Genome-Wide Identification and Expression Profiling Analysis of the Trihelix Gene Family Under Abiotic Stresses in Medicago truncatula
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Treatments
2.2. Identification of the Trihelix Genes in M. truncatula
2.3. Chromosomal Distribution and Gene Duplication Events Analysis
2.4. Gene Structure and Conserved Motifs Analysis
2.5. Phylogenetic and Collinearity Analysis of MtGT genes
2.6. Tissue-Specific Expression and Abiotic Stress Expression Analysis
2.7. Expression Analysis of the MtGT Genes by Real-Time qPCR
3. Results
3.1. Identification of MtGT Genes in M. truncatula
3.2. Phylogenetic Analysis and Classification of MtGT Genes
3.3. Chromosomal Distribution and Gene Duplication Events of MtGT Family
3.4. Gene Structural Characteristics and Conserved Motifs Compositions of MtGT Genes
3.5. Evolutionary and Collinearity Analysis within MtGT Genes and Several Species
3.6. Expression Patterns of MtGT Genes in Different Tissues
3.7. Expression Profiling Analysis of MtGT Genes in Response to Abiotic Stress
3.8. Expression Profiling of MtGT Genes in Response to ABA Treatments
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Name | Gene ID | Length (aa) | Signature Domain | MW (KDa) | Subcellular Localization | pI | GRAVY |
---|---|---|---|---|---|---|---|
MtGT-1 | Medtr1g009220.1 | 319 | 19–104 | 37,270.73 | Nucleus | 5.36 | −1.039 |
MtGT-2 | Medtr1g037650.1 | 189 | 35–122 | 21,313.82 | Nucleus | 9.14 | −0.917 |
MtGT-3 | Medtr1g060990.1 | 511 | 129–217 | 58,779.54 | Nucleus | 6.57 | −1.173 |
MtGT-4 | Medtr1g061640.1 | 496 | 121–247 | 56,941.93 | Nucleus | 6.46 | −1.242 |
MtGT-5 | Medtr1g081180.1 | 557 | 101–181 | 64,373.39 | Nucleus | 5.71 | −1.104 |
MtGT-6 | Medtr1g492790.1 | 501 | 112–200 | 57,848.24 | Nucleus | 5.99 | −0.921 |
MtGT-7 | Medtr1g094045.1 | 381 | 53–183 | 42,978.72 | Extracell/Nucleus | 9.09 | −1.072 |
MtGT-8 | Medtr1g098870.1 | 565 | 53–140 | 64,378.61 | Nucleus | 5.91 | −1.088 |
MtGT-9 | Medtr1g098900.1 | 637 | 55–142 | 70,838.09 | Nucleus | 5.77 | −0.900 |
MtGT-10 | Medtr1g098920.1 | 450 | 123–250 | 51,386.17 | Nucleus | 6.16 | −1.049 |
MtGT-11 | Medtr1g112650.1 | 316 | 40–131 | 35,249.58 | Chloroplast/Nucleus | 4.97 | −0.653 |
MtGT-12 | Medtr2g016140.1 | 332 | 20–114 | 37,437.82 | Chloroplast | 5.37 | −0.703 |
MtGT-13 | Medtr2g016140.2 | 317 | 20–114 | 35,816.88 | Chloroplast/Nucleus | 5.29 | −0.805 |
MtGT-14 | Medtr2g026725.1 | 379 | 260–348 | 42,411.51 | Nucleus | 7.74 | −0.924 |
MtGT-15 | Medtr2g092960.1 | 1223 | 43–136 | 140,855.87 | Nucleus | 8.51 | −0.845 |
MtGT-16 | Medtr2g098080.1 | 445 | 112–238 | 50,604.51 | Nucleus | 6.21 | −0.991 |
