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Article

Effect of Greenhouse CO2 Supplementation on Yield and Mineral Element Concentrations of Leafy Greens Grown Using Nutrient Film Technique

by
Hardeep Singh
1,
Megha R. Poudel
1,*,
Bruce L. Dunn
1,
Charles Fontanier
1 and
Gopal Kakani
2
1
Department of Horticulture and Landscape Architecture, Oklahoma State University, Stillwater, OK 74078-6027, USA
2
Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK 74078, USA
*
Author to whom correspondence should be addressed.
Agronomy 2020, 10(3), 323; https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy10030323
Submission received: 27 January 2020 / Revised: 19 February 2020 / Accepted: 24 February 2020 / Published: 27 February 2020
(This article belongs to the Special Issue Practice of Hydroponics in Vegetable Production)
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Abstract

:
Carbon dioxide (CO2) concentration is reported to be the most important climate variable in greenhouse production with its effect on plant photosynthetic assimilation. A greenhouse study was conducted using a nutrient film technique (NFT) system to quantify the effect of two different levels of CO2 (supplemented at an average of 800 ppm and ambient at ~410 ppm) on growth and nutritional quality of basil (Ocimum basilicum L.) ‘Cardinal’, lettuce (Lactuca sativa L.) ‘Auvona’, and Swiss chard (Beta vulgaris L.) ‘Magenta Sunset’ cultivars. Two identical greenhouses were used: one with CO2 supplementation and the other serving as the control with an ambient CO2 concentration. The results indicate that supplemented CO2 could significantly increase the height and width of hydroponically grown leafy greens. Supplemented CO2 increased the fresh weight of basil ‘Cardinal’, lettuce ‘Auvona’, and Swiss chard ‘Magenta Sunset’ by 29%, 24.7%, and 39.5%, respectively, and dry weight by 34.4%, 21.4%, and 40.1%, respectively. These results correspond to a significant reduction in Soil Plant Analysis Development (SPAD) and atLEAF values, which represent a decrease in leaf chlorophyll content under supplemented CO2 conditions. Chlorophyll, nitrogen (N), phosphorus (P), and magnesium (Mg) concentrations were generally lower in plants grown in supplemented CO2 conditions, but the results were not consistent for each species. Supplemented CO2 reduced tissue N concentration for basil ‘Cardinal’ and lettuce ‘Auvona’ but not Swiss chard, while Mg concentration was reduced in supplemented CO2 for Swiss chard ‘Magenta Sunset’ only. In contrast, Fe concentration was increased under supplemented CO2 for basil ‘Cardinal’ only. These findings suggest CO2 supplementation could increase yield of leafy greens grown with hydroponics and have varying impact on different mineral concentrations among species.

1. Introduction

With an increasing human population, the demand for food is also increasing, while the arable land per capita is being reduced throughout the world [1]. Protected soilless culture can be a good approach to help in sustainable production for feeding the increasing population. Soilless culture can be defined as cultivation of nonaquatic plants without use of mineral soil as a growth substrate, while all essential plant nutrients are provided through a nutrient solution [2]. In recent years, different systems of soilless culture (e.g., hydroponics, aeroponics, gravel culture, and rockwool culture) have been adopted worldwide for food production [3]. Cleaner and longer postharvest life of hydroponic produce could be one of the reasons for this increase [4,5]. Soilless culture has also been viewed favorably for its greater efficiency of water use, due to lack of loss from runoff, infiltration, evaporation from soil [6], and increased nutrient efficiency from recycling the nutrient solution [7]. The nutrient film technique (NFT) developed by Allen Cooper and his colleagues in 1960 is among the more popular hydroponic systems for cultivation of leafy greens [8].
A soilless culture production system can be profitable, particularly if the grower maintains year-round production [9]. However, year-round production of green leafy vegetables may require supplemental lighting in the winter and shade in the summer [4]. Carbon dioxide supplementation can be equally beneficial as light supplementation due to its effect on plant photosynthetic assimilation [10]. However, CO2 supplementation is most beneficial in autumn, spring, and winter when the ventilation system is closed [11]. Mortensen [10] reported that the advantages of CO2 enrichment in the greenhouse atmosphere were detected as early as the nineteenth century, but the technique was not used commercially until the 1960s when both cheap sources of high-purity CO2 and gas-tight greenhouse constructions became available [10,12].
A study showed that the CO2 concentration inside a sealed greenhouse can be as low as 150 ppm during the day, and CO2 supplementation was required in winter for optimum production of cucumber (Cucumis sativus L.) [13]. Similarly, a 30% increase in photosynthesis assimilation and growth of lettuce (Lactuca sativa L.) was reported as a result of CO2 supplementation in the greenhouse environment [9,10]. Furthermore, Both and colleagues [9] reported that a CO2 level of 400–600 µmol mol−1 is suitable for hydroponic culture of lettuce. In addition to the benefits of increased production, CO2 supplementation is reported to reduce transpiration in lettuce [4].
Greenness of leafy vegetables is an indicator of carotene content [14] as well as an influential trait affecting consumer preference and acceptability [15]. It was reported that CO2 supplementation can result in increased or decreased leaf chlorophyll content of leaves [16,17]. At the same time, meta-analysis conducted by Dong et al. [18] reported that CO2 supplementation had no effect on chlorophyll concentration. Similarly, when basil (Ocimum basilicum L.) was grown under 1500 ppm CO2, interveinal chlorosis was observed due to the accumulation of large grains of starch [19]. Chlorophyll meter readings using a Soil Plant Analysis Development (SPAD) meter can serve as an indicator of greenness for green leafy vegetables [20]. In addition, these chlorophyll meters can serve as good non-destructive indicators of nutrient content of leafy greens as significant correlations between chlorophyll meter readings and nutrients like nitrogen (N), potassium (K), and phosphorus (P) have been reported in the literature [21,22,23]. Another important characteristic of leafy green vegetables is their nutritional quality, which is also reported to be affected by supplementation of CO2 in the growth environment. It is reported that CO2 supplementation results in a decrease in nitrate concentrations of about 26% and 19% in fruit and leafy vegetables, respectively [16].
Humans have been consuming herbs for thousands of years. In the past as well as today, herbs are used for cosmetic, medicinal, and culinary purposes [24]. Tyson et al. [25] reported that herbs cover more than 18% of greenhouse acreage in the United States and were the third most grown crop in greenhouses. Similarly, year-round productions of leafy greens in greenhouses are gaining in popularity in the United States [26]. Although many studies have covered herbs and leafy greens in greenhouse production, very few have explored how greenhouse CO2 supplementation affects the growth and nutritional quality of these crops. Therefore, the objective of this study is to determine the effect of greenhouse CO2 supplementation on yield and nutritional quality of lettuce, basil, and Swiss chard (Beta vulgaris L.) grown hydroponically in an NFT system.

