REVIWER

LINE

ANSWER

REVISOR 1

Over 50 % of used references were older than 10 years. Part "Matherials and methods" should be inproved, for exaple it is not clear how determination of lycpene was carried out?!

The oldest references were changed, it can be seen in the document in references

REVISOR 1

Only 1/3 references used in Discussion are issued in the past 5 years.

The changes in the older references were considered, it can be seen in the document in references

Line 91

Deleted parenthesis

Línea 96

Plastic mark included

REVISOR 2

Line 85: How does solarization affect microbes, p. Eg possible human pathogenic bacteria in manure? Many reports indicate the possible transfer of pathogens from manure in modified soil to vegetables.

In line 99 a sentence is attached enriching the effect of solarization on microorganisms

It is worth mentioning that solarized manure has the capacity that, when covered without  albedo plastics, the energy radiated by the sun generates temperatures between 64 and 74 ° C, causing pathogenic organisms such as fungi, bacteria, nematodes, weeds and insects to be killed and eliminated [17].

We can also justify that,

In aspects of food safety, and in organic crops, the manure that will be used goes through a sanitary certification where it shows the absence of food safety pathogens such as Klebsiella pnseuomiae, Salmonella spp, Campylobacter pp and Escherichia coli, Clostridium perfringens

A reference was added and consequently there was a numbering traversal

Salazar-Sosa E.; Fortis-Hernández, M.; López-Martínez, J.D.; Muños-Alvarado, C.L.; Lara-Macías, M.E.; Amado-Álvarez, J.P.; Vázquez-Vázquez,  C.; Trejo- Escareño, H.I.; Chavarría-Galicia, J.A. Comparison among solarization kind of pailes and their impact in the control of Cryptosporidium parvum and Giardia lamblia. International Research Journal of Agricultural Science and Soil Science. 2011. 1(9) 355-364.

REVISOR 2

Line 100: Add a source of chemical fertilizers

In line 109, a (source = reference) was added based on fertilization terms for this study

REVISOR 2

Line 107: Inoculation of tomato seeds with Azospirillum brasilense (Ab)

The title was changed

REVISOR 2

The variety could be described in the text.

On line 114 the following text was added:

In the present study, the variety TOP 2299 (Ahern Seeds®), type saladess, was evaluated; It is characterized by being a vigorous plant, strong bearing and with thick stems, medium internodes, with intermediate-late production cycles. Good development at high temperatures in spring-summer cycles. Fruits of large sizes, average 160 to 200 grams, maintain their size throughout the production cycle. In this phase, seeds were treated by means

REVISOR 2

Line 108: Tomato (Solanum lycopersicum, var aladette TOP 2299), check if correct, add botanical name and variety name.

Yes. It is correct

REVISOR 2

Line 108-112: Sentence too long, check it.

Paragraph was changed by:

In this phase, seeds were treated by means of a sterile distilled water wash to eliminate excess dyes and chemical products. Seeds were immersed in 70% alcohol for 10 sec and then carrying out three washes with sterile distilled water. Next, another disinfection with 2% sodium hypochlorite was carried out for 2 min, followed by washing with sterile distilled water according to [17].

REVISOR 2

Line 113:…. According to Lin et al. [17] (See entire manuscript for reference format)

121 changed

78 changed

Point  superíndice changed

155 changed

173

174

184 changed

221 changed

225 changed

249 changed

259 changed

318 super índice

438 eliminate “to”

443 deleted parenthesis

REVISOR 2

Check all references; some references are given incorrectly. For example: [17] should be like:

Lin TF, Huang HI, Shen FT, Young CC. Los protones del ácido glucónico son el principal factor responsable de la disolución del fosfato tricálcico por Burkholderia cepacia CC-Al74. Bioresour Technol. Mayo de 2006; 97 (7): 957-60. doi: 10.1016 / j.biortech.2005.02.017. Publicación electrónica del 13 de diciembre de 2005 PMID: 16356711.

no Ta-Fa et al.

