Synthesis of Antimicrobial Benzimidazole–Pyrazole Compounds and Their Biological Activities

Abstract

The synthesis of new compounds with antimicrobial and antiviral properties is a central objective today in the context of the COVID-19 pandemic. Benzimidazole and pyrazole compounds have remarkable biological properties, such as antimicrobial, antiviral, antitumor, analgesic, anti-inflammatory, anti-Alzheimer’s, antiulcer, antidiabetic. Moreover, recent literature mentions the syntheses and antimicrobial properties of some benzimidazole–pyrazole hybrids, as well as other biological properties thereof. In this review, we aim to review the methods of synthesis of these hybrids, the antimicrobial activities of the compounds, their correlation with various groups present on the molecule, as well as their pharmaceutical properties.

1. Introduction

Microbial resistance is one of the burning issues facing clinical practice and finding new effective compounds against multi-resistant pathogens is one of the major goals in current biomedical research [1]. The discovery of new antimicrobial and antiviral compounds is a major goal in the context of today’s COVID-19 pandemic [2]. It is known that both patients with severe cases and patients with moderate cases of COVID-19, with or without pneumonia, have received treatments with various antibiotics [3]. Among the heterocyclic compounds known in the literature for their antimicrobial activities are those with benzimidazole ring and those with pyrazole ring, aromatic compounds with a very wide range of medicinal properties. Benzimidazole derivatives developed a considerable interest in medical domain due to their therapeutic action as antitumor [4,5,6,7], antimicrobial [8,9,10,11,12,13,14], antihelmintic [15], antihistaminic [16,17], proton pump inhibitors [16,18], anti-inflammatory [19,20] and anti-hypertensive [21] drugs. Astemizole-related compounds demonstrated anti-prion activity for the treatment of Creutzfeldt–Jakob disease, while albendazole compounds are currently used as medication for the treatment of a variety of parasitic worm infestations. Additionally, benzimidazoles treat mitochondrial dysfunction in Alzheimer’s disease [22], possess neurotropic, psychoactive, analgesic effects [23], anticoagulant proprieties [24] and are efficient agents in Diabetes mellitus [25]. Additionally, pyrazole compounds possess a diversity of biological activities as analgesic [26,27,28], anticonvulsivant [29,30], antitumor [31,32,33,34], antidiabetic [35,36], antimicrobial [37,38,39,40,41,42,43], antipyretic [44,45], antiviral [46,47], antimalarial [48,49], local anesthetic [50] and so forth.

Moreover, the literature mentions a series of benzimidazole–pyrazole hybrids with remarkable antimicrobial properties, and not only, antiviral activities, even anti-COVID-19 [51,52,53,54], in the context of the new pandemic, which has led us to current research, to study their synthesis methods, antimicrobial properties, structure–property relationships, and their biological activities.

In this review, we aim to review the various methods of synthesis of benzimidazole–pyrazole hybrid compounds with antibacterial and antifungal properties, DNA-Gyrase inhibitors, topoisomerase IV inhibitors, as well as the other biological properties they possess, such as: antitumor, antioxidant, anti-inflammatory, analgesic, antiulcer (Figure 1). In order to highlight the structures of the heterocycles in the discussed compounds, we colored the benzimidazole nucleus with blue, the pyrazole with green, the linker with red, and the compounds with good biological activity are marked with a rectangle.

2. Synthesis, Antimicrobial Activities of Benzimidazole–Pyrazole Compounds. Benzimidazole–Pyrazole Compounds as Potent DNA Gyrase and Topoisomerase IV Inhibitors

2.1. Benzimidazoles Substituted in the “2” Position with Pyrazole Moiety

Benzimidazole chalcones 2a–2n, synthesized from 2-acetylbenzimidazole 1 and aldehydes in ethanolic KOH by a Claisen–Schmidt condensation, were cyclocondensated with izoniazide, to give (3-(1H-benzo[d]imidazol-2-yl)-5-(aryl)-4,5-dihydro-1H-pyrazol-1-yl)(pyridin-4-yl)methanones 3a–3n in good yields (Scheme 1). All compounds showed antimicrobial activity against bacterial strains E. coli, P. aeruginosa, S. aureus, S. pyogenes and fungi C. albicans, A. niger and A. clavatus. The compounds 3d, 3g, 3h, were found to be the best antibacterials, with MIC of 25 μg mL⁻¹ against P. aeuginosa (3d) and E. coli (3g, 3h) and compound 3n the best antifungal, with MIC 25 μg mL⁻¹ against A. niger [55].