2. Materials and Methods

2.1. Plant Material and Growth Conditions

Seeds of ‘Auvona’ open-heart romaine lettuce, ‘Magenta Sunset’ Swiss chard, and ‘Cardinal’ basil were obtained from Johnny’s selected seeds (Winslow, ME, USA). Seeds were sown in rockwool starter cubes (1.5 cm3) with 98 cubes to a sheet (Grodan, Milton, Ontario, Canada) on 20 January 2016 and were transplanted into NFT tables (Hydrocycle 4-inch Pro NFT series system; Growers Supply, Dyersville, IA, USA) on 25 February 2016 (35 days after seeds were sown) at the Oklahoma State University (OSU) Department of Horticulture and Landscape Architecture Research Greenhouses (Stillwater, OK, USA). Each table had 10 channels measuring 10 cm wide, 5 cm deep, and 366 cm long. Channel lids had 18 predrilled circular holes 3.5 cm in size spaced 20.3 cm on center. One transplant was placed in each slot with 15 plants per species per table. The NFT channels had a slope of 2.8% between the irrigation and drainage end, and the water flowing along this slope was collected in a tank and recirculated by a pump to the irrigation pipe. The experiment was repeated with an additional planting of seedlings on 28 February 2017.
Hydroponic fertilizer 5N-4.8P-21.6K (Peter’s, J.R. Peters Inc., Allentown, PA, USA) and calcium nitrate (Haifa North America, Altamonte Spring, FL, USA) were used as fertilizer [24]. Tap water with an electrical conductivity (EC) of 0.5 dS m−1 and a pH of 7.8 was used to prepare the nutrient solution. In the tank, 147.41 g of 5N-4.8P-21.6K and 97.5 g of calcium nitrate were added to make 150 ppm of N. In 2-week intervals, the tanks were flushed and refilled to remove the excess nutrient buildup. The EC of all the nutrient solutions was maintained at 1.5–2.5 dS m−1, and the pH was maintained at 5.5 to 6.5 as recommended by Singh and Dunn [27]. The pH and EC of each solution was checked and maintained every third day. The nutrient solution pH was maintained using pH up and pH down solutions (General Hydroponics, Santa Rosa, CA, USA), whereas EC was maintained by adding water if the EC was high and adding nutrient solution in the same proportion of both bags if the EC was less than the recommended limit.

2.2. Experimental Setup

The study was conducted in a split-plot design. Two identical greenhouses were used and one of the greenhouses was fitted with a natural gas-burning CO2 generator (Johnson Gas Appliances, Cedar Rapids, IA, USA) in the middle of the greenhouse. The CO2 generator was set to produce a daily average of 800 ppm of CO2 by burning natural gas (Figure 1) during supplementation period. The generator was automatic and turned on from 6:00 a.m. to 14:00 p.m. A CO2 monitor (FLIR Commercial System Inc., Nashua, NH, USA) monitored the CO2 concentration in both greenhouses. Both greenhouses were set at 21/18 °C day/night temperature and exposed to natural photoperiod resulting in a daily light integral of 12 to 14 mol m−2 d −1 as measured using a data logger (T & D Corporation, Nagano, Japan). The average relative humidity for the greenhouse was 28%. Each species with CO2 treatment had 15 replicate plants. Similar methods described above were followed for the second study.

2.3. Data Collection

Data were collected 46 days after transplanting of plugs in the NFT system. Each plant was scanned using two different chlorophyll meters (SPAD-502, Spectrum Technologies, Aurora, IL, USA; and atLEAF, FT Green, Wilmington, DE, USA) at the time of harvest. For each plant, SPAD and atLEAF readings were taken from three mature leaves representing the base, middle, and top of the plant. For each plant, the SPAD and atLEAF readings were recorded as the average of single readings at the tip, base, and blade leaves of a plant. Plant height (from top of the table to plant tip), diameter (average of diagonal width), specific leaf area (SLA), total leaf area, fresh weight, dry weight, and plant mineral element concentrations were measured. Total leaf area was measured using a LI-3000C area meter (LI-COR, Inc., Lincoln, NE, USA). Specific leaf area was calculated as the ratio of one-sided total leaf area to the total dry weight of a plant. For each species in an experimental unit, three samples were taken for leaf area measurement and the same samples were used for mineral element concentrations analysis. After area measurements, leaves were dried in an oven at 57 °C for 72 h to measure dry weight. The samples were then sent to the Soil, Water and Forage Analytical Laboratory at Oklahoma State University for analysis of leaf mineral element concentrations using a nutrient analyzer (TruSpec Carbon and Nitrogen Analyzer; LECO Corp., St. Joseph, MI, USA). For mineral element concentration, the plant material was digested on a digestion block at 115 °C with concentrated nitric acid. The resulting solution was analyzed for mineral element concentrations on an inductively coupled plasma spectrometer.