Correct thanks!!

REVISOR 2

Line 114: According to [18], add the name

Made online 123 !!!

REVISOR 2

Line 115: Why only Clavibacter michiganensis ?, 2% sodium hypochlorite used for all types of microbes?

Clavibacter is a seed pathogen that any producer seeks to eliminate… .. extremely aggressive !!! …… it is possible that with this treatment some endophytic bacteria are also eliminated but it has not been reported !!

REVISOR 2

Lines 120-125, sentence too long, check it. Also describe the procedure for bacterial growth in the medium.

Línea 130-135 was changed

Then, seeds were placed in growth chambers containing fine sand and seed covered with a fine cap of peat-moss (Sunshine, Sun I Cry Horticulture Canada, Ltd.) at 25 ± 3 °C in dark conditions of 24 h at H.r. of 45. Once seeds emergence were located under greenhouse conditions (36° C, H.r. of 40%, and 3000 luxe) during 45 days. Before transplanting, seedlings were inoculated at 14 and 26 days after sowed (das), immersing the root system in a bacterial base solution of Azospirillum brasilense with 1 X 10⁸ CFU^.mL during 10 s.

Line 126: what does "for 10 s" mean

Seconds

Changed by 10”

REVISOR 2

The growing condition of the field side plants should be described in detail, temperature, lights, sowing, duration of growth, climatic irrigation, etc.

Lline 169 and table 2 was considered

The conditions (T° C, H.r., photsynthetically active radiation (Rfa: me^. M^-2 s^-1) and annual mean insolation (Ima^.h), on the shadow house conditions are indicate in Table 2.

REVISOR 2

Line 145: "according to [20], and fungi, according to [21]". Please see the full MS to see the reference format (INGREDIENTS)

Ready!!! Thanks

REVISOR 2

Line 148: How have you identified pathogenic fungi, molecular biology tools? Describe in detail. Why were other biological properties of the soil not measured? Microbial diversity, soil enzymes, etc.

In this study we only considered that in the transplant soil there were no plant pathogens ……… the tests were biochemical and taxonomic keys.

REVISOR 2

Line 169: 2.8. Treatments and experimental design ”This part should move to the top where the treatments were described.

To be more explicit, the same section was left

REVISOR 2

Line 181: "was calculated [22]:" okay? Check the citation format.

Your previous observation was considered

REVISOR 2

Line 184: “o the arc-sin” is not clear.

216 line

The sentence was restructured

REVISOR 2

Line 230: what does quantification of bacteria mean? The microbial diversity of the rhizosphere? Or the survival of inoculated bacteria. Clarify and review this part. Why were the samples not taken during the growing season, but only at harvest? All these measures should also be described in the study objective.

It refers to the fact that at the end of the experiment they wanted to corroborate the presence of Azospirillum

No other samplings were made, since inoculations were made in seed, in seedling and 60 and 90 days after germination

Line 130-169

REVISOR 2

Lines 312-316: This part is not clear. How have you distinguished inoculated bacteria from indigenous soil bacteria? Or was the total microbial count described?

It refers to the fact that at the end of the experiment WE wanted to corroborate the presence of Azospirillum

REVISOR 2

Lines 418-419: the results part should not be repeated. Describe in the methods section how the bacteria inoculated after the plant growth stage were determined.

Eliminated the two lines

Line 454

REVISOR 3

line 17 and 18. I don't see the need for this sentence in the abstract as it is not directly related to the topic. Also, this scientific approach could be used outside of Mexico or the region in Mexico where the research was conducted.

The sentence was restructured but without eliminating the sentence, to enhance and sequence the importance of the tomato from Mexico worldwide

have probiotic properties and based on this, since this spring-2020 summer season, Mexico

REVISOR 3

Line 101. The nutritional content of the fertilizer could be expressed in terms of percentage N, P, and K.

it is not common to express it in%.