MtGT-17 | Medtr2g102227.1 | 496 | 272–360 | 57,303.69 | Nucleus | 4.48 | −1.395 |
MtGT-18 | Medtr3g014280.1 | 325 | 35–122 | 37,084.54 | Nucleus | 9.03 | −1.057 |
MtGT-19 | Medtr3g089020.1 | 867 | 773–857 | 96,355.08 | Chloroplast | 7.97 | −0.400 |
MtGT-20 | Medtr3g085960.1 | 390 | 74–163 | 44,613.12 | Chloroplast/Nucleus/Peroxisome | 5.90 | −0.834 |
MtGT-21 | Medtr4g015680.1 | 279 | 40–125 | 33,950.09 | Nucleus | 6.55 | −1.283 |
MtGT-22 | Medtr4g079960.1 | 313 | 28–123 | 35,566.23 | Nucleus | 9.53 | −0.778 |
MtGT-23 | Medtr4g079960.2 | 304 | 28–123 | 34,456.87 | Nucleus | 9.60 | −0.818 |
MtGT-24 | Medtr4g079960.3 | 315 | 28–123 | 35,784.64 | Nucleus | 9.73 | −0.774 |
MtGT-25 | Medtr4g117990.1 | 340 | 41–135 | 37,889.76 | Nucleus | 9.07 | −0.915 |
MtGT-26 | Medtr5g017500.1 | 229 | 20–115 | 26,637.29 | Nucleus | 9.22 | −0.834 |
MtGT-27 | Medtr5g026540.1 | 302 | 43–128 | 35,678.99 | Nucleus | 8.34 | −1.208 |
MtGT-28 | Medtr6g035315.1 | 526 | 117–243 | 59,762.71 | Nucleus | 6.05 | −0.988 |
MtGT-29 | Medtr6g035370.1 | 646 | 53–140 | 72,115.32 | Nucleus | 5.74 | −1.018 |
MtGT-30 | Medtr6g486270.1 | 370 | 127–218 | 42,750.57 | Nucleus | 4.59 | −1.147 |
MtGT-31 | Medtr7g020870.1 | 341 | 38–130 | 37,377.48 | Nucleus | 5.32 | −0.740 |
MtGT-32 | Medtr7g020870.2 | 245 | 38–131 | 26,785.93 | Nucleus | 5.77 | −0.636 |
MtGT-33 | Medtr7g068770.1 | 293 | 51–136 | 35,338.67 | Nucleus | 6.62 | −1.346 |
MtGT-34 | Medtr7g081190.1 | 388 | 43–140 | 43,393.75 | Nucleus | 9.64 | −0.969 |
MtGT-35 | Medtr7g103390.1 | 455 | 101–227 | 52,293.03 | Nucleus | 6.02 | −1.204 |
MtGT-36 | Medtr7g114860.1 | 483 | 379–476 | 55,781.34 | Nucleus | 6.34 | −0.832 |
MtGT-37 | Medtr8g022290.1 | 371 | 65–161 | 40,319.8 | Nucleus | 9.53 | −0.803 |
MtGT-38 | Medtr8g100130.1 | 361 | 45–139 | 40,525.42 | Nucleus | 5.36 | −0.803 |
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Liu, X.; Zhang, H.; Ma, L.; Wang, Z.; Wang, K. Genome-Wide Identification and Expression Profiling Analysis of the Trihelix Gene Family Under Abiotic Stresses in Medicago truncatula. Genes 2020, 11, 1389. https://0-doi-org.brum.beds.ac.uk/10.3390/genes11111389
Liu X, Zhang H, Ma L, Wang Z, Wang K. Genome-Wide Identification and Expression Profiling Analysis of the Trihelix Gene Family Under Abiotic Stresses in Medicago truncatula. Genes. 2020; 11(11):1389. https://0-doi-org.brum.beds.ac.uk/10.3390/genes11111389
Chicago/Turabian StyleLiu, Xiqiang, Han Zhang, Lin Ma, Zan Wang, and Kun Wang. 2020. "Genome-Wide Identification and Expression Profiling Analysis of the Trihelix Gene Family Under Abiotic Stresses in Medicago truncatula" Genes 11, no. 11: 1389. https://0-doi-org.brum.beds.ac.uk/10.3390/genes11111389