2.4. Statistical Analysis

The experiment was analyzed as a split-plot design repeated in time. The whole main plots were two CO2 concentrations (~400 and an average of 800 ppm) and subplots were assigned from three species (lettuce, basil, and Swiss chard). Statistical analysis was performed at p > 0.5 using SAS/STAT software (version 9.4; SAS Institute, Cary, NC, USA). Data were subjected to PROC MIXED and pdmix800, a macro program used to compute means. To compare differences between treatment means the Tukey–Kramer test was used.

3. Results

3.1. Basil

Under supplemented CO2 conditions, both the height and width of ‘Cardinal’ were greater as compared to ambient CO2 conditions (Table 1). Similarly, fresh weight of ‘Cardinal’ was also greater in supplemented CO2 conditions by 24.7%, over ambient CO2 conditions (Table 1). As a result, the dry weight of ‘Cardinal’ was also greater under supplemented CO2 conditions. ‘Cardinal’, grown under supplemented CO2 conditions, was greater in size based on leaf number (data not shown). Carbon dioxide supplementation resulted in a significant increase of total leaf area of ‘Cardinal’ by 41.9% (Table 1). The SLA for ‘Cardinal’ was greater in ambient CO2 conditions (260.7 cm2 g−1) as compared to supplemented CO2 conditions (160 cm2 g−1) (Table 1). Similarly, SPAD and atLEAF values in ambient CO2 conditions were greater by 5.1% and 6.2% over supplemented CO2 conditions, respectively (Table 1). The N concentration was lower in ‘Cardinal’ leaves produced under supplemented CO2 conditions, while the Fe concentration was greater (Table 2).

3.2. Lettuce

There was no significant difference in height and diameter of ‘Auvona’ between different CO2 treatments (Table 1). Fresh weight of ‘Auvona’ was greater (24.7%) in supplemented CO2 conditions as compared to ambient CO2 conditions (Table 1). ‘Auvona’ plants were compact and weighed more but were of equal size in visual appearance (Figure 2). However, a physiological disorder of tipburn on inner leaves at a later growth stage was observed under supplemented CO2 conditions, while the plants under ambient conditions were healthy. Total leaf area for ‘Auvona’ was also greater (22.6%) under supplemented CO2 conditions as compared to ambient CO2 conditions (Table 1). Therefore, the SLA of ‘Auvona’ was greater in supplemented CO2 and was 271.1 and 321.6 cm2 g−1 in ambient and supplemented CO2, respectively (Table 1). The SPAD values for ‘Auvona’ were greater in ambient CO2 conditions as compared to supplemented CO2 conditions (Table 1). However, there was no significant difference in atLEAF values between different CO2 treatments. For foliar mineral element concentrations, N and P concentrations were greater in ambient CO2 conditions, while there was no significant difference among the two CO2 treatments for concentrations of other mineral elements (Table 2).

3.3. Swiss Chard

For ‘Magenta Sunset’, CO2 supplementation also resulted in increased height and plant width (Table 1). A greater (39.5%) fresh weight under supplemented CO2 conditions was observed between ambient (296.8 g) and supplemented CO2 (414.1 g) conditions (Table 1). Due to the greater number of leaves and greater plant size, the total leaf area of ‘Magenta Sunset’ also increased by 34% under supplemented CO2 conditions (Table 1). In contrast to lettuce ‘Auvona’ and basil ‘Cardinal’, there was no significant difference in the SLA in ‘Magenta Sunset’. The SPAD and atLEAF values for ‘Magenta Sunset’ were greater under ambient CO2 conditions in comparison to supplemented CO2 conditions (Table 1). Among the different foliar mineral element concentrations, P and Mg concentrations were greater under ambient CO2 conditions as compared to supplemented CO2 conditions (Table 2).