The NPK (or N-P-K) index is the acronym for the relationship between the chemical elements Nitrogen (N), Phosphorus (P) and Potassium (K) that are commonly used in fertilizers.

REVISOR 3

paragraph 108. There needs to be an introduction to the use of A. brasilense for crops. How long has it been used? For what crops? What is the mode of action? How has it been productive? In other words, there needs to be a comprehensive paragraph describing the use of A. brasilense to justify this study.

58-60 was considered

REVISOR 3

Line 173. How many tomato plants per replicate? Or 1 plant constituted 1 of the 14 replicates? Please explain.

Line 200-2003 paragraph reestrcutured

An experimental design of random blocks with fourteen repetitions for each variable were considered under a factorial design (2 X 6), where factor A corresponds to the inoculum with Ab, while factor B corresponds to solarized manure; 504 experimental units evaluated were all the blocks.

REVISOR 3

Paragraph 246. Please explain the importance of seedling growth rate with first few days of emergence. How is this relevant

Is the same (in parentehsis was wrote emergence rate)

Line 284

Results according to the proposed methodology show that in the emergency variable (emergence rate), seed inoculated with Azospirillum brasilense (Ab)……..

REVISOR 3

Line 467. I think reference to SARs is not relevant to this study and should be removed.

We request that it be added to agree with the introduction of the importance of consuming probiotic horticultural products such as tomato, properties that strengthen the body and more in this international biosecurity contingency

which is desirable in these days when the world population demands to be fed with plant products rich in biomolecules that mitigate the aggressiveness of a causal agent such as SARS-Covid-19; important properties that, due to their health effects, suggest that they prevent the risks of acquiring chronic diseases such as cancer and cardiovascular diseases [69].

REVIWER

LINE

ANSWER

REVISOR 1

Over 50 % of used references were older than 10 years. Part "Matherials and methods" should be inproved, for exaple it is not clear how determination of lycpene was carried out?!

The oldest references were changed, it can be seen in the document in references

REVISOR 1

Only 1/3 references used in Discussion are issued in the past 5 years.

The changes in the older references were considered, it can be seen in the document in references

Line 91

Deleted parenthesis

Línea 96

Plastic mark included

REVISOR 2

Line 85: How does solarization affect microbes, p. Eg possible human pathogenic bacteria in manure? Many reports indicate the possible transfer of pathogens from manure in modified soil to vegetables.

In line 99 a sentence is attached enriching the effect of solarization on microorganisms

It is worth mentioning that solarized manure has the capacity that, when covered without  albedo plastics, the energy radiated by the sun generates temperatures between 64 and 74 ° C, causing pathogenic organisms such as fungi, bacteria, nematodes, weeds and insects to be killed and eliminated [17].

We can also justify that,

In aspects of food safety, and in organic crops, the manure that will be used goes through a sanitary certification where it shows the absence of food safety pathogens such as Klebsiella pnseuomiae, Salmonella spp, Campylobacter pp and Escherichia coli, Clostridium perfringens

A reference was added and consequently there was a numbering traversal

Salazar-Sosa E.; Fortis-Hernández, M.; López-Martínez, J.D.; Muños-Alvarado, C.L.; Lara-Macías, M.E.; Amado-Álvarez, J.P.; Vázquez-Vázquez,  C.; Trejo- Escareño, H.I.; Chavarría-Galicia, J.A. Comparison among solarization kind of pailes and their impact in the control of Cryptosporidium parvum and Giardia lamblia. International Research Journal of Agricultural Science and Soil Science. 2011. 1(9) 355-364.

REVISOR 2

Line 100: Add a source of chemical fertilizers

In line 109, a (source = reference) was added based on fertilization terms for this study

REVISOR 2

Line 107: Inoculation of tomato seeds with Azospirillum brasilense (Ab)

The title was changed

REVISOR 2

The variety could be described in the text.

