Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye
Abstract
:1. Introduction
1.1. Increasing Complexity of In Vitro Models
1.2. Cell Types Used in In Vitro Models
1.3. In Vitro Models in Ophthalmic Research
2. Three-Dimensional In Vitro Models of Anterior Eye Tissues
2.1. Tear Film and Its Production Tissues
2.1.1. Lacrimal Gland
2.1.2. Meibomian Glands
2.2. Cornea
2.2.1. Corneal Epithelium 3D Models
2.2.2. Corneal Stroma 3D Models
2.2.3. Corneal Endothelium 3D Models
2.2.4. Multilayer or Whole Cornea Models (Corneal Equivalents)
2.3. Limbus
2.4. Conjunctiva
2.5. Conjunctiva and Uvea in Ocular Cancer
2.6. Trabecular Meshwork
2.7. Lens
3. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Layer | Milestones | References |
---|---|---|
Epithelium | Validation of reconstructed human cornea-like epithelium (RhCE) models for eye irritation tests | [53,54,55,56,57,58,59,60,61,62,63,64,65,66,67] |
Representation of corneal curvature | [72] | |
Stroma | Development of models with fibroblasts that produce their own extracellular matrix | [81,82,83,84,85,86] |
Use of 3D bioprinting to develop corneal equivalents | [88] | |
Endothelium | Combination of a stromal model with endothelial cells and the presence of Descemet’s membrane | [91] |
Fabrication of tissue-engineered endothelial grafts using decellularized corneal stroma as scaffolds | [92] | |
Corneal equivalents | Development of the first 3D model of the whole human cornea | [93] |
3D corneal model using human primary cells and the “self-assembly approach” (no use of exogenous material) | [94] | |
3D corneal model with epithelium, stroma, and innervation | [99,100,101,102] | |
Application of dynamic systems to a corneal 3D model | [104] | |
3D models of human corneal wound healing | [112,113] | |
Application of bioprinting to create corneal constructs | [114] |
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García-Posadas, L.; Diebold, Y. Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye. Pharmaceutics 2020, 12, 1215. https://0-doi-org.brum.beds.ac.uk/10.3390/pharmaceutics12121215
García-Posadas L, Diebold Y. Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye. Pharmaceutics. 2020; 12(12):1215. https://0-doi-org.brum.beds.ac.uk/10.3390/pharmaceutics12121215
Chicago/Turabian StyleGarcía-Posadas, Laura, and Yolanda Diebold. 2020. "Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye" Pharmaceutics 12, no. 12: 1215. https://0-doi-org.brum.beds.ac.uk/10.3390/pharmaceutics12121215