Rajora and Srivastava reported the synthesis of some 2-(1H-pyrazol-3-yl)-1H-benzo[d]imidazoles by bromination of benzimidazolyl chalcone 4, with the formation of dibrominated intermediates 5a–5f, followed by cyclization in the presence of hydrazine hydrate and dehydrobromination, with the formation of compounds 6a–6f (Scheme 2).

Compounds 6a–6f showed good antimicrobial activity on four bacterial strains, E. coli, P. aeruginusa, B. subtilis, K. pneumoniae and two fungi, Candida albicons and Aspergillus niger, considering ciprofoxacin and fluconazole as standard drugs [56].

2-Chloro-1-(5-hydroxy-3-methyl-1-phenyl-1H-pyrazol-4-yl)ethanone 8 synthesized by refluxing 5-pyrazolone 7 with chloroacetyl chloride in a basic dioxane solution reacted with 2-aminobenzimidazole 9 to give N-(3-methyl-1-phenyl-1H-furo [2,3-c]pyrazol-4(5H)-ylidene)-1H-benzimidazol-2-amine 10 (Scheme 3) [57]. Compound 10 showed a very good anti-Gram-positive profile, being equivalent to chloramphenicol against B. subtilis (MIC 3.125 μg mL⁻¹), significant activity against B. thuringiensis (MIC 6.25 μg mL⁻¹) and also good antibacterial activities against Gram-positive bacteria, E. coli (MIC 50 μg mL⁻¹) and P. aeruginosa (MIC 50 μg mL⁻¹). Antifungal activity of the compound 10 was 50% lower than cycloheximide in inhibitory the growth of B. fabae and F. oxysporum (MIC 6.25 μg mL⁻¹).

A similar condensation of chalcones 11a–11k with intermediate hydrazide 12 in acetic acid, at 130 °C afforded new benzimidazole bearing pyrazoline derivatives 13a–13k in excellent yields (Scheme 4). All compounds showed antimicrobial activity against bacteria E. coli MTCC443, P. aeruginosa MTCC1688, S. aureus MTCC 96, S. pyogenes MTCC 442 and fungi C. albicans MTCC 227, A. niger MTCC 282 and A. clavatus MTCC 1323. Compounds 13a–13d showed the highest inhibition against almost all bacteria tested with values of minimum inhibitory concentrations of 25–50 mg mL⁻¹, while derivatives 13e–13k had antifungal activity against almost all strains tested, with similar CMI values [58]. Structure–activity relationship studies have shown that the presence of electron-withdrawing groups in the aromatic ring, like F, Cl, Br and NO₂, are responsible for increasing antimicrobial activity for most microorganisms tested.

Kalaria et al., reported a L-proline promoted one-pot four-component tandem reaction for synthesis of compound 16, starting from carbothioamide 14, pyrazolyl aldehyde 15, α-bromoethylacetate and malononitrile (Scheme 5) [59]. Antibacterial activity of the compounds 16 was screened against three Gram-positive bacteria (Streptococcus pneumoniae MTCC 1936, Bacillus subtilis MTCC 441 and Clostridium tetani MTCC 449) and three Gram-negative bacteria (Escherichia coli MTCC 443, Salmonella typhi MTCC 98, Vibrio cholerae MTCC 3906) using ampicillin, norfloxacin and ciprofloxacin as the standard antibacterial drugs. Compound 16 illustrated an excellent activity against Gram-positive bacteria B. subtilis (62.5 μg mL⁻¹), being more potent than ampicillin (250 μg mL⁻¹) and norfloxacin (100 μg mL⁻¹) and also against C. tetani, with a CMI of 200 μg mL⁻¹ compared with 250 μg mL⁻¹ for ampicilin. Additionally, the structure–activity relationship (SAR) showed that the presence of benzimidazole in the fifth position in the pyrazole ring is responsible for its biological activity.

Patil et al., reported two series o benzimidazole–pyrazole compounds 19a–19f and 20a–20f in two steps: a condensation between 2-benzimidazolehydrazine 17 and pyrazole 18a–18f, followed by cyclization with thioglicolic acid (Scheme 6) [60]. The compounds 19b, 19d, 20a and 20f show good activity against bacteria P. aeruginosa, S. aureus and P. vulgaris, while the others show moderate to poor activity against all pathogens. The compounds 19a and 19c exhibited good activity against fungal strains A. niger and A. flavus.

Reddy et al., reported the synthesis of a new class of pyrazolyl–benzimidazoles 23a–23c possessing an amide group by reaction between pyrazolones 21a–21c with 1H-benzo[d]imidazol-2-amine 9, and the oxidation of the intermediate compounds 22a–22c with chloranil (Scheme 7) [61]. It was found that the presence of electron-withdrawing substituent “Cl” on the aromatic ring increases the antimicrobial activity, compound 23c being a potent antifungal agent against A. niger considering ketoconazole as standard. Additionally, compounds 23a and 23c possess antimicrobial activity against B. subtilis and P. aeruginosa (chloramphenicol standard).

