Photocurable Thiol–yne Alginate Hydrogels for Regenerative Medicine Purposes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of Alginate yne
2.3. Hydrogel Preparation via Photo-Crosslinking
2.4. Hydrogel Characterization
2.5. Cell Viability and Proliferation
2.6. Statistical Analysis
3. Results and Discussion
3.1. Synthesis of SA–PA
3.2. Preparation and Characterization of Thiol–yne Hydrogels
3.3. Cell Viability and Proliferation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Crosslinker (HS-R-SH) | Molar Ratio (SA–PA:HS-R-SH) |
---|---|---|
0.05 DTT | DTT | 1:0.05 |
0.1 DTT | DTT | 1:0.1 |
0.2 DTT | DTT | 1:0.2 |
0.3 DTT | DTT | 1:0.3 |
0.05 S-PEG | PEG-SH | 1:0.05 |
0.1 S-PEG | PEG-SH | 1:0.1 |
0.2 S-PEG | PEG-SH | 1:0.2 |
0.3 S-PEG | PEG-SH | 1:0.3 |
Sample | Gel Point (s) | Induction Point (s) | Time to Plateau (s) | Storage Modulus G′ (KPa) | Yield Point (%) | Swelling Equilibrium (%) | Swelling Time to Plateau (h) | %GEL (%) |
---|---|---|---|---|---|---|---|---|
0.05 DTT | 237 | 153 | / | 0.03 | 1110 | 1045 ± 26 | 1 | 41 ± 6 |
0.1 DTT | 139 | 87 | / | 3.8 | 89 | 781 ± 63 | 1 | 72 ± 1 |
0.2 DTT | 153 | 97 | / | 0.81 | 337 | 835 ± 46 | 1 | 62 ± 0.5 |
0.3 DTT | 142 | 87 | / | 0.3 | 613 | 932 ± 16 | 1 | 53 ± 4 |
0.05 S-PEG | 129 | 100 | ≈200 | 0.11 | 570 | / | 1 | 33 ± 4 |
0.1 S-PEG | 100 | 86 | ≈200 | 1.7 | 185 | 802 ± 37 | 1 | 70 ± 3 |
0.2 S-PEG | 95 | 86 | ≈200 | 2.3 | 266 | 791 ± 47 | 1 | 75 ± 2 |
0.3 S-PEG | 98 | 86 | ≈200 | 1.1 | 377 | 1306 ± 222 | 1 | 57 ± 4 |
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Zanon, M.; Montalvillo-Jiménez, L.; Bosch, P.; Cue-López, R.; Martínez-Campos, E.; Sangermano, M.; Chiappone, A. Photocurable Thiol–yne Alginate Hydrogels for Regenerative Medicine Purposes. Polymers 2022, 14, 4709. https://0-doi-org.brum.beds.ac.uk/10.3390/polym14214709
Zanon M, Montalvillo-Jiménez L, Bosch P, Cue-López R, Martínez-Campos E, Sangermano M, Chiappone A. Photocurable Thiol–yne Alginate Hydrogels for Regenerative Medicine Purposes. Polymers. 2022; 14(21):4709. https://0-doi-org.brum.beds.ac.uk/10.3390/polym14214709
Chicago/Turabian StyleZanon, Michael, Laura Montalvillo-Jiménez, Paula Bosch, Raquel Cue-López, Enrique Martínez-Campos, Marco Sangermano, and Annalisa Chiappone. 2022. "Photocurable Thiol–yne Alginate Hydrogels for Regenerative Medicine Purposes" Polymers 14, no. 21: 4709. https://0-doi-org.brum.beds.ac.uk/10.3390/polym14214709