On line 114 the following text was added:

In the present study, the variety TOP 2299 (Ahern Seeds®), type saladess, was evaluated; It is characterized by being a vigorous plant, strong bearing and with thick stems, medium internodes, with intermediate-late production cycles. Good development at high temperatures in spring-summer cycles. Fruits of large sizes, average 160 to 200 grams, maintain their size throughout the production cycle. In this phase, seeds were treated by means

REVISOR 2

Line 108: Tomato (Solanum lycopersicum, var aladette TOP 2299), check if correct, add botanical name and variety name.

Yes. It is correct

REVISOR 2

Line 108-112: Sentence too long, check it.

Paragraph was changed by:

In this phase, seeds were treated by means of a sterile distilled water wash to eliminate excess dyes and chemical products. Seeds were immersed in 70% alcohol for 10 sec and then carrying out three washes with sterile distilled water. Next, another disinfection with 2% sodium hypochlorite was carried out for 2 min, followed by washing with sterile distilled water according to [17].

REVISOR 2

Line 113:…. According to Lin et al. [17] (See entire manuscript for reference format)

121 changed

78 changed

Point  superíndice changed

155 changed

173

174

184 changed

221 changed

225 changed

249 changed

259 changed

318 super índice

438 eliminate “to”

443 deleted parenthesis

REVISOR 2

Check all references; some references are given incorrectly. For example: [17] should be like:

Lin TF, Huang HI, Shen FT, Young CC. Los protones del ácido glucónico son el principal factor responsable de la disolución del fosfato tricálcico por Burkholderia cepacia CC-Al74. Bioresour Technol. Mayo de 2006; 97 (7): 957-60. doi: 10.1016 / j.biortech.2005.02.017. Publicación electrónica del 13 de diciembre de 2005 PMID: 16356711.

no Ta-Fa et al.

Correct thanks!!

REVISOR 2

Line 114: According to [18], add the name

Made online 123 !!!

REVISOR 2

Line 115: Why only Clavibacter michiganensis ?, 2% sodium hypochlorite used for all types of microbes?

Clavibacter is a seed pathogen that any producer seeks to eliminate… .. extremely aggressive !!! …… it is possible that with this treatment some endophytic bacteria are also eliminated but it has not been reported !!

REVISOR 2

Lines 120-125, sentence too long, check it. Also describe the procedure for bacterial growth in the medium.

Línea 130-135 was changed

Then, seeds were placed in growth chambers containing fine sand and seed covered with a fine cap of peat-moss (Sunshine, Sun I Cry Horticulture Canada, Ltd.) at 25 ± 3 °C in dark conditions of 24 h at H.r. of 45. Once seeds emergence were located under greenhouse conditions (36° C, H.r. of 40%, and 3000 luxe) during 45 days. Before transplanting, seedlings were inoculated at 14 and 26 days after sowed (das), immersing the root system in a bacterial base solution of Azospirillum brasilense with 1 X 10⁸ CFU^.mL during 10 s.

Line 126: what does "for 10 s" mean

Seconds

Changed by 10”

REVISOR 2

The growing condition of the field side plants should be described in detail, temperature, lights, sowing, duration of growth, climatic irrigation, etc.

Lline 169 and table 2 was considered

The conditions (T° C, H.r., photsynthetically active radiation (Rfa: me^. M^-2 s^-1) and annual mean insolation (Ima^.h), on the shadow house conditions are indicate in Table 2.

REVISOR 2

Line 145: "according to [20], and fungi, according to [21]". Please see the full MS to see the reference format (INGREDIENTS)

Ready!!! Thanks

REVISOR 2

Line 148: How have you identified pathogenic fungi, molecular biology tools? Describe in detail. Why were other biological properties of the soil not measured? Microbial diversity, soil enzymes, etc.

In this study we only considered that in the transplant soil there were no plant pathogens ……… the tests were biochemical and taxonomic keys.

REVISOR 2

Line 169: 2.8. Treatments and experimental design ”This part should move to the top where the treatments were described.

To be more explicit, the same section was left

REVISOR 2

Line 181: "was calculated [22]:" okay? Check the citation format.