Padalkar et al., synthesized a new class of antimicrobial agents, by reaction of phenyl hydrazine with substituted acetophenones 24 to give the corresponding hydrazones 25, which on Vilsmeier–Haack reaction with POCl₃–DMF gave substituted 3-aryl-4-formyl pyrazoles 26. Compounds 25a–25b were condensed with o-substituted aromatic amines 27 in the presence of PCl₃ in ethanol to obtain corresponding 2-[substituted-1H-pyrazol-4-yl]-1H-benzimidazoles 28b–28i (Scheme 8) [62]. The compound 28g showed good antibacterial activity against Escherichia coli and Staphylococcus aureus, and compounds 28d, 28e, 28h exhibited weak to moderate growth inhibitory activity against both E. coli and S. aureus as revealed from their MIC values (

Table 1. Antibacterial and antifungal activities of the compounds 28a–28h indicated by MIC (μg mL⁻¹).

Compound	E. coli	S. aureus	C. albicans	A. niger
28b	312	312	312	312
28c	187.5	312	312	312
28d	250	62.5	250	187.5
28e	187.5	62.5	312	250
28f	312	187.5	62.5	312
28g	62.5	62.5	312	312
28h	125	62.5	62.5	125
Streptomycin	125	125	-	-
Fluconazole	-	-	125	125

). Compounds 28f and 28h show good inhibitory growth in the case of Candida albicans (MIC = 62.5 μg mL⁻¹). Saundane et al., reported the synthesis of a series of benzimidazole–pyrazole compounds using a two-step strategy (Scheme 9): synthesis of intermediate chalcones 25a–25b by a condensation reaction, followed by a cyclization reaction with hydazine (compounds 31) or phenylhydrazine (compounds 32) [63]. All compounds were assessed for their in vitro antibacterial activity against four representative bacterial species E. coli (MTCC-723), S. aureus (ATCC-29513), K. pneumonia (NCTC-13368) and P. aeruginosa (MTCC-1688) using gentamycin as a reference and for their antifungal activity against A. oryzae (MTCC-3567T), A. niger (MTCC-281), A. flavus (MTCC-1973), A. terreus (MTCC-1782). Compounds 31a and 32a possess good antibacterial and antifungal activity (

Table 2. Antibacterial and antifungal activities of the compounds 28a–28h.

Compound	Antibacterial Activity (MIC μg mL−1)				Antifungal Activity (MIC μg mL−1)
	Ec	Sa	Kp	Pa	Ao	An	Af	At
31a	8	8	16	16	16	8	16	8
31b	32	64	128	128	64	128	256	128
31c	128	512	512	256	512	512	256	128
32a	8	16	16	32	32	16	8	32
32b	32	32	16	128	128	128	256	128
32c	32	64	512	256	128	256	256	128
Gentamycin	2	2	2	2	-	-	-	-
Fluconazole	-	-	-	-	2	2	2	2

Ec: Escherichia coli (MTCC-723), Sa: Staphylococcus aureus (ATCC-29513), Kp: Klebsiella pneumonia (NCTC-13368), Pa: Pseudomonas aeruginosa (MTCC-1688), Ao: Aspergillus oryzae (MTCC-3567T), An: Aspergillus niger (MTCC-281), Af: Aspergillus flavus (MTCC-1973), At: Aspergillus terreus (MTCC-1782).

), against E. coli, S. aureus (MIC = 8 μg mL⁻¹) and A. niger (MIC = 8 μg mL⁻¹ for 31a). Additionally, all compounds possess antioxidant activity.

Padhy et al., synthesized two series of benzimidazole–pyrazole compounds in three steps: (i) Claisen–Schmidt condensation of 2-acetylbezimidazole 1 with substituted aromatic aldehydes in presence of NaOH, to give the intermediates chalcones 33a–33e; (ii) condensation of the chalcones 33 with benzyl chloride gave the corresponding 1-benzyl substituted compounds 34a–34e; (iii) the reaction of compounds 34 with phenylhydrazine in the presence of acetic acid afforded 1-benzyl-2-(5-aryl-1-phenyl-4,5-dihydro-1H-pyrazol-3-yl)-1H-benzimidazoles 35a–35e, while (iv) condensation with thiosemicarbazide in presence of NaOH, give 5-aryl-3-(1-benzyl-1H-benzimidazol-2-yl)-4,5-dihydro-1H-pyrazole-1-carbothioamides 36a–36e in good yields (Scheme 10). The in vitro antimicrobial activity of compounds 35–36 was tested against four bacterial strains, S. aureus, B. subtilis, E. coli, P. aeruginosa, and one fungus, C. albicans. The compounds exhibited weaker antimicrobial activities compared to those of the control drugs (Ciprofloxacin and Fluconazole), the MIC values of the compounds ranged between 64–1024 μg mL^–1 for the 1-phenylpyrazolines 35a–35e and between 128–512 μg mL^–1 for the pyrazoline-1-carbothioamides 36a–36e. Compound 35e showed good activity (64 μg mL^–1) against all tested bacterial strains [64].

4-(1H-benzimidazol-2-yl)benzenamine 37, obtained by cyclization reaction of 1,2-phenylenediamine with 4-amino benzoic acid, was diazotized and treated with ethylacetoacetate to produce ethyl 2-(2-(4-(1H-benzimidazol-2-yl)phenyl) hydrazono)-3-oxobutanoate 38 through intramolecular rearrangement reaction. Dehydrative cyclisation of 38 in the with different hydrazine hydrochlorides produce corresponding benzimidazole–pyrazole 39a–39i (Scheme 11) [65]. The antitubercular and antimicrobial activity of compounds 39 was determined on four Gram-positive strains, three Gram-negative strains and 2 fungi. In

Table 3. Minimum inhibitory concentration (μg mL⁻¹) of the compounds 39a–39i.

Compound	Antitubercular Activity	Antibacterial Activity							Antifungal Activity
		Gram-Positive Bacteria				Gram-Negative Bacteria
	Mt	Sa	Se	Ml	Bc	Ec	Pa	Kp	An	Af
39a	125.5	62.5	62.5	125	62.5	62.5	125	31.25	62.5	31.25
39b	62.5	31.25	31.25	62.5	31.25	62.5	62.5	31.25	31.25	15.62
39c	3.9	7.81	1.95	3.9	7.81	7.81	7.81	1.95	3.9	1.95
39d	7.81	7.81	3.9	7.81	7.81	7.81	7.81	3.9	7.81	3.9
39e	62.5	62.5	31.25	62.5	62.5	62.5	62.5	31.25	62.5	31.25
39f	3.9	125	3.9	3.9	7.81	7.81	3.9	1.95	3.9	1.95
39g	7.81	15.62	3.9	7.81	7.81	15.62	7.81	3.9	7.81	3.9
39h	>125	125	125	>125	>125	125	>125	62.5	62.5	62.5
39i	125	125	125	>125	125	62.5	125	62.5	62.5	31.25
Standard	0.97	125	1.95	3.9	7.81	7.81	3.9	1.95	7.81	3.9

Mb: Mycobacterium tuberculosis, Sa: Staphylococcus aureus ATCC 9144, Se: Staphylococcus epidermidis ATCC 155, Ml: Micrococcus luteus ATCC 4698, Bacillus cereus ATCC 11778, Ec: Escherichia coli ATCC 25922, Pa: Pseudomonas aeruginosa ATCC 2853, Kp: Klebsiella pneumoniae ATCC 11298, An: Aspergillus niger ATCC 9029, Af: Aspergillus fumigatus ATCC 46645. Standard: Isoniazid: reference standard against M. tuberculosis, Ciprofloxacin: standard for other bacteria, Ketoconazol: reference standard for fungi.

we marked in green the very good antimicrobial activities of compounds 39c and 39f, as well as of the compounds 3d and 3g, for the labeled strains, and the very good antibacterial activities of all compounds against Staphylococcus aureus. The values of the minimum inhibitory concentrations (MIC) in Table 3 showed that compounds, 39c and 39f, possess almost all MICs as good as the standards used for antitubercular and antimicrobial activities, and their antifungal activities are twice as high, compared to Ketoconazole, ie 3.9 μg mL^–1 against Aspergillus niger ATCC 9029 and 1.95 μg mL^–1 against Aspergillus fumigatus ATCC 46645. With the exception of compounds 39f, 39h and 39i, all compounds showed better antibacterial activity (MIC = 125 μg mL^–1) than standard, Ciprofloxacin, compounds 39c and 39d being 16 times more active(MIC = 7.81 μg mL^–1) than standard.

Suram et al., reported the synthesis of a series of bis(benzimidazolyl)pyrazole compounds from chlororacetylpyrazole-benzimidazole 40 and benzimidazoles 41–44, to obtain compounds 45–48 (Scheme 12) [66]. It was observed that the compound with thio ethanone linkage 45 and amino ethanone linkage 47 displayed slightly higher activity than that with methyl thio ethanone 46 and methyl amino ethanone linkage 48 on the microbial tested strains, S. aureus, B. subtilis, P. aeruginosa, K. pneumoniae, A. niger and P. chrysogenum, when compared with the standard drugs chloramphenicol and ketoconazole.

A new class of benzimidazole–pyrazoles was prepared using a Claisen–Schmidt reaction [67]. From all synthesized compounds, derivative 51, obtained by cyclocondensation reaction of thioamide 49 with 4-fluorophenacyl bromide 50 (Scheme 13), having nitro substituent on the aromatic ring showed greater antimicrobial activity particularly against Pseudomonas aeruginosa, with an inhibition zone of 34 mm at 100 μg per well, and Penicillium chrysogenum, with an inhibition zone of 41 mm at 100 μg per well.

Si et al., synthesized two series of benzimidazole–pyrazoles by reaction of benzimidazole 52 with pyrazole-5-carbonyl chlorides 53 and 54 to afford the final compounds 55a–55f and 56a–56f (Scheme 14) [68]. The authors reported the antifungal activities against four fungi, B. cinerea, R. solani, F. graminearum, A. solani, considering hymexazol as the positive control at 100 μg mL ⁻¹ (

Table 4. Inhibitory rates of the compounds 55–56 against four phytopathogenic fungi at 100 ug mL⁻¹.

Compound	B. cinerea	R. solani	F. graminearum	A. solani
55a	65.53	63.34	39.21	29.71
55b	83.11	64.84	52.37	34.57
55c	72.37	62.34	51.38	52.57
55d	79.68	69.08	52.63	58.28
55e	85.62	63.34	45.52	6.00
55f	85.39	62.84	47.37	68.28
56a	43.38	38.65	21.05	21.42
56b	45.66	30.17	18.42	29.14
56c	44.98	39.90	22.36	24.57
56d	52.28	36.66	25.79	34.00
56e	52.28	34.91	17.36	62.57
56f	41.55	45.38	25.00	30.85
Hymexazol	100.00	72.82	68.16	55.43

). All compounds showed better inhibitory activity against B. cinerea. The inhibition rates of compounds 55a–55f exceeded 60% against R. solani and the inhibition rates of compounds 55a–55f ranged from 58.28% to 68.28% against A. solani, which were better than 55.43% of the control hymexazol. The compounds with pyrazole-4-carboxamide moiety 55a–55f showed higher activities than the target compounds with pyrazole-5-carboxamide moiety 56a–56f. Thus, the activities of 55a and 55b were better than those of 56a and 56b and the activities of 55c and 55d were better than those of 56c and 56d.

Jardosh et al., synthesized new pyrido[1,2-a]benzimidazoles starting chloroformilation and alkilation of 4-methyl-2-p-tolylcyclopent-3-enone 57. In the next step, a one-pot three-component reaction was used to afford final compounds 61a–61c (Scheme 15). The in vitro antimicrobial activity of 61a–61c against S. typhi, S. pneumoniae, E. coli, C. tetani, V. cholera, B. subtilis, C. albicans and A. fumigatus using broth microdilution technique was assessed. All compounds 61a–61c displayed good antimicrobial activity compared to standard drugs, as can be seen in

Table 5. In vitro antimicrobial activity of benzimidazole–pyrazoles 61a–61c.

Compounds	Microorganisms (μg mL−1)
	B. subtilis	C. tetanis	S. pneumoniae	E. coli	S.typhi	V. cholera	A. fumigatus	C. albicans
61a	100	200	100	200	250	250	>1000	250
61b	500	200	200	250	250	50	200	>1000
61c	250	250	250	62.5	200	100	>1000	250
Ciprofloxacin	50	100	50	25	25	25	-	-
Chloramphenicol	50	50	50	50	50	50	-	-
Norfloxacin	100	50	10	10	10	10	-	-
Ampicillin	250	250	100	100	100	100	-	-
Griseofulvin	-	-	-	--	-	-	100	500

[69].

Sowdari et al., synthesized a new class of diamidomethane-linked benzazolyl–pyrazoles 64a–64c by a green approach, using the synthesis strategy indicated in Scheme 16 [70]. Compounds 64a and 64c were found to be potential antifungal agents against Aspergillus niger (MIC = 50 and 25 μg mL⁻¹, respectively) and Penicillium chrysogenum (MIC = 12.5 and 12.5 μg mL⁻¹, respectively) compared to the standard drug, Ketoconazole.

β-ketoacyl-acyl carrier protein synthase III (FabH) is an attractive target for the development of new antibacterial agents, because it catalyzes the initial step of fatty acid biosynthesis, essential for bacterial survival. Thus, Wang et al., reported the synthesis of a new series of benzimidazole–pyrazol amides with low toxicity and potent FabH inhibitory. Synthesis of compound 67 from 1-(4-fluorophenyl)ethanone is accomplished in four steps: condensation with phenylhydrazine, followed by cyclization by reflux with POCl₃ in DMF for 5 h, to obtain pyrazole 65, which with 1,2-phenylenediamine and Na₂S₂O₅ has provided benzimidazole–pyrazole intermediate 66, which by acylation with nicotinic acid, DMAP and EDC hydrochloride led to the final product 67 (Scheme 17). Compound 67 showed the most potent inhibition activity against four bacteria strains (with MIC of 0.98, 0.49, 0.98, 0.98 μg mL⁻¹, respectively, against E. coli, P. aeruginosa, B. subtilis and S. aureus) and FabH (with IC₅₀ of 1.22 μM). Additionally, FabH mutant Xanthomonas Campestris experiment validated that compounds binding site outcomes FabH.

2D molecular docking modeling and surrounding residues of E. coli FabH was also performed for compound 67 (Figure 2) [71].

A special method for the synthesis of a benzimidazolo–pyrazole compound was reported by Chkirate et al., [72]. Thus, condensation of 1,2-phenylenediamine with dehydroacetic acid afford 68 which reacted with 1-bromobutane to give the alkylated 1,5-benzodiazepine 69. Compound 69 reacts with an excess of hydrazine monohydrate to afford the pyrazolyl–benzimidazole 70 (Scheme 18). The minimum inhibitory concentration (MIC) of 70 against S. aureus, E. coli and P. aeruginosa was evaluated at 12.5 μg mL⁻¹, 50 μg mL⁻¹ and 50 μg mL⁻¹, respectively, compared to standard drug Chloramphenicol. Additionally, Co(II) and Zn(II) complexes of 70 possess remarkable antibacterial activity.

Elaziz et al., synthesized benzimidazole–pyrazole 74 from 1-methylbenzimdazole 71 and diazonium salt 72, through the intermediate 73 [73]. Compound 74 possessed better antibacterial activity than standard Cephalothin against anaerobic E. coli (16.5 μg mL⁻¹ versus 24.3 μg mL⁻¹), Salmonella typhimurium (13.4 μg mL⁻¹ versus 28.5 μg mL⁻¹), and better antibacterial activity than standard Chloramphenicol against Bacillus subtilis (23.3 μg mL⁻¹ versus 32.4 μg mL⁻¹, Scheme 19,

Table 6. Antibacterial activity of the compound 74 and the standards (MIC μg mL⁻¹).

Compound	Staphylococcus aureus	Bacillus subtilis	Escherichia coli anaerobic	Salmonella typhimurium
74	25.3	23.3	16.5	13.4
Chloramphenicol	24.5	32.4	-	-
Cephalothin	-	-	24.3	28.5

).

Bassyouni et al., synthesized three series of benzimidazole–pyrazoles 76–78b [74]. Compounds 76a and 76b were synthesized by the reaction of 75a and 75b with ethyl cyanoacetate in ethanol in the presence of triethylamine, respectively (Scheme 20). Methylation of 76a and 76b was achieved by their reaction with methyl iodide or DMC that yielded compounds 77a and 77b. Compounds 77a and 77b reacted with 4-aminoantipyrine in ethanol, in the presence of catalytic amounts of acetic acid to give 78a and 78b. The antibacterial activity of the compounds 76a, 76b, 77b, 78a and 78b was examined with Gram-positive bacteria Bacillus subtilis, Bacillus cereus and Staphylococcus aureus, Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium. The antibacterial activity showed that compound 76a was the most active against S. typhimurium and its activity exceeded the activity of the reference antibiotic amoxicillin. Compounds 77b and 78b exhibited high antimicrobial activity against S. aureus (

Table 7. The antimicrobial activity of the compounds 76a, 76b, 77b, 78a, 78b.

Microorganism	Inhibition Zone Diameter (mm/mg Sample)
	76a	76b	77b	78a	78b	Amoxicillin
Bacillus cereus	10	7	9	7	10	22
Bacillus subtilis	-	-	10	7	9	25
Staphylococcus aureus	10	10	18	10	18	16
Escherichia coli	7	12	8	8	-	22
Pseudomonas aeruginosa	10	9	11	11	13	30
Salmonella typhimurium	40	-	-	-	11	20

).

Benzimidazolo–pyrazole compounds 80a–80h and 81a–81h were synthesized from the reaction of chalcones 79a–79h with phenylhydrazine and 2,4-dinitrophenylhydrazine, respectively (Scheme 21) [75]. All compounds were screened for their antimicrobial activities against E. coli, P. aeruginosa, S. aureus, B. subtilis, C. albicans and A. niger (

Table 8. The antimicrobial activity of the compounds 80a–80h and 81a–81h.

Compounds	Zone of Inhibition at 100 μg mL−1 (in mm)
	E. coli	P. aeruginosa	S. aureus	B. subtilis	C. albicans	A. niger
80a	-	-	-	15	-	-
80b	14	13	14	16	-	-
80d	12	17	-	-	-	-
80e	-	12	12	-	-	-
80f	-	-	14	18	-	-
80g	13	-	-	-	-	-
80h	19	16	18	15	-	-
81b	-	-	-	-	13	16
81c	-	-	14	13	-	-
81d	17	15	-	-	18	-
81f	-	-	-	12	14	17
81g	-	13	15	-	-	-
81h	-	-	-	-	-	14
Gentamycin	22	22	19	20	-	-
Ketoconazole	-	-	-	-	25	20

). The best antimicrobial activities of the compounds are marked in green in Table 8. It is observed that compounds 80b and 80h showed a good antibacterial activity against all the strains tested, and compounds with 2,4-dinitrophenylhydrazine had better antifungal activity than the antibacterial one, e.g., compounds 81b and 81f. Only compound 80a showed significant antitubercular activity at the concentration of 100μg mL⁻¹ compared with the standard drug, Rifampicin.

El-Gohary et al., synthesized benzimidazole–pyrazole molecules 83a–83b with antimicrobial properties, using the reaction between benzimidazoles 82a–82b and 3-methyl-1H-pyrazol-5(4H)-one in dimethylformamide (DMF), in presence of triethyl-amine (TEA) as catalyst (Scheme 22) [76]. The compounds 83a–83b showed very good antimicrobial activity against two bacteria B. cereus and S. aureus, against two fungi, C. albicans and A. fumigatus, compared to the standards used, ampicillin and fluconazole (

Table 9. Antibacterial and antifungal activities of compounds 83a–83b.

Compounds	MIC, μg mL−1 (mM)
	B. cereus	S. aureus	C. albicans	A. fumigatus
83a	1250 (5.48)	156.25 (0.684)	625 (2.74)	312.5 (1.37)
83b	1250 (4.57)	2500 (9.15)	1250 (4.57)	625 (2.29)
Ampicillin	1250 (3.58)	312.5 (0.894)	-	-
Fluconazole	-	-	2500 (8.16)	-

).

The benzimidazole–pyrazole 85 synthesized by cyclization of benzimidazole 84 in the reaction with of ethyl 3-oxobutanoate (Scheme 23), possessed good antifungal activity, against C. albicans (MIC = 2500 μg mL⁻¹) compared with standard Fluconazole (MIC = 2500 μg mL⁻¹) [77].

2.2. Benzimidazoles Substituted in the Position “1” with Pyrazole Moiety

Krishnanjaneyulu et al., synthesized a series of 1-substituted benzimidazoles with pyrazole moiety through a linker using a four-step strategy: benzimidazole synthesis, N-alkylation, condensation with aldehydes with the formation of chalcones 87a–87i and cyclization with the formation of the pyrazole nucleus, in compounds 88a–88i (Scheme 24) [78]. All compounds were evaluated for their antibacterial activity against four Gram-positive bacteria: Staphylococcus aureus ATCC 9144, Staphylococcus epidermidis ATCC 155, Micrococcus luteus ATCC 4698 and Bacillus cereus ATCC 11778, and three Gram-negative bacteria: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 2853 and Klebsiella pneumoniae ATCC 11298. The antifungal activity of the compounds 88a–88i was evaluated against two fungi, Aspergillus niger ATCC 9029 and Aspergillus fumigatus ATCC 46645 (

Table 10. Minimum inhibitory concentration in μg mL⁻¹ of the compounds 88a–88l.

Compounds	Sa	Se	Ml	Bc	Ec	Pa	Kp	An	Af
88a	31.25	31.25	15.62	62.5	31.25	31.25	15.62	62.5	31.25
88b	125	62.5	31.25	125	62.5	125	31.25	125	125
88c	125	62.5	125	125	62.5	62.5	31.25	125	125
88d	62.5	31.25	31.25	31.25	62.5	62.5	31.25	125	62.5
88e	15.62	15.62	7.81	15.62	15.62	7.81	7.81	31.25	15.62
88f	62.5	31.25	31.25	31.25	62.5	125	31.25	125	62.5
88g	15.62	7.81	7.81	7.81	15.62	7.81	7.81	31.25	7.81
88h	31.25	15.62	15.62	15.62	15.62	15.62	7.81	31.25	15.62
88i	15.62	7.81	3.9	7.81	7.81	15.62	7.81	15.62	7.81
88j	31.25	31.25	15.62	31.25	31.25	62.5	15.62	125	31.25
88k	62.5	62.5	31.25	31.25	62.5	125	31.25	125	125
88l	31.25	15.62	15.62	15.62	31.25	15.62	7.81	31.25	15.62
Ciprofloxacin	15.62	7.81	7.81	7.81	15.62	7.81	3.9	-	-
Ketoconazole	-	-	-	-	-	-	-	15.62	7.81

