Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials, Growth Conditions, and Stress Treatment
2.2. Cloning and Sequence Analysis of the LcSAIN3 Gene
2.3. qRT-PCR Analysis
2.4. Subcellular Localization of LcSAIN3
2.5. Construct Creation and Plant Genetic Transformation
2.6. Phenotypic Analysis of Transgenic Plants
2.7. Measurement of Proline Content and (Superoxide Dismutase) SOD Activity
2.8. Statistical Analysis
3. Results
3.1. Isolation and Sequence Analysis of LcSAIN3
3.2. Expression Analysis of LcSAIN3
3.3. Subcellular Localization of LcSAIN3
3.4. Overexpression of LcSAIN3 in Arabidopsis Improves Seed Germination under Salt Stress
3.5. LcSAIN3 Overexpression in Arabidopsis Enhanced Seed Germination under ABA and Osmotic Stress
3.6. LcSAIN3 Overexpression Enhances Tolerance to Salt Stress in Arabidopsis Seedlings
3.7. LcSAIN3 Regulates Proline Accumulation and SOD Activity in Response to Salt Stress
3.8. LcSAIN3 Overexpression Alters the Expression of Salt-Responsive Genes in Arabidopsis Plants
3.9. The Expression of LcSAIN3 in Sheepgrass Germplasms with Different Salt Tolerance
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Li, X.; Yang, W.; Jia, J.; Zhao, P.; Qi, D.; Chen, S.; Cheng, L.; Cheng, L.; Liu, G. Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis. Genes 2021, 12, 1994. https://0-doi-org.brum.beds.ac.uk/10.3390/genes12121994
Li X, Yang W, Jia J, Zhao P, Qi D, Chen S, Cheng L, Cheng L, Liu G. Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis. Genes. 2021; 12(12):1994. https://0-doi-org.brum.beds.ac.uk/10.3390/genes12121994
Chicago/Turabian StyleLi, Xiaoxia, Weiguang Yang, Junting Jia, Pincang Zhao, Dongmei Qi, Shuangyan Chen, Li Cheng, Liqin Cheng, and Gongshe Liu. 2021. "Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis" Genes 12, no. 12: 1994. https://0-doi-org.brum.beds.ac.uk/10.3390/genes12121994