Your previous observation was considered

REVISOR 2

Line 184: “o the arc-sin” is not clear.

216 line

The sentence was restructured

REVISOR 2

Line 230: what does quantification of bacteria mean? The microbial diversity of the rhizosphere? Or the survival of inoculated bacteria. Clarify and review this part. Why were the samples not taken during the growing season, but only at harvest? All these measures should also be described in the study objective.

It refers to the fact that at the end of the experiment they wanted to corroborate the presence of Azospirillum

No other samplings were made, since inoculations were made in seed, in seedling and 60 and 90 days after germination

Line 130-169

REVISOR 2

Lines 312-316: This part is not clear. How have you distinguished inoculated bacteria from indigenous soil bacteria? Or was the total microbial count described?

It refers to the fact that at the end of the experiment WE wanted to corroborate the presence of Azospirillum

REVISOR 2

Lines 418-419: the results part should not be repeated. Describe in the methods section how the bacteria inoculated after the plant growth stage were determined.

Eliminated the two lines

Line 454

REVISOR 3

line 17 and 18. I don't see the need for this sentence in the abstract as it is not directly related to the topic. Also, this scientific approach could be used outside of Mexico or the region in Mexico where the research was conducted.

The sentence was restructured but without eliminating the sentence, to enhance and sequence the importance of the tomato from Mexico worldwide

have probiotic properties and based on this, since this spring-2020 summer season, Mexico

REVISOR 3

Line 101. The nutritional content of the fertilizer could be expressed in terms of percentage N, P, and K.

it is not common to express it in%.

The NPK (or N-P-K) index is the acronym for the relationship between the chemical elements Nitrogen (N), Phosphorus (P) and Potassium (K) that are commonly used in fertilizers.

REVISOR 3

paragraph 108. There needs to be an introduction to the use of A. brasilense for crops. How long has it been used? For what crops? What is the mode of action? How has it been productive? In other words, there needs to be a comprehensive paragraph describing the use of A. brasilense to justify this study.

58-60 was considered

REVISOR 3

Line 173. How many tomato plants per replicate? Or 1 plant constituted 1 of the 14 replicates? Please explain.

Line 200-2003 paragraph reestrcutured

An experimental design of random blocks with fourteen repetitions for each variable were considered under a factorial design (2 X 6), where factor A corresponds to the inoculum with Ab, while factor B corresponds to solarized manure; 504 experimental units evaluated were all the blocks.

REVISOR 3

Paragraph 246. Please explain the importance of seedling growth rate with first few days of emergence. How is this relevant

Is the same (in parentehsis was wrote emergence rate)

Line 284

Results according to the proposed methodology show that in the emergency variable (emergence rate), seed inoculated with Azospirillum brasilense (Ab)……..

REVISOR 3

Line 467. I think reference to SARs is not relevant to this study and should be removed.

We request that it be added to agree with the introduction of the importance of consuming probiotic horticultural products such as tomato, properties that strengthen the body and more in this international biosecurity contingency

lo cual es deseable en estos días en que la población mundial demanda ser alimentada con productos vegetales ricos en biomoléculas que mitiguen la agresividad de un agente causal como el SARS-Covid-19; importantes propiedades que, por sus efectos sobre la salud, sugieren que previenen los riesgos de adquirir enfermedades crónicas como el cáncer y las enfermedades cardiovasculares [69].

REVIWER

LINE

ANSWER

REVISOR 1

Over 50 % of used references were older than 10 years. Part "Matherials and methods" should be inproved, for exaple it is not clear how determination of lycpene was carried out?!

The oldest references were changed, it can be seen in the document in references

REVISOR 1

Only 1/3 references used in Discussion are issued in the past 5 years.

The changes in the older references were considered, it can be seen in the document in references

Line 91

Deleted parenthesis

Línea 96

Plastic mark included

REVISOR 2

Line 85: How does solarization affect microbes, p. Eg possible human pathogenic bacteria in manure? Many reports indicate the possible transfer of pathogens from manure in modified soil to vegetables.

