Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial and Phage Strains
2.2. Transmission Electron Microscopy (TEM)
2.3. Phage Testing Procedures
2.4. Genome Analysis
2.5. Potential ARG Packaging
2.6. Data Analysis
2.7. Intact ARG Testing
3. Results
3.1. Phage Characterization
3.2. Genome Annotation and Characteristics
3.3. Potential ARG Packaging
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Size (bp) | HZA135 | HZA50 | HZP2 | HZ2R8 |
---|---|---|---|---|---|
blaTEM | 247(28.7%) | + | + | + | + |
752(87.3%) | + | + | − | − | |
qnrS | 428 (65.1%) | + | + | + | + |
619 (94.2%) | + | + | − | − | |
cmlA | 158 (12.5%) | + | + | + | + |
1188 (94.3%) | + | + | − | − | |
mcr-1 | 220 (13.5%) | + | + | + | + |
1495 (92.0%) | + | + | − | − | |
tetM | 171 (8.9%) | + | + | + | − |
1861 (96.9%) | + | − | − | − | |
sul3 | 128 (16.1%) | + | − | + | − |
714 (90.3%) | + | − | − | − | |
ermB | 189 (25.6%) | + | − | − | − |
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Liu, J.; Liu, P.; Feng, F.; Zhang, J.; Li, F.; Wang, M.; Sun, Y. Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction. Viruses 2020, 12, 1060. https://0-doi-org.brum.beds.ac.uk/10.3390/v12101060
Liu J, Liu P, Feng F, Zhang J, Li F, Wang M, Sun Y. Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction. Viruses. 2020; 12(10):1060. https://0-doi-org.brum.beds.ac.uk/10.3390/v12101060
Chicago/Turabian StyleLiu, Junlin, Peng Liu, Fenglin Feng, Junxuan Zhang, Fulin Li, Mianzhi Wang, and Yongxue Sun. 2020. "Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction" Viruses 12, no. 10: 1060. https://0-doi-org.brum.beds.ac.uk/10.3390/v12101060