4. Discussion

During winters, greenhouses are not ventilated in order to keep them warmer; may result in depletion of greenhouse CO2 concentrations below ambient CO2 concentrations and suppression of photosynthesis and growth of vegetables [28,29]. Therefore, if greenhouses are supplemented with CO2 during this period it can result in increased growth rate due to increased photosynthesis [30]. Similar to the present study, the growth rate of lettuce was reported to increase by 30% under supplemented CO2 conditions, presumably due to an increase in the rate of photosynthetic assimilation [31]. However, the response of C3 plants in terms of photosynthetic acclimation is specific [32] and shows a positive response up to a certain concentration of CO2 only. Above 800–1000 ppm, some species may reach a saturation point and net photosynthesis does not increase with increasing CO2 [33].
Supplemented CO2 increases carbohydrate sink size, which results in increased photosynthetic accumulation and vegetative growth of different crops. The biomass and dry matter production was also expected to increase due to increased photosynthetic assimilation and growth rate in all three species. As expected, all three species under supplemented CO2 showed a significant increase in fresh and dry matter production. Similarly, previous studies also reported an increase in dry weight production under supplemented CO2 conditions in hydroponically grown lettuce [8,34], basil [32], and greenhouse grown Swiss chard ‘Fordhook Giant’ [35] with CO2 concentrations of 1300 ppm, 1500 ppm, and 72.5 ± 2.2 Pascal, respectively.
Most prior CO2 related studies reported a decrease in the SLA of a plant. Due to the storage of starch in leaves, the leaves of peanuts (Arachis hypogaea L.) had greater dry weight at 800 and 1200 ppm as compared to ambient (400 ppm) and resulted in a higher SLA [33]. However, Harmens et al. [36] explained that a decrease in SLA simply cannot be explained through increased photosynthesis and accelerated growth of plants under supplemented CO2. Rather, SLA depends on how assimilates are distributed in shoots and roots during various growth stages. Thus, considering both root and shoot parameters in future studies will help in understanding species specific nature of partitioning of assimilates in the roots and shoots.
Similar to the present study, Gillig et al. [32] also reported a significant decrease in chlorophyll level in hydroponically grown basil when grown at 400 and 1500 ppm CO2. Similarly, development of interveinal chlorosis was observed due to the accumulation of large grains of starch in basil grown under 1500 ppm CO2 concentration [17]. The chlorophyll level under supplemented CO2 can be explained by movement of N to other sinks or it may be due to degradation of the chlorophyll [37]. Another possible reason explaining a decrease in chlorophyll content is accumulation of non-structural carbohydrates under supplemented CO2 [38]. These non-structural carbohydrate accumulations are generally thought to physically distort the chloroplast [39].
Tissue N concentration of above-ground tissue is reported to be lowered by 10–15% as a result of CO2 supplementation in many species [40,41]. Studies related to leaf nutrient content in cotton (Gossypium hirsutum L.) [42], chrysanthemum (Chrysanthemum × morifolium Ramat.‘Fiesta’) [43], and hydroponically grown lettuce (‘Mantilla’) [44] reported a decrease in leaf N and P concentrations, which corresponds to results in our study, but the response was inconsistent among species. A robust single mechanism for lower nutrient content under supplemented CO2 has not yet been developed. The hypothesis has been described in previous studies which include dilution of N due to increased carbohydrates, decreased N uptake, decreased N demand, and reduced transpiration [45] of crops [41,46]. It was reported that CO2 supplementation limits the uptake of N and the synthesis of nitrogenous compounds of vegetables by 9.5% [47]. In the literature, it was reported among different mineral elements that Fe concentration experienced the greatest decrease (31%) in leafy greens grown under supplemented CO2 conditions [17]. In the current study, contrasting results were seen for basil ‘Cardinal’ where Fe concentration increased significantly in CO2 supplemented conditions. Similarly, an increase in leaf Fe concentration in hydroponically grown lettuce (‘Mantilla’) was reported under supplemented CO2 conditions [44]. The possible explanation for this increase in Fe concentration in some cases could be an increase in root nitric oxide levels under Fe-limited and elevated CO2 conditions [48]. Nitric oxide was indicated as a signal molecule involved in playing a role in regulating gene expression during Fe deficiency [49]. It is possible that the Fe concentration in the nutrient solution may have gone below the basil requirement and increased nitric oxide in roots may have upregulated Fe acquisition response under supplemented CO2 conditions. However, an increase in Fe concentration of leafy greens when grown in supplemented CO2 has beneficial effects for human nutrition [50]. Duval et al. [46] reported that the effect of supplemented CO2 on plant nutrient content depends on available N, tissue type, species, and nutrient ions. Some nutrients respond well, and some are not affected by supplemented CO2.
Tipburn is a physiological disorder in lettuce which is generally associated with distribution of calcium (Ca) ions within plant leaves [51]. A lower rate of transpiration and high humidity are environmental factors affecting Ca uptake and cause localized tipburn. The use of horizontal air flow (HAF) fans in the greenhouse can be a potential solution to decrease tip burn losses by increasing transpiration and lowering relative humidity. Another reason for the development of necrotic brown spots in the margin of developing leaves is an inability to meet Ca demand of a quickly growing plant (due to supplemented CO2, high light intensity, and greater fertilizer rate) causing lower distribution of Ca to inner leaves [52]. Additionally, Gilliham et al. [53] reported that translocation of Ca in plants is predominated by an apoplastic pathway and rate of transpiration determines the Ca concentration in plant tissue. The distribution of Ca in plant tissue is heterogeneous and the concentration of Ca might differ between inner and outer leaves depending upon the growing environment [54]. Plants grown with supplemental CO2 show lower rates of transpiration due to reduced stomatal conductance. A lower rate of transpiration might have resulted in a lower Ca concentration in inner leaves resulting in tipburn [8]. Although there was no significant difference in Ca concentration of plants grown in ambient and supplemented CO2 during whole plant mineral elements analysis, there might be a difference in inner and outer leaf Ca concentrations, which was not considered during the study. Since environmental factors (lower transpiration and higher humidity) could be the cause of tipburn under supplemented CO2; vertical air flow within greenhouses could be a feasible solution for the tipburn problem [52].

