Highly Specific Memory B Cells Generation after the 2nd Dose of BNT162b2 Vaccine Compensate for the Decline of Serum Antibodies and Absence of Mucosal IgA
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethical Approval
2.2. Human Subjects
2.3. Cell Isolation and Cryopreservation
2.4. Polyclonal Memory B-Cell Stimulation
2.5. B Cell ELISpot
2.6. ELISA for Specific IgA and IgM Detection
2.7. Quantitative Determination of Anti-N, Anti-S, Trimeric Spike and RBD Antibodies
2.8. Detection of Antigen-Specific Memory B Cells
2.9. SARS-CoV-2 Variant Determination
2.10. Statistical Analysis
3. Results
3.1. Serum Antibodies
3.2. Memory B Cell Detected by ELISpot
3.3. Spike-Specific Memory B Cells Detected by Flow Cytometry
3.4. IgA Is not Produced at Mucosal Sites after Vaccination with BNT162b2
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data and Materials Availability
Acknowledgments
Conflicts of Interest
References
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Study Group | Memory B Cells by ELISpot | Memory B Cells by FACS | Surrogate Neutralization | |
---|---|---|---|---|
Number of HCW | 108 | 74 | 34 | 70 |
Age (mean ± SD) | 46.95 ± 11.35 | 47.04 ± 10.93 | 45.7 ± 11.96 | 48.11 ± 11.45 |
Sex (M/F) | 31/77 | 17/57 | 14/20 | 17/53 |
Number of patients | Assay | Total Patients Followed Over Time |
ELISpot | 74 | |
FACs | 34 | |
Surrogate Neutralization | 70 | |
ELISA | 108 |
Study Group | |
---|---|
Mothers’ age (mean ± SD) | 33.36 ± 4.17 |
Mothers’ breast milk | 7 not vaccinated 16 vaccinated |
HCWs Age (Mean ± SD) | 45.6 ± 6.5 |
Sex | 66.7% F; 33.3% M |
SARS-CoV-2 infection | 33.3% asymptomatic; 50% paucisymptomatic; 16.7% mild |
SARS-CoV-2 variant | 66.6% Gamma; 16.7% Alpha; 16.7% n.a. |
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Piano Mortari, E.; Russo, C.; Vinci, M.R.; Terreri, S.; Fernandez Salinas, A.; Piccioni, L.; Alteri, C.; Colagrossi, L.; Coltella, L.; Ranno, S.; et al. Highly Specific Memory B Cells Generation after the 2nd Dose of BNT162b2 Vaccine Compensate for the Decline of Serum Antibodies and Absence of Mucosal IgA. Cells 2021, 10, 2541. https://0-doi-org.brum.beds.ac.uk/10.3390/cells10102541
Piano Mortari E, Russo C, Vinci MR, Terreri S, Fernandez Salinas A, Piccioni L, Alteri C, Colagrossi L, Coltella L, Ranno S, et al. Highly Specific Memory B Cells Generation after the 2nd Dose of BNT162b2 Vaccine Compensate for the Decline of Serum Antibodies and Absence of Mucosal IgA. Cells. 2021; 10(10):2541. https://0-doi-org.brum.beds.ac.uk/10.3390/cells10102541
Chicago/Turabian StylePiano Mortari, Eva, Cristina Russo, Maria Rosaria Vinci, Sara Terreri, Ane Fernandez Salinas, Livia Piccioni, Claudia Alteri, Luna Colagrossi, Luana Coltella, Stefania Ranno, and et al. 2021. "Highly Specific Memory B Cells Generation after the 2nd Dose of BNT162b2 Vaccine Compensate for the Decline of Serum Antibodies and Absence of Mucosal IgA" Cells 10, no. 10: 2541. https://0-doi-org.brum.beds.ac.uk/10.3390/cells10102541