Extraction, Structure and Immunoregulatory Activity of Low Molecular Weight Polysaccharide from Dendrobium officinale
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Regents
2.2. Preparation of D. officinale Polysaccharide
2.3. Chemical Components Analysis of DOPs
2.4. Determination of Average Molecular Weight of DOPs
2.5. Determination of Monosaccharide Composition of DOPs
2.6. FT-IR Analysis of DOPs
2.7. NMR Analysis of DOPs
2.8. Animal Grouping and Experimental Design
2.9. Assays of Body Weights and Organs Indices
2.10. Assay of Splenic Lymphocyte Proliferation
2.11. Assay of Natural Killer (NK) Cells Activity
2.12. Assay of Macrophages Phagocytosis
2.13. Assay of Cytokines in Sera
2.14. Assays of Antioxidant Activities
2.15. Statistical Analysis
3. Results and Discussion
3.1. Purification, Chemical Constitutions, and Average Molecular Weight of DOPs
3.2. Monosaccharide Composition and FTIR Analysis
3.3. 1D 1H and 2D COSY Spectra Analysis
3.4. 2D HSQC and HMBC Spectra Analysis
3.5. Effects of DOPs on Organs Indices
3.6. Immune Cells Activities
3.7. Leukocytes Amounts and Cytokines Levels in Peripheral Blood
3.8. Antioxidant Activities In Vivo
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Monomers | H1/C1 ppm | H2/C2 ppm | H3/C3 ppm | H4/C4 ppm | H5/C5 ppm | H6,6′/C6 ppm |
---|---|---|---|---|---|---|
α-type Glc linkages | 5.41 102.63 | 3.61 74.42 | 3.72 75.54 | 3.66 79.72 | 3.97 76.23 | 3.86, 3.77 63.53 |
β-type Man linkages | 4.77 103.11 | 3.43 72.19 | 3.83 74.14 | 3.57 77.95 | 4.02 72.26 | 4.02, 3.74 67.23 |
Groups | TNF-α (ng/L) | IFN-γ (ng/L) | IL-2 (ng/L) |
---|---|---|---|
Blank group | 269.44 ± 13.81 | 303.67 ± 20.72 | 168.94 ± 9.68 |
Model group | 218.25 ± 14.05 α | 236.75 ± 14.84 α | 136.49 ± 9.83 α |
DOPs group (50 mg/kg) | 232.31 ± 17.12 β | 259.11 ± 15.37 β | 152.80 ± 8.76 β |
DOPs group (100 mg/kg) | 253.37 ± 12.75 β | 262.75 ± 13.77 β | 161.08 ± 11.13 β |
DOPs group (150 mg/kg) | 265.83 ± 14.01 β | 288.42 ± 19.37 β | 170.29 ± 13.82 β |
Groups | SOD (unit/mL) | GSH-Px (unit/mL) | MDA (nmol/mL) |
---|---|---|---|
Blank group | 235.92 ± 11.04 | 421.73 ± 21.36 | 3.98 ± 0.25 |
Model group | 186.53 ± 10.16 α | 351.16 ± 17.39 α | 5.99 ± 0.36 α |
DOPs group (50 mg/kg) | 203.41 ± 11.23 β | 375.98 ± 16.48 β | 5.53 ± 0.47 β |
DOPs group (100 mg/kg) | 216.33 ± 9.88 β | 402.42 ± 18.49 β | 4.83 ± 0.34 β |
DOPs group (150 mg/kg) | 236.52 ± 12.49 β | 427.58 ± 20.82 β | 4.07 ± 0.28 β |
Groups | SOD (Unit/mL) | GSH-Px (Unit/mL) | MDA (nmol/mL) |
---|---|---|---|
Blank group | 363.39 ± 17.69 | 836.75 ± 38.31 | 8.87 ± 0.42 |
Model group | 273.51 ± 16.98 α | 762.31 ± 37.87 α | 13.95 ± 0.78 α |
DOPs group (50 mg/kg) | 296.53 ± 16.29 β | 795.34 ± 36.92 β | 11.56 ± 0.69 β |
DOPs group (100 mg/kg) | 326.86 ± 14.96 β | 816.03 ± 39.11 β | 9.93 ± 0.51 β |
DOPs group (150 mg/kg) | 359.45 ± 15.48 β | 837.51 ± 42.67 β | 8.56 ± 0.40 β |
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Sun, S.-J.; Deng, P.; Peng, C.-E.; Ji, H.-Y.; Mao, L.-F.; Peng, L.-Z. Extraction, Structure and Immunoregulatory Activity of Low Molecular Weight Polysaccharide from Dendrobium officinale. Polymers 2022, 14, 2899. https://0-doi-org.brum.beds.ac.uk/10.3390/polym14142899
Sun S-J, Deng P, Peng C-E, Ji H-Y, Mao L-F, Peng L-Z. Extraction, Structure and Immunoregulatory Activity of Low Molecular Weight Polysaccharide from Dendrobium officinale. Polymers. 2022; 14(14):2899. https://0-doi-org.brum.beds.ac.uk/10.3390/polym14142899
Chicago/Turabian StyleSun, Su-Jun, Peng Deng, Chun-E Peng, Hai-Yu Ji, Long-Fei Mao, and Li-Zeng Peng. 2022. "Extraction, Structure and Immunoregulatory Activity of Low Molecular Weight Polysaccharide from Dendrobium officinale" Polymers 14, no. 14: 2899. https://0-doi-org.brum.beds.ac.uk/10.3390/polym14142899