5. Conclusions

Results suggest that supplemented CO2 has significant potential to increase growth and development of leafy greens grown in NFT systems. Increased growth rate could result in early harvest and more crop cycles each year and thereby help in feeding the increasing world population. The growth response of different species varied, but this study showed increased growth of all three species. Supplementing CO2 in greenhouse environments during growth of hydroponically grown leafy greens may also result in lighter green (due to low chlorophyll content) produce which may impact the marketability of the produce. Physiological disorders such as tipburn in ‘Auvona’ may also reduce produce quality when grown under supplemented CO2 conditions. For mineral concentrations, the study suggests that CO2 supplementation may have both a positive and negative effect as lower leaf N concentration might affect available protein, while greater Fe concentration in our food when grown with a nutrient solution containing 2.30 ppm of Fe is a desired quality. Thus, future studies should examine the nutritional aspect and physiological changes in nutrient and water uptake of crops grown in supplemented CO2 conditions and what role HAF fans and air movement have on overall plant quality.

Author Contributions

Conceptualization, B.L.D. and M.R.P.; methodology, M.R.P.; software, M.R.P.; validation, H.S.; formal analysis, M.R.P.; investigation, B.L.D. and H.S.; resources, B.L.D.; writing—original draft preparation, M.R.P.; writing—review and editing, B.L.D.; H.S.; C.F.; G.K.; supervision, B.L.D.; project administration, B.L.D.; funding acquisition, B.L.D. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the USDA National Institute of Food and Agriculture, Hatch project, and the Division of Agricultural Sciences and Natural Resources at Oklahoma State University.

Acknowledgments

The authors are thankful to Stephen Stanphill for his support in conducting experiments at greenhouse.

Conflicts of Interest

The authors declare no conflicts of interest.

