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Department of Preclinical Sciences, University of Agronomical Sciences and Veterinary Medicine, 105 Splaiul Independentei, 050097 Bucharest, Romania
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Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania
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Department of Histology, Faculty of Medicine, Vasile Goldis Western, University of Arad, 1 Feleacului, 310396 Arad, Romania
*
Author to whom correspondence should be addressed.
Received: 20 February 2019 / Revised: 22 March 2019 / Accepted: 25 March 2019 / Published: 28 March 2019
(This article belongs to the Special Issue Toxicity and Functionalization of Nanomaterials)
Silica nanoparticles (SiO2 NPs) represent environmentally born nanomaterials that are used in multiple biomedical applications. Our aim was to study the amorphous SiO2 NP-induced inflammatory response in MRC-5 human lung fibroblasts up to 72 hours of exposure. The intracellular distribution of SiO2 NPs was measured by transmission electron microscopy (TEM). The lactate dehydrogenase (LDH) test was used for cellular viability evaluation. We have also investigated the lysosomes formation, protein expression of interleukins (IL-1β, IL-2, IL-6, IL-8, and IL-18), COX-2, Nrf2, TNF-α, and nitric oxide (NO) production. Our results showed that the level of lysosomes increased in time after exposure to the SiO2 NPs. The expressions of interleukins and COX-2 were upregulated, whereas the expressions and activities of MMP-2 and MMP-9 decreased in a time-dependent manner. Our findings demonstrated that the exposure of MRC-5 cells to 62.5 µg/mL of SiO2 NPs induced an inflammatory response. View Full-Text
Keywords: silica nanoparticles; MRC-5 cell line; oxidative stress; inflammatory response silica nanoparticles; MRC-5 cell line; oxidative stress; inflammatory response
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MDPI and ACS Style

Voicu, S.N.; Balas, M.; Stan, M.S.; Trică, B.; Serban, A.I.; Stanca, L.; Hermenean, A.; Dinischiotu, A. Amorphous Silica Nanoparticles Obtained by Laser Ablation Induce Inflammatory Response in Human Lung Fibroblasts. Materials 2019, 12, 1026. https://0-doi-org.brum.beds.ac.uk/10.3390/ma12071026

AMA Style

Voicu SN, Balas M, Stan MS, Trică B, Serban AI, Stanca L, Hermenean A, Dinischiotu A. Amorphous Silica Nanoparticles Obtained by Laser Ablation Induce Inflammatory Response in Human Lung Fibroblasts. Materials. 2019; 12(7):1026. https://0-doi-org.brum.beds.ac.uk/10.3390/ma12071026

Chicago/Turabian Style

Voicu, Sorina N., Mihaela Balas, Miruna S. Stan, Bogdan Trică, Andreea I. Serban, Loredana Stanca, Anca Hermenean, and Anca Dinischiotu. 2019. "Amorphous Silica Nanoparticles Obtained by Laser Ablation Induce Inflammatory Response in Human Lung Fibroblasts" Materials 12, no. 7: 1026. https://0-doi-org.brum.beds.ac.uk/10.3390/ma12071026

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