Determination of the Empirical Electrokinetic Equilibrium Condition of Microorganisms in Microfluidic Devices
Abstract
:1. Introduction
2. Theory
3. Materials and Methods
3.1. Microfluidic Devices
3.2. Samples and Suspending Medium
3.3. Equipment and Software
3.4. Experimental Procedure
4. Results and Discussion
4.1. Discussion of Methods for Estimating in iEK Microfluidic Devices
4.2. Estimations of the New Parameter
4.3. Application of the New Parameter to Estimate Stable Trapping Voltages (sTVs)
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Microorganism Type | Species | Size (µm) | SD (%) | |
---|---|---|---|---|
Bacteria | Bacillus cereus (ATCC® 14579™) | Length: 4.94 ± 0.47 Width: 1.32 ± 0.13 | 618 ± 32 | 5.2 |
Escherichia coli (ATCC® 25922) | Length: 2.01 ± 0.42 Width: 0.97 ± 0.21 | 1092 ± 76 | 6.9 | |
Salmonella enterica (TT9079) | Length: 2.00 ± 0.31 Width: 0.97 ± 0.11 | 1928 ± 170 | 8.8 | |
Bacteriophages | 201Φ2-1 | Head dia.: 0.200 Tail: 0.020 × 0.211 | 3094 ± 238 | 7.7 |
ΦKZ | Head dia.: 0.145 Tail: 0.022 × 0.200 | 2140 ± 107 | 5.0 | |
SPN3US | Head dia.: 0.060 Tail: 0.018 × 0.035 | 2932 ± 246 | 8.4 |
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Coll De Peña, A.; Hill, N.; Lapizco-Encinas, B.H. Determination of the Empirical Electrokinetic Equilibrium Condition of Microorganisms in Microfluidic Devices. Biosensors 2020, 10, 148. https://0-doi-org.brum.beds.ac.uk/10.3390/bios10100148
Coll De Peña A, Hill N, Lapizco-Encinas BH. Determination of the Empirical Electrokinetic Equilibrium Condition of Microorganisms in Microfluidic Devices. Biosensors. 2020; 10(10):148. https://0-doi-org.brum.beds.ac.uk/10.3390/bios10100148
Chicago/Turabian StyleColl De Peña, Adriana, Nicole Hill, and Blanca H. Lapizco-Encinas. 2020. "Determination of the Empirical Electrokinetic Equilibrium Condition of Microorganisms in Microfluidic Devices" Biosensors 10, no. 10: 148. https://0-doi-org.brum.beds.ac.uk/10.3390/bios10100148