References

  1. Gruda, N.S. Increasing sustainability of growing media constituents and stand-alone substrates in soilless culture systems. Agronomy 2019, 9, 298. [Google Scholar] [CrossRef] [Green Version]
  2. Maxwell, K. Soilless (hydroponic) culture the past, present and future. An Australian viewpoint. In Soilless Culture; International Society for Soilless Culture: Wageningen, The Netherlands, 1986; pp. 27–34. [Google Scholar]
  3. Bradley, P.; Marulanda, C. Simplified hydroponics to reduce global hunger. Acta Hort. 2000, 554, 289–295. [Google Scholar] [CrossRef]
  4. Both, A. Ten Years of Hydroponic Lettuce Research; Department of Plant Biology and Pathology; The State University of New Jersey: New Brunswick, NJ, USA, 1998; pp. 7–14. Available online: http://www.researchgate.net/publication/266453402_TEN_YEARS_OF_HYDROPONIC_LETTUCE_RESEARCH (accessed on 2 January 2020).
  5. Succop, C.E.; Newman, S.E. Organic fertilization of fresh market sweet basil in a greenhouse. HortTechnology 2004, 14, 235–239. [Google Scholar] [CrossRef] [Green Version]
  6. Putra, P.A.; Yuliando, H. Soilless culture system to support water use efficiency and product quality: A review. Agric. Agric. Sci. Procedia 2015, 3, 283–288. [Google Scholar] [CrossRef] [Green Version]
  7. Christie, E. Water and Nutrient Reuse within Closed Hydroponic Systems.Graduate Thesis, Georgia Southern University, United States. Electron. Thesis Diss. 2014. Available online: https://digitalcommons.georgiasouthern.edu/cgi/viewcontent.cgi?article=2154&context=etd (accessed on 2 January 2020).
  8. Resh, H.M. Hydroponic Food Production: A Definitive Guidebook for the Advanced Home Gardener and the Commercial Hydroponic Grower; CRC Press: Boca Raton, FL, USA, 2012. [Google Scholar]
  9. Both, A.J.; Albright, L.D.; Langhans, R.W. Coordinated management of daily PAR integral and carbon dioxide for hydroponic lettuce productions. Acta Hort. 1998, 456, 45–51. [Google Scholar] [CrossRef]
  10. Mortensen, L.M. CO2 enrichment in greenhouses. Crop responses. Sci. Hort. 1987, 33, 1–25. [Google Scholar] [CrossRef]
  11. Willits, D.; Peet, M. Predicting yield responses to different greenhouse CO2 enrichment schemes: Cucumbers and tomatoes. Agric. For. Meteorol. 1989, 44, 275–293. [Google Scholar] [CrossRef]
  12. Hand, D.W. Crop responses to winter and summer CO2 enrichment. Acta Horticulturae 1984, 162, 45–64. [Google Scholar] [CrossRef]
  13. Klaring, H.P.; Hauschild, C.; Heibner, A.; Bar-Yosef, B. Model-based control of CO2 concentration in greenhouses at ambient levels increases cucumber yield. Agric. For. Meteorol. 2007, 143, 208–216. [Google Scholar] [CrossRef]
  14. Vittum, M.T. Effect of fertilizers on the quality of vegetables. Agron. J. 1963, 55, 425–429. [Google Scholar] [CrossRef]
  15. Ali, M.B.; Khandaker, L.; Oba, S. Comparative study on functional components, antioxidant activity and color parameters of selected colored leafy vegetables as affected by photoperiods. J. Food Agric. Environ. 2009, 7, 392–398. [Google Scholar]
  16. Li, Y.; Gupta, G. Photosynthetic changes in soybean with and without nitrogen and increased carbon dioxide. Plant Sci. 1993, 89, 1–4. [Google Scholar] [CrossRef]
  17. Zhao, X.; Mao, Z.; Xu, J. Gas exchange, chlorophyll and growth responses of Betula Platyphylla seedlings to elevated CO2 and nitrogen. Int. J. Biol. 2010, 2, 143–149. [Google Scholar] [CrossRef]
  18. Dong, J.; Gruda, N.; Lam, S.K.; Li, X.; Duan, Z. Effects of elevated CO2 on nutritional quality of vegetables–a review. Front. Plant Sci. 2018, 9, 924. [Google Scholar] [CrossRef]
  19. Holbrook, G.P.; Hansen, J.; Wallick, K.; Zinnen, T.M. Starch accumulation during hydroponic growth of spinach and basil plants under carbon dioxide enrichment. Environ. Exp. Bot. 1993, 33, 313–321. [Google Scholar] [CrossRef]
  20. Colonna, E.; Rouphael, Y.; Barbieri, G.; De Pascale, S. Nutritional quality of ten leafy vegetables harvested at two light intensities. Food Chem. 2016, 199, 702–710. [Google Scholar] [CrossRef]
  21. Dunn, B.L.; Singh, H.; Goad, C. Relationship between chlorophyll meter readings and nitrogen in poinsettia leaves. J. Plant Nutr. 2018, 41, 1566–1575. [Google Scholar] [CrossRef]
  22. Singh, H.; Dunn, B.; Payton, M.; Brandenberger, L. Fertilizer and cultivar selection of lettuce, basil, and swiss chard for hydroponic production. HortTechnology 2019, 29, 50–56. [Google Scholar] [CrossRef] [Green Version]
  23. Dunn, B.L.; Singh, H.; Payton, M.; Kincheloe, S. Effects of nitrogen, phosphorus, and potassium on SPAD-502 and atLEAF sensor readings of Salvia. J. Plant Nutr. 2019, 41, 1674–1683. [Google Scholar] [CrossRef]
  24. Stapleton, S.C.; Hochmuth, R.C. Greenhouse production of several fresh-cut herbs in vertical hydroponic systems in north central Florida. Proc. Fla. State Hort. Soc. 2001, 114, 332–334. [Google Scholar]
  25. Tyson, R.V.; Hochmuth, R.C.; Lamb, E.M.; Hochmuth, G.J.; Sweat, M.S. A decade of change in Florida’s greenhouse vegetable industry: 1991–2001. Proc. Fla. State Hortic. Soc. 2001, 114, 280–283. [Google Scholar]
  26. Parkell, N.B.; Hochmuth, R.C.; Laughlin, W.L. Leafy Greens in Hydroponics and Protected Culture for Florida; University of Florida, IFAS Extension: Gainesville, FL, USA, 2016; pp. 1–7. [Google Scholar]
  27. Singh, H.; Dunn, B. Electrial Conductivity and pH Guide for Hydroponics; Oklahoma Coop. Exten. Fact Sheets, HLA-6722; Oklahoma State University, Division of Agricultural Sciences and Natural Resources: Stillwater, OK, USA, 2016. [Google Scholar]
