Variations in BK Polyomavirus Immunodominant Large Tumor Antigen-Specific 9mer CD8 T-Cell Epitopes Predict Altered HLA-Presentation and Immune Failure
Abstract
:1. Introduction
2. Materials and Methods
2.1. Assessing Variation in the BKPyV LTag Sequence
2.2. BKPyV Genotypes, BKPyV-Variants and LTag-9mer Variants
2.3. Prediction of HLA-A and -B Binding of BKPyV Immunodominant LTag 9mer T-Cell Epitopes
2.4. BKPyV-Specific CD8 T-Cell Responses In Vitro
2.5. Statistical Analysis
3. Results
3.1. Identification of BKPyV LTag Variants
3.2. Identification of Amino Acid Exchanges in Immunodominant LTag 9mer T-Cell Epitopes
3.3. LTag-9mer Variants Affect HLA-B*07:02, HLA-B*08:01 and HLA-B*51:01 Binding
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Frequency of LTag 9mer Variants (%) | Number of LTag 9mer Variants | Number of LTag 9mer Variants with Changed HLA Binding 1 | |||
---|---|---|---|---|---|
↓ | ↑ | ↓↑ | Total | ||
<1% | 127 | 19 | 15 | 2 | 36 |
1–<5% | 14 | 4 | 1 | 1 | 6 |
5–<10% | 10 | 2 | 1 | - | 3 |
10–<25.0% | 8 | 2 | 1 | - | 3 |
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Leuzinger, K.; Kaur, A.; Wilhelm, M.; Hirsch, H.H. Variations in BK Polyomavirus Immunodominant Large Tumor Antigen-Specific 9mer CD8 T-Cell Epitopes Predict Altered HLA-Presentation and Immune Failure. Viruses 2020, 12, 1476. https://0-doi-org.brum.beds.ac.uk/10.3390/v12121476
Leuzinger K, Kaur A, Wilhelm M, Hirsch HH. Variations in BK Polyomavirus Immunodominant Large Tumor Antigen-Specific 9mer CD8 T-Cell Epitopes Predict Altered HLA-Presentation and Immune Failure. Viruses. 2020; 12(12):1476. https://0-doi-org.brum.beds.ac.uk/10.3390/v12121476
Chicago/Turabian StyleLeuzinger, Karoline, Amandeep Kaur, Maud Wilhelm, and Hans H. Hirsch. 2020. "Variations in BK Polyomavirus Immunodominant Large Tumor Antigen-Specific 9mer CD8 T-Cell Epitopes Predict Altered HLA-Presentation and Immune Failure" Viruses 12, no. 12: 1476. https://0-doi-org.brum.beds.ac.uk/10.3390/v12121476