Sa: Staphylococcus aureus ATCC 9144; Se: Staphylococcus epidermidis ATCC 155; Ml: Micrococcus luteus ATCC 4698; Bc: Bacillus cereus ATCC 11778; Ec: Escherichia coli ATCC 25922; Pa: Pseudomonas aeruginosa ATCC 2853; Kp: Klebsiella pneumoniae ATCC 11298, An: Aspergillus niger ATCC 9029; Af: Aspergillus fumigatus ATCC 46645.

). It was found that compounds containing an electron-withdrawing group at the phenyl group attached to C-5 of pyrazole displayed superior antimicrobial activities than compounds possessing an electron-releasing group. The unsubstituted derivatives displayed moderate antimicrobial activity. The best antimicrobial activity was shown by compounds 88g and 88i, which in Table 10 is marked in green.

Dawoud et al., reported the synthesis of a benzimidazole–pyrazole compound 90 from chalcone intermediate 89 (Scheme 25). It was found by the agar diffusion method that compound 90 possessed good antimicrobial activity against Escherichia coli, Salmonella. SP., Staphylococcus aurous and Candida albicans [79].

Tumosienė et al., reported the synthesis of the benzimidazole–pyrazole compounds 94a–94b, in three steps, from 2-substituted benzimidazoles 91a–91b (Scheme 26). It was found that compound 94b shows good antimicrobial activity against Staphylococcus aureus ATCC 9144 and Escherichia coli ATCC 8739 (250 μg mL⁻¹) [80].

2.3. Benzimidazoles Substituted in the Position “4” (“7”) with Pyrazole Moiety

Grilot et al., reported the synthesis of second-generation antibacterial benzimidazole–pyrazoles from 2,3,6-trifluorobenzenamine in seven steps, as can be seen in Scheme 27 [81]. Compound 98a, with a 3-pyridine moiety at C5, which maintains a hydrogen bond with Arg136, showed a reasonable MIC against S. aureus (0.25 μg mL⁻¹). It can be observed that introduction of a fluorine atom at C6 on pyrazole 98b, has no improved antibacterial potency (0.25 μg mL⁻¹ against S. aureus), nor affinity for Gyrase B and Topoisomerase IV, as previously reported [80], but oral exposure was improved 2-fold, which led to the exclusive focus on C6-fluorobenzimidazole pyrazoles. The authors also studied the variation of MIC and the polarity of molecules with the introduction of the substituent in the “5” position, as seen in

Table 11. MIC values and SAR studies in pyrazole series 17a–17i.

Compounds				Minimum Inhibitory Concentration μg/mL
	C5	C6	C7′	Sa	Sa + HS	E fs	E fm	S p
98a		H	H	0.25	4	0.063	-	0.016
98b		F	H	0.25	2	0.063	-	0.032
98c		F	H	0.25	1	0.125	0.5	0.016
98d		F	Me	0.063	0.5	0.063	0.25	0.016
98e		F	Me	0.016	0.125	0.016	0.032	< 0.008
98f		F	H	0.125	3	0.125	0.5	0.032
98g		F	H	0.063	2	0.125	0.25	0.032
98h		F	H	0.25	4	0.063	0.25	0.016
98i		F	H	0.063	1	0.063	0.5	0.063

Sa = S. aureus; Sa + HS = S. aureus + 50% human serum; E fs = E. faecalis; E fm = E. faecium; S p = S. pneumoniae.

. Compound 98f showed a MIC against S. aureus of 0.125 µg mL⁻¹ and could be improved slightly by the addition of a methyl group on the C5 substituent, as in 98g and its resolution yielded compounds 98h and 98i. The (S)-isomer 98i was 4-fold more potent than the (R)-isomer 98h against S. aureus and showed acceptable oral exposure, but compound 98h exhibited a high serum shift (16-fold).

Charifson et al., reported the synthesis of benzimidazole–pyrazoles 101–102 from 5-bromo-2-nitro-3-(1H-pyrazol-1-yl)benzenamine 99, using a Suzuki coupling reaction (Scheme 28) [82]. The compounds were found to be inhibitors of DNA Gyrase and Topoisomerase IV, with