In line 99 a sentence is attached enriching the effect of solarization on microorganisms

It is worth mentioning that solarized manure has the capacity that, when covered without  albedo plastics, the energy radiated by the sun generates temperatures between 64 and 74 ° C, causing pathogenic organisms such as fungi, bacteria, nematodes, weeds and insects to be killed and eliminated [17].

We can also justify that,

In aspects of food safety, and in organic crops, the manure that will be used goes through a sanitary certification where it shows the absence of food safety pathogens such as Klebsiella pnseuomiae, Salmonella spp, Campylobacter pp and Escherichia coli, Clostridium perfringens

A reference was added and consequently there was a numbering traversal

Salazar-Sosa E.; Fortis-Hernández, M.; López-Martínez, J.D.; Muños-Alvarado, C.L.; Lara-Macías, M.E.; Amado-Álvarez, J.P.; Vázquez-Vázquez,  C.; Trejo- Escareño, H.I.; Chavarría-Galicia, J.A. Comparison among solarization kind of pailes and their impact in the control of Cryptosporidium parvum and Giardia lamblia. International Research Journal of Agricultural Science and Soil Science. 2011. 1(9) 355-364.

REVISOR 2

Line 100: Add a source of chemical fertilizers

In line 109, a (source = reference) was added based on fertilization terms for this study

REVISOR 2

Line 107: Inoculation of tomato seeds with Azospirillum brasilense (Ab)

The title was changed

REVISOR 2

The variety could be described in the text.

On line 114 the following text was added:

In the present study, the variety TOP 2299 (Ahern Seeds®), type saladess, was evaluated; It is characterized by being a vigorous plant, strong bearing and with thick stems, medium internodes, with intermediate-late production cycles. Good development at high temperatures in spring-summer cycles. Fruits of large sizes, average 160 to 200 grams, maintain their size throughout the production cycle. In this phase, seeds were treated by means

REVISOR 2

Line 108: Tomato (Solanum lycopersicum, var aladette TOP 2299), check if correct, add botanical name and variety name.

Yes. It is correct

REVISOR 2

Line 108-112: Sentence too long, check it.

Paragraph was changed by:

In this phase, seeds were treated by means of a sterile distilled water wash to eliminate excess dyes and chemical products. Seeds were immersed in 70% alcohol for 10 sec and then carrying out three washes with sterile distilled water. Next, another disinfection with 2% sodium hypochlorite was carried out for 2 min, followed by washing with sterile distilled water according to [17].

REVISOR 2

Line 113:…. According to Lin et al. [17] (See entire manuscript for reference format)

121 changed

78 changed

Point  superíndice changed

155 changed

173

174

184 changed

221 changed

225 changed

249 changed

259 changed

318 super índice

438 eliminate “to”

443 deleted parenthesis

REVISOR 2

Check all references; some references are given incorrectly. For example: [17] should be like:

Lin TF, Huang HI, Shen FT, Young CC. Los protones del ácido glucónico son el principal factor responsable de la disolución del fosfato tricálcico por Burkholderia cepacia CC-Al74. Bioresour Technol. Mayo de 2006; 97 (7): 957-60. doi: 10.1016 / j.biortech.2005.02.017. Publicación electrónica del 13 de diciembre de 2005 PMID: 16356711.

no Ta-Fa et al.

Correct thanks!!

REVISOR 2

Line 114: According to [18], add the name

Made online 123 !!!

REVISOR 2

Line 115: Why only Clavibacter michiganensis ?, 2% sodium hypochlorite used for all types of microbes?

Clavibacter is a seed pathogen that any producer seeks to eliminate… .. extremely aggressive !!! …… it is possible that with this treatment some endophytic bacteria are also eliminated but it has not been reported !!

REVISOR 2

Lines 120-125, sentence too long, check it. Also describe the procedure for bacterial growth in the medium.

Línea 130-135 was changed

Then, seeds were placed in growth chambers containing fine sand and seed covered with a fine cap of peat-moss (Sunshine, Sun I Cry Horticulture Canada, Ltd.) at 25 ± 3 °C in dark conditions of 24 h at H.r. of 45. Once seeds emergence were located under greenhouse conditions (36° C, H.r. of 40%, and 3000 luxe) during 45 days. Before transplanting, seedlings were inoculated at 14 and 26 days after sowed (das), immersing the root system in a bacterial base solution of Azospirillum brasilense with 1 X 10⁸ CFU^.mL during 10 s.

Line 126: what does "for 10 s" mean

Seconds

Changed by 10”

REVISOR 2

The growing condition of the field side plants should be described in detail, temperature, lights, sowing, duration of growth, climatic irrigation, etc.

Lline 169 and table 2 was considered

The conditions (T° C, H.r., photsynthetically active radiation (Rfa: me^. M^-2 s^-1) and annual mean insolation (Ima^.h), on the shadow house conditions are indicate in Table 2.

REVISOR 2

Line 145: "according to [20], and fungi, according to [21]". Please see the full MS to see the reference format (INGREDIENTS)

Ready!!! Thanks

REVISOR 2

Line 148: How have you identified pathogenic fungi, molecular biology tools? Describe in detail. Why were other biological properties of the soil not measured? Microbial diversity, soil enzymes, etc.

In this study we only considered that in the transplant soil there were no plant pathogens ……… the tests were biochemical and taxonomic keys.

REVISOR 2

Line 169: 2.8. Treatments and experimental design ”This part should move to the top where the treatments were described.

To be more explicit, the same section was left

REVISOR 2

Line 181: "was calculated [22]:" okay? Check the citation format.

Your previous observation was considered

REVISOR 2

Line 184: “o the arc-sin” is not clear.

216 line

The sentence was restructured

REVISOR 2

Line 230: what does quantification of bacteria mean? The microbial diversity of the rhizosphere? Or the survival of inoculated bacteria. Clarify and review this part. Why were the samples not taken during the growing season, but only at harvest? All these measures should also be described in the study objective.

It refers to the fact that at the end of the experiment they wanted to corroborate the presence of Azospirillum

No other samplings were made, since inoculations were made in seed, in seedling and 60 and 90 days after germination

Line 130-169

REVISOR 2

Lines 312-316: This part is not clear. How have you distinguished inoculated bacteria from indigenous soil bacteria? Or was the total microbial count described?

It refers to the fact that at the end of the experiment WE wanted to corroborate the presence of Azospirillum

REVISOR 2

Lines 418-419: the results part should not be repeated. Describe in the methods section how the bacteria inoculated after the plant growth stage were determined.

Eliminated the two lines

Line 454

REVISOR 3

line 17 and 18. I don't see the need for this sentence in the abstract as it is not directly related to the topic. Also, this scientific approach could be used outside of Mexico or the region in Mexico where the research was conducted.

The sentence was restructured but without eliminating the sentence, to enhance and sequence the importance of the tomato from Mexico worldwide

have probiotic properties and based on this, since this spring-2020 summer season, Mexico

REVISOR 3

Line 101. The nutritional content of the fertilizer could be expressed in terms of percentage N, P, and K.

it is not common to express it in%.

The NPK (or N-P-K) index is the acronym for the relationship between the chemical elements Nitrogen (N), Phosphorus (P) and Potassium (K) that are commonly used in fertilizers.

REVISOR 3

paragraph 108. There needs to be an introduction to the use of A. brasilense for crops. How long has it been used? For what crops? What is the mode of action? How has it been productive? In other words, there needs to be a comprehensive paragraph describing the use of A. brasilense to justify this study.

58-60 was considered

REVISOR 3

Line 173. How many tomato plants per replicate? Or 1 plant constituted 1 of the 14 replicates? Please explain.

