Next Article in Journal
Endophyte Infection and Methyl Jasmonate Treatment Increased the Resistance of Achnatherum sibiricum to Insect Herbivores Independently
Next Article in Special Issue
Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay to Detect Fusarium verticillioides in Poultry Feed Samples
Previous Article in Journal
Global Availability of Antivenoms: The Relevance of Public Manufacturing Laboratories
Previous Article in Special Issue
Carbon Quantum Dots Encapsulated Molecularly Imprinted Fluorescence Quenching Particles for Sensitive Detection of Zearalenone in Corn Sample
Article

Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit

by 1,†, 2,†, 1, 2 and 1,*
1
Institute of Environmental Health and Ecological Security, School of the Environment and Safety Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China
2
Institute of Plant Protection and Agro-Product Safety, Anhui Academy of Agricultural Sciences, Key Laboratory of Agro-Product Safety Risk Evaluation (Hefei), Ministry of Agriculture, South Road of agricultural science 40, Hefei 230031, China
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this paper.
Received: 12 November 2018 / Revised: 16 December 2018 / Accepted: 21 December 2018 / Published: 24 December 2018
(This article belongs to the Special Issue Advanced Methods for Mycotoxins Detection)
A total of 328 agricultural product samples highly suspected to be contaminated, from flour companies, feed companies, and livestock farms throughout China, were surveyed for deoxynivalenol (DON) contamination using a self-assembly enzyme-linked immunosorbent assay (ELISA) kit. An ELISA kit for DON was developed with a 4.9 ng mL−1 limit of detection (LOD) in working buffer and a 200 ng g−1 LOD in authentic samples. The DON contamination detection rate was 88.7%, concentrations ranged from 200.9 to 6480.6 ng g−1, and the highest DON contamination was found in distillers’ dried grains with solubles with an average of 3204.5 ng g−1. Wheat bran and wheat were found to be the most commonly contaminated samples, and the corn meal samples had the lowest average DON level. This ELISA kit is a powerful alternative method for the rapid, sensitive, specific, accurate, and high-throughput determination of DON and can meet the maximum requirement levels. This survey suggests that DON contamination in the Chinese market is serious, and the contamination risk deserves attention. Essential preventive measures should be implemented to ensure food safety and human health. View Full-Text
Keywords: deoxynivalenol; contamination; survey; ELISA kit; agricultural product deoxynivalenol; contamination; survey; ELISA kit; agricultural product
Show Figures

Graphical abstract

MDPI and ACS Style

Li, M.; Sun, M.; Hong, X.; Duan, J.; Du, D. Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit. Toxins 2019, 11, 6. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11010006

AMA Style

Li M, Sun M, Hong X, Duan J, Du D. Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit. Toxins. 2019; 11(1):6. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11010006

Chicago/Turabian Style

Li, Ming, Mingna Sun, Xia Hong, Jinsheng Duan, and Daolin Du. 2019. "Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit" Toxins 11, no. 1: 6. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11010006

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop