Early Detection of Bacterial Wilt in Tomato with Portable Hyperspectral Spectrometer
Round 1
Reviewer 1 Report
Although I consider that it is a very novel contribution to the remote sensing area, well written, and with a strong statistical analysis paper, there are some major concerns:
-The main concern that supports my description in not publishing this paper is that the complete experiments were carried out one time. You cannot draw conclusions based on only one main experiment. Consider that if we can not replicate the experiment in time (another year), we can compensate for this by replicating it in space (another location). This is relevant since the experiment was done under field conditions as mentioned in line 152.
The material and methods section lacks some information:
- The scale for wilting symptoms is not clear, is it a validated or published one? if so, then add the reference.
- The authors don’t mention the planting date, tomato growth stage, and days after planting (DAP) when measurements were done. This is relevant since the focus of the paper is on early detection
- I don’t see any measurement of the physiology of the plant, especially those related to water content or deficit, since the bacteria blockage the vascular system, at least to correlate to the hyperspectral data.
- Irrigation condition was not mentioned
- Inoculation method details were not mentioned
My recommendation is that, if this experiment can be replicates in another year or location, then can be published.
Comments for author File: 
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Author Response
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Author Response File: 
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Reviewer 2 Report
The manuscript titled “Early detection of bacterial wilt in tomato with portable hyperspectral spectrometer” by Cen et al. concerns an interesting topic, and it is acceptable a few minor revisions.
Introduction. Some important reviews should be mentioned to highlight the potential of hyperspectral data to assess biotic and abiotic stress in tree and crop species: Cotrozzi 2022. Spectroscopic detection of forest diseases: a review (1970–2020). J For Res 33, 21-38. https://0-doi-org.brum.beds.ac.uk/10.1007/s11676-021-01378-w; Mahlein et al. 2018. Hyperspectral Sensors and Imaging Technologies in Phytopathology: State of the Art. Annual Review of Phytopathology, 56:535-558. https://0-doi-org.brum.beds.ac.uk/10.1146/annurev-phyto-080417-050100; Cotrozzi et al. 2017. Reflectance spectroscopy: a novel approach to better understand and monitor the impact of air pollution on Mediterranean plants. Environ Sci Pollut Res. DOI 10.1007/s11356-017-9568-2
Bock CH, Poole GH, Parker PE, Gottwald TR (2010) Plant disease severity estimated visually, by digital photography and image analysis, and by hyperspectral imaging. Crit Rev Plant Sci 29: 59-107.
Kind regards,
Mahlein A-K (2015) Plant disease detection by imaging sensors – parallels and specific demands for precision agriculture and plant phenotyping. Plant Dis 100: 241-251.
M&M. More details about experimental design and material should be given (e.g. plant management, environmental conditions).
Author Response
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Author Response File: Author Response.docx
Reviewer 3 Report
In this study scientifically and significant results are presented. A novel model for early detection of tomato bacterial wilt disease is presented.
There are some technical mistakes in the manuscript - no word spacing could be seen in some paragraphs: Bacterial wilt(BW); detection[12,15], etc.
In the Introduction section – it would be better if the systematic names (Smith) Yabuuchi et al. are added to Ralstonia solanacearum, where the Latin name is mansioned for the first time in the text.
Author Response
- There are some technical mistakes in the manuscript - no word spacing could be seen in some paragraphs: Bacterial wilt(BW); detection[12,15], etc.
Response: We are sorry for these technical mistakes. We have double checked the full manuscript and fixed these mistakes.
- In the Introduction section – it would be better if the systematic names (Smith) Yabuuchi et al. are added to Ralstonia solanacearum, where the Latin name is mansioned for the first time in the text.
Response: Thank you for the comment. We have added the systematic names “(Smith) Yabuuchi et al.” in line 45, Page 1.
Reviewer 4 Report
The manuscript describes the spectrometric development to detect bacteria.
Here are my suggestions and questions:
1) Abstract
The abstract is extensive. Need to reduce.
2) The experimental and methods section is extensive. I understand the need to justify every detail of the procedure applied. However, I think some excerpts can be summarized. In addition, I suggest that authors transfer the Figure 4 to supplementary material.
3) Table 3
Transfer Table 3 for supplemental material.
Author Response
- Abstract：The abstract is extensive. Need to reduce.
Response: We agree with the comment. We have rewritten some redundant sentences and reduced the content in abstract. Please find the revisions in line 14-34, Page 1.
- The experimental and methods section is extensive. I understand the need to justify every detail of the procedure applied. However, I think some excerpts can be summarized. In addition, I suggest that authors transfer the Figure 4 to supplementary material.
Response: Thank you for the comment. We have reduced the content in the experimental and methods section. We have also transferred the Figure 4 to supplementary material Figure s1. In addition, we revised the figure numbers in manuscript due to the transfer of Figure 4 to supplementary material.
- Table 3, Transfer Table 3 for supplemental material.
Response: Thanks for your suggestion. It is a good idea to transfer Table 3 for supplemental material because at first we did not know how to deal with this problem, and you inspired us and provide us a good solution. We have transferred the Table 3 to supplementary material as Table s1.
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