  28. Li, X.; Dong, J.; Gruda, N.S.; Chu, W.; Duan, Z. Interactive effects of the CO2 enrichment and nitrogen supply on the biomass accumulation, gas exchange properties, and mineral elements concentrations in cucumber plants at different growth stages. Agronomy 2020, 10, 139. [Google Scholar] [CrossRef] [Green Version]
  29. Slack, G.; Hand, D.W. The effect of winter and summer CO2 enrichment on the growth and fruit yield of glasshouse cucumber. J. Hort. Sci. 1985, 60, 507–516. [Google Scholar] [CrossRef]
  30. Becker, C.; Kläring, H.P. CO2 enrichment can produce high red leaf lettuce yield while increasing most flavonoid glycoside and some caffeic acid derivative concentrations. Food Chem. 2016, 199, 736–745. [Google Scholar] [CrossRef] [Green Version]
  31. Hunt, R.; Wilson, J.W.; Hand, D.W.; Sweeney, D.G. Integrated analysis of growth and light interception in winter lettuce I. Analytical methods and environmental influences. Ann. Bot. 1984, 54, 743–757. [Google Scholar] [CrossRef]
  32. Gillig, S.; Heinemann, R.; Hurd, G.; Pittore, K.; Powell, D. Response of basil (Ocimum basilicum) to Increased CO2 Levels. Available online: http://jvarekamp.web.wesleyan.edu/CO2/CO2%20Basil%20Final%20Master.pdf (accessed on 2 January 2020).
  33. Stanciel, K.; Mortley, D.G.; Hileman, D.R.; Loretan, P.R.; Bonsi, C.K.; Hill, W.A. Growth, pod, and seed yield, and gas exchange of hydroponically grown peanut in response to CO2 enrichment. HortScience 2000, 35, 49–52. [Google Scholar] [CrossRef] [Green Version]
  34. He, J.; Austin, P.T.; Lee, S.K. Effects of elevated root zone CO2 and air temperature on photosynthetic gas exchange, nitrate uptake, and total reduced nitrogen content in aeroponically grown lettuce plants. J. Exp. Bot. 2010, 61, 3959–3969. [Google Scholar] [CrossRef] [Green Version]
  35. Ziska, L.H.; Sicher, R.C.; Kremer, D.F. Reversibility of photosynthetic acclimation of Swiss chard and sugar beet grown at elevated concentrations of CO2. Physiol. Plantarum 1995, 95, 355–364. [Google Scholar] [CrossRef]
  36. Harmens, H.; Stirling, C.M.; Marshall, C.; Farrar, J.F. Is partitioning of dry weight and leaf area within Dactylis glomerata affected by N and CO2 enrichment? Ann. Bot. 2000, 86, 833–839. [Google Scholar] [CrossRef] [Green Version]
  37. Kumari, S.; Agrawal, M.; Tiwari, S. Impact of elevated CO2 and elevated O3 on Beta vulgaris L.: Pigments, metabolites, antioxidants, growth and yield. Environ. Pollut. 2013, 174, 279–288. [Google Scholar] [CrossRef]
  38. Jeong, H.M.; Kim, H.R.; Hong, S.; You, Y.H. Effects of elevated CO2 concentration and increased temperature on leaf quality responses of rare and endangered plants. J. Ecol. Environ. 2018, 42, 1. [Google Scholar] [CrossRef] [Green Version]
  39. Delucia, E.H.; Sasek, T.W.; Strain, B.R. Photosynthetic inhibition after long-term exposure to elevated levels of atmospheric carbon dioxide. Photosynth. Res. 1985, 7, 175–184. [Google Scholar] [CrossRef]
  40. Cotrufo, M.F.; Ineson, P.; Scott, A. Elevated CO2 reduces the nitrogen concentration of plant tissues. Glob. Chang. Biol. 1998, 4, 43–54. [Google Scholar] [CrossRef]
  41. Taub, D.R.; Wang, X. Why are nitrogen concentrations in plant tissues lower under elevated CO2? A critical examination of the hypotheses. J. Integr. Plant Biol. 2008, 50, 1365–1374. [Google Scholar] [CrossRef]
  42. Huluka, G.; Hileman, D.R.; Biswas, P.K.; Lewin, K.F.; Nagy, J.; Hendrey, G.R. Effects of elevated CO2 and water stress on mineral concentration of cotton. Agric. For. Meteorol. 1994, 70, 141–152. [Google Scholar] [CrossRef]
  43. Kuenhy, J.S.; Peet, M.M.; Melson, P.V.; Willits, D. Nutrient dilution by starch in CO2 enriched Chrysanthemum. J. Exp. Bot. 1991, 42, 711–716. [Google Scholar]
  44. Chagvardieff, P.; D’Aletto, T.; Andre, M. Specific effects of irradiance and CO2 concentration doublings on productivity and mineral content in lettuce. Adv. Space Res. 1994, 14, 269–275. [Google Scholar] [CrossRef]
  45. Jin, C.W.; Du, S.T.; Chen, W.W.; Li, G.X.; Zhang, Y.S.; Zheng, S.J. Elevated carbon dioxide improves plant iron nutrition through enhancing the iron-deficiency-induced responses under iron-limited conditions in tomato. Plant Physiol. 2009, 150, 272–280. [Google Scholar] [CrossRef] [Green Version]
  46. Graziano, M.; Lamattina, L. Nitric oxide accumulation is required for molecular and physiological responses to iron deficiency in tomato roots. Plant J. 2007, 52, 949–960. [Google Scholar] [CrossRef]
  47. McDonald, E.P.; Erickson, J.E.; Kruger, E.L. Can decreased transpiration limit plant nitrogen acquisition in elevated CO2? Funct. Plant Biol. 2002, 29, 1115–1120. [Google Scholar] [CrossRef]
  48. Duval, B.D.; Blankinship, C.J.; Dijkstra, P.; Hungate, B.A. CO2 effects on plant nutrient concentration depend on plant functional group and available nitrogen: A meta-analysis. Plant Ecol. 2012, 213, 505–521. [Google Scholar] [CrossRef]
  49. Taub, D.R.; Miller, B.; Allen, H. Effects of elevated CO2 on the protein concentration of food crops: A meta-analysis. Glob. Chang. Biol. 2008, 14, 565–575. [Google Scholar] [CrossRef]
  50. Chiplonkar, S.A.; Tarwadi, K.V.; Kavedia, R.B.; Mengale, S.S.; Paknikar, K.M.; Agte, V.V. Fortification of vegetarian diets for increasing bioavailable iron density using green leafy vegetables. Food Res. Int. 1999, 32, 169–174. [Google Scholar] [CrossRef]
  51. Assimakopoulou, A.; Kotsiras, A.; Nifakos, K. Incidence of lettuce tipburn as related to hydroponic system and cultivar. J. Plant Nutr. 2013, 36, 1383–1400. [Google Scholar] [CrossRef]
  52. Mattson, N.S. Tipburn of Hydroponic Lettuce e-Gro Alert. Available online: https://www.e-gro.org/pdf/2015_431.pdf (accessed on 2 January 2020).