Line 200-2003 paragraph reestrcutured

An experimental design of random blocks with fourteen repetitions for each variable were considered under a factorial design (2 X 6), where factor A corresponds to the inoculum with Ab, while factor B corresponds to solarized manure; 504 experimental units evaluated were all the blocks.

REVISOR 3

Paragraph 246. Please explain the importance of seedling growth rate with first few days of emergence. How is this relevant

Is the same (in parentehsis was wrote emergence rate)

Line 284

Results according to the proposed methodology show that in the emergency variable (emergence rate), seed inoculated with Azospirillum brasilense (Ab)……..

REVISOR 3

Line 467. I think reference to SARs is not relevant to this study and should be removed.

We request that it be added to agree with the introduction of the importance of consuming probiotic horticultural products such as tomato, properties that strengthen the body and more in this international biosecurity contingency

which is desirable in these days when the world population demands to be fed with plant products rich in biomolecules that mitigate the aggressiveness of a causal agent such as SARS-Covid-19; important properties that, due to their health effects, suggest that they prevent the risks of acquiring chronic diseases such as cancer and cardiovascular diseases [69].

REVIWER

LINE

ANSWER

3

1.  The authors did not incorporate my reviewer concerns into their revision.  

They responded to the comments but did not make most corrections.  I strongly feel that these corrections need to be made.  

 2.   There was not a revision made to correct the numerous grammatical errors throughout the manuscript.  This needs to be done before I can accept the manuscript as complete and suitable.  

Dear Dr., Reviewer number 3, we apologize if we were not explicit at the time of making known how we made your observations.

WE REITERATE OUR APOLOGIES.

We want to clarify that on this second occasion, we developed your observations in another way ... in this new version and due to the changes that were made on the 1st occasion by all the reviewers, THE LINES THAT YOU INDICATE IN YOUR OBSERVATIONS ARE THEY WALKED ... IN EACH OBSERVATION THAT YOU MAKE US, IN THE THIRD COLUMN WE INDICATE YOU IN WHICH LINE THAT CHANGE WAS LEFT

3

Extensive editing of English language and style required

thank you for your fine observations to enrich the document.

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line 17 and 18. I don't see the need for this sentence in the abstract as it is not directly related to the topic. Also, this scientific approach could be used outside of Mexico or the region in Mexico where the research was conducted.

In new versión was

The sentence was restructured eliminating the requested sentence

…….. and based on this, since this spring-2020 summer season, Mexico has been monopolizing 24.1% of the world tomato trade

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Line 101. The nutritional content of the fertilizer could be expressed in terms of percentage N, P, and K.

In new versión was

In line 106-108, the fertilization formulas were restructured by adding the%

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paragraph 108. There needs to be an introduction to the use of A. brasilense for crops. How long has it been used? For what crops? What is the mode of action? How has it been productive? In other words, there needs to be a comprehensive paragraph describing the use of A. brasilense to justify this study.

In new versión was considered in line 125-138

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Line 173. How many tomato plants per replicate? Or 1 plant constituted 1 of the 14 replicates? Please explain.

Line 193-198 paragraph reestrcutured considering the observations

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Paragraph 246. Please explain the importance of seedling growth rate with first few days of emergence. How is this relevant

In new versión Line 267-271

Observation considering the next paragrapgh:

Results according to the proposed methodology show that in the emergency variable (emergence rate, which it is the germination energy of different seed treatments benefited in this case by Ab) and which consists of recording the "germination rate", that is, the number of days needed to achieve a high percentage of the germination capacity; the shorter this period, the greater the germination energy of the seed benefited by Ab), seed inoculated with Azospirillum brasilense (Ab) compared with treatment non inoculated, was more effective (p≤ 0.05) to response in emergency of seedling (Table 3); while in 2nd day treatment with Ab reached 100% in emergency of seedling, treatment non inoculated was obtained until four day before sowed.

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Line 467. I think reference to SARs is not relevant to this study and should be removed.

Observation considered

New versión

Line 490-494

Thanks