Protective Role of Taurine on Rat Offspring Hypertension in the Setting of Maternal Chronic Kidney Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Model
2.2. Analysis of H2S-Generating Enzymes and RAAS Components using qPCR
2.3. Tissue H2S-Producing Capacity
2.4. Analysis of NO Parameters Using HPLC
2.5. 16S rRNA Sequencing
2.6. Statistics
3. Results
3.1. Body Weight and BP
3.2. H2S System in the Kidneys
3.3. NO Parameters
3.4. RAAS
3.5. Gut Microbiota Composition
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Gene Accession No | Forward | Reverse |
---|---|---|---|
Renin | J02941.1 | 5 aacattaccagggcaactttcact 3 | 5 acccccttcatggtgatctg 3 |
PRR | AB188298.1 | 5 gaggcagtgaccctcaacat 3 | 5 ccctcctcacacaacaaggt 3 |
AGT | XM_032887807.1 | 5 gcccaggtcgcgatgat 3 | 5 tgtacaagatgctgagtgaggcaa 3 |
ACE | U03734.1 | 5 caccggcaaggtctgctt 3 | 5 cttggcatagtttcgtgaggaa 3 |
AT1R | NM_030985.4 | 5 gctgggcaacgagtttgtct 3 | 5 cagtccttcagctggatcttca 3 |
CSE | NM_017074.2 | 5 cgcacaaattgtccacaaac 3 | 5 gctctgtccttctcaggcac 3 |
CBS | NM_012522.2 | 5 atgctgcagaaaggcttcat 3 | 5 gtggaaaccagtcggtgtct 3 |
DAO | NM_053626.1 | 5 ccctttctggaaaagcacag 3 | 5 ctcctctcaccacctcttcg 3 |
3MST | NM_138843.2 | 5 ggctcagtaaacatcccattc 3 | 5 tgtccttcacagggtcttcc 3 |
R18S | X01117 | 5 gccgcggtaattccagctcca 3 | 5 cccgcccgctcccaagatc 3 |
Groups | C | CKD | T | CKDT |
---|---|---|---|---|
Body weight, g | 280 ± 5 | 281 ± 9 | 308 ± 9 | 269 ± 9 *,# |
Left kidney weight, g | 1.27 ± 0.029 | 1.49 ± 0.057 * | 1.30 ± 0.032 # | 1.19 ± 0.034 *,# |
Left kidney weight/body weight | 0.046 ± 0.001 | 0.053 ± 0.001 * | 0.042 ± 0.001 # | 0.044 ± 0.002 # |
Creatinine, μM | 1.38 ± 0.51 | 1.29 ± 0.29 | 1.45 ± 0.52 | 1.37 ± 0.61 |
Systolic blood pressure, mmHg | 131 ± 1 | 148 ± 2 * | 131 ± 1 # | 132 ± 1 # |
Diastolic blood pressure, mmHg | 88 ± 1 | 101 ± 2 * | 86 ± 2 # | 91 ± 2 # |
Mean arterial pressure, mmHg | 102 ± 1 | 117 ± 2 * | 101 ± 2 # | 104 ± 2 # |
Groups | C | CKD | T | CKDT |
---|---|---|---|---|
L-citrulline, μM | 56.6 ± 4.1 | 50.9 ± 1.3 | 59.8 ± 2.4 | 58.5 ± 2.6 |
L-arginine, μM | 179.0 ± 6.3 | 161.6 ± 3.4 | 164.5 ± 9.1 | 166.3 ± 3.4 |
ADMA, μM | 1.71 ± 0.07 | 2.02 ± 0.09 | 1.85 ± 0.08 | 2.18 ± 0.06 |
SDMA, μM | 1.77 ± 0.07 | 1.62 ± 0.10 | 1.87 ± 0.04 | 2.03 ± 0.1 |
L-arginine to ADMA ratio, μM/μM | 113.7 ± 6.0 | 75.2 ± 4.1 | 95.6 ± 7.6 | 72.4 ± 1.8 |
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Tain, Y.-L.; Hou, C.-Y.; Chang-Chien, G.-P.; Lin, S.; Hsu, C.-N. Protective Role of Taurine on Rat Offspring Hypertension in the Setting of Maternal Chronic Kidney Disease. Antioxidants 2023, 12, 2059. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox12122059
Tain Y-L, Hou C-Y, Chang-Chien G-P, Lin S, Hsu C-N. Protective Role of Taurine on Rat Offspring Hypertension in the Setting of Maternal Chronic Kidney Disease. Antioxidants. 2023; 12(12):2059. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox12122059
Chicago/Turabian StyleTain, You-Lin, Chih-Yao Hou, Guo-Ping Chang-Chien, Sufan Lin, and Chien-Ning Hsu. 2023. "Protective Role of Taurine on Rat Offspring Hypertension in the Setting of Maternal Chronic Kidney Disease" Antioxidants 12, no. 12: 2059. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox12122059