  53. Gilliham, M.; Dayod, M.; Hocking, B.J.; Xu, B.; Conn, S.J.; Kaiser, B.N.; Leigh, R.A.; Tyerman, S.D. Calcium delivery and storage in plant leaves: Exploring the link with water flow. J. Exp. Bot. 2011, 62, 2233–2250. [Google Scholar] [CrossRef]
  54. Lee, J.G.; Choi, C.S.; Jang, Y.A.; Jang, S.W.; Lee, S.G.; Um, Y.C. Effects of air temperature and air flow rate control on the tipburn occurrence of leaf lettuce in a closed-type plant factory system. Hort. Environ. Biotechnol. 2013, 54, 303–310. [Google Scholar] [CrossRef]
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Figure 1. Average daily value of ambient and supplemental CO2 concentration measured at Oklahoma State University (OSU) Department of Horticulture and Landscape Architecture Research Greenhouses (Stillwater, OK, USA) during the study period (average of 2016 and 2017).
Figure 1. Average daily value of ambient and supplemental CO2 concentration measured at Oklahoma State University (OSU) Department of Horticulture and Landscape Architecture Research Greenhouses (Stillwater, OK, USA) during the study period (average of 2016 and 2017).
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Figure 2. Basil ‘Cardinal’, lettuce ‘Auvona’, and Swiss chard ‘Magenta Sunset’ (left to right in both figures) grown in (a) ambient and (b) supplemented CO2 condition 46 days after transplanting.
Figure 2. Basil ‘Cardinal’, lettuce ‘Auvona’, and Swiss chard ‘Magenta Sunset’ (left to right in both figures) grown in (a) ambient and (b) supplemented CO2 condition 46 days after transplanting.
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Table
Table 1. Effect of CO2 treatments (ambient at 400 ppm and supplemented at an average of 800 ppm) on height, width, fresh weight, dry weight, Soil Plant Analysis Development (SPAD), atLEAF, total leaf area, and specific leaf area values for lettuce ‘Auvona’, Swiss chard ‘Magenta Sunset’, and basil ‘Cardinal’ grown under nutrient film technique (NFT) in Stillwater, OK, USA in 2016 and 2017.
Table 1. Effect of CO2 treatments (ambient at 400 ppm and supplemented at an average of 800 ppm) on height, width, fresh weight, dry weight, Soil Plant Analysis Development (SPAD), atLEAF, total leaf area, and specific leaf area values for lettuce ‘Auvona’, Swiss chard ‘Magenta Sunset’, and basil ‘Cardinal’ grown under nutrient film technique (NFT) in Stillwater, OK, USA in 2016 and 2017.
Carbon DioxideHeight (cm)Width (cm)Fresh Weight (g)Dry Weight (g)SPAD (unitless)atLEAF (Unitless)Total Leaf Area (cm2)Specific Leaf Area z (cm2 g−1)
Basil
Ambient34.0b y26.2b y123.1b y11.9b y44.4a y52.6a y1644.4b x260.7a x
Elevated36.9a29.8a158.9a15.0a42.2b49.6b2333.8a160.0b
Lettuce
Ambient26.6a25.2a203.8b19.1b48.5a45.8a4884.5b271.1b
Elevated26.5a25.7a254.2a23.8b45.1b48.6a5988.5a321.6a
Swiss chard
Ambient48.4b33.3b296.8b26.0b50.6a54.8a2836.4b105.9a
Elevated52.8a35.4a414.1a38.4a47.4b51.0b3801.1a105.8a
z Specific leaf area is the ratio of leaf area of one side of an individual leaf to the dry weight of the same leaf; y Means (n = 30) within a parameter of an individual species followed by the same letter are not significantly different at p ≤ 0.05; x Means (n = 10) within a parameter of an individual species followed by the same letter are not significantly different at p ≤ 0.05.
Table
Table 2. Effect of CO2 treatments (ambient at 400 ppm and supplemented at an average of 800 ppm) on mineral element concentrations of lettuce ‘Auvona’, Swiss chard ‘Magenta Sunset’, and basil ‘Cardinal’ grown under nutrient film technique (NFT) in Stillwater, OK, USA in 2016 and 2017.
Table 2. Effect of CO2 treatments (ambient at 400 ppm and supplemented at an average of 800 ppm) on mineral element concentrations of lettuce ‘Auvona’, Swiss chard ‘Magenta Sunset’, and basil ‘Cardinal’ grown under nutrient film technique (NFT) in Stillwater, OK, USA in 2016 and 2017.
Carbon dioxideNitrogen (%)Phosphorus (%)Calcium (%)Potassium (%)Magnesium (%)Sulphur (%)Boron (ppm)Manganese (ppm)Iron (ppm)Zinc (ppm)
Basil
Ambient5.1a z0.6a2.6a2.97a0.86a0.30a36.70a57.96a135.38b49.90a
Elevated4.6b0.5a2.5a3.21a0.84a0.29a40.46a55.93a239.30a48.75a
Lettuce
Ambient 4.3a0.6a1.8a5.68a0.63a0.28a49.93a99.90a154.40a38.90a
Elevated3.7b0.4b1.6a5.12a0.81a0.26a47.51a95.20a195.01a32.45a
Swiss chard
Ambient 4.6a0.3b1.7a4.38a1.14a0.37a61.93a108.03a97.01a35.75a
Elevated4.4a0.5a1.4a4.55a0.70b0.33a59.68a71.08a137.55a39.96a
z For each species, means (n = 6) within a column with the same letters are not significantly different at p ≤ 0.05.

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Singh, H.; Poudel, M.R.; Dunn, B.L.; Fontanier, C.; Kakani, G. Effect of Greenhouse CO2 Supplementation on Yield and Mineral Element Concentrations of Leafy Greens Grown Using Nutrient Film Technique. Agronomy 2020, 10, 323. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy10030323

AMA Style

Singh H, Poudel MR, Dunn BL, Fontanier C, Kakani G. Effect of Greenhouse CO2 Supplementation on Yield and Mineral Element Concentrations of Leafy Greens Grown Using Nutrient Film Technique. Agronomy. 2020; 10(3):323. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy10030323

Chicago/Turabian Style

Singh, Hardeep, Megha R. Poudel, Bruce L. Dunn, Charles Fontanier, and Gopal Kakani. 2020. "Effect of Greenhouse CO2 Supplementation on Yield and Mineral Element Concentrations of Leafy Greens Grown Using Nutrient Film Technique" Agronomy 10, no. 3: 323. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy10030323

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