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Article

Investigation of Sample Size Estimation for Measuring Quantitative Characteristics in DUS Testing of Shiitake Mushrooms

1
Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
2
Shanghai Sub-Center for New Plant Variety Tests, MARA, Shanghai 201415, China
3
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
4
Development Center of Science and Technology, MARA, Beijing 061110, China
*
Author to whom correspondence should be addressed.
These authors are contributed equally to this work.
Submission received: 24 April 2024 / Revised: 20 May 2024 / Accepted: 24 May 2024 / Published: 25 May 2024

Abstract

The sampling technique is commonly used in research investigations to more accurately estimate data with greater precision, at a lower cost and in less time. In plant DUS (distinctness, uniformity, and stability) testing, many quantitative characteristic data usually need to be obtained through individual measurements. However, there is currently no scientific method for determining the appropriate sampling size. The minimum number of testing samples for DUS testing was calculated based on the theory of sample size in descriptive studies and was validated through simple random sampling. The results show that the quantitative characteristics for the edible mushroom Shiitake (Lentinula edodes) in DUS testing were uniform. The calculated results show that 10 fruiting bodies for a single measurement were sufficient. Furthermore, the outcomes of the random sampling revealed that the mean of 10 samples did not significantly differ from the mean of all data. When the sample size exceeded 10, Cohen’s kappa statistic suggested that the conclusion of distinctness was very close to the near-perfect agreement. Reducing the number of samples did not affect the uniform assessment. This study suggests that the theory of sample size in descriptive studies could be applied to calculate the minimum sample size in DUS testing, and for Shiitake DUS testing, measuring 10 fruiting bodies was sufficient.
Keywords: sample size; quantitative characteristics; DUS sample size; quantitative characteristics; DUS

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MDPI and ACS Style

Deng, S.; Zhang, M.; Li, A.; Ren, L.; Zhang, Y.; Zhao, H.; Zhang, Y.; Song, C.; Han, R.; Tan, Q.; et al. Investigation of Sample Size Estimation for Measuring Quantitative Characteristics in DUS Testing of Shiitake Mushrooms. Agronomy 2024, 14, 1130. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy14061130

AMA Style

Deng S, Zhang M, Li A, Ren L, Zhang Y, Zhao H, Zhang Y, Song C, Han R, Tan Q, et al. Investigation of Sample Size Estimation for Measuring Quantitative Characteristics in DUS Testing of Shiitake Mushrooms. Agronomy. 2024; 14(6):1130. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy14061130

Chicago/Turabian Style

Deng, Shan, Meiyan Zhang, Aiai Li, Li Ren, Yiying Zhang, Hong Zhao, Yu Zhang, Chunyan Song, Ruixi Han, Qi Tan, and et al. 2024. "Investigation of Sample Size Estimation for Measuring Quantitative Characteristics in DUS Testing of Shiitake Mushrooms" Agronomy 14, no. 6: 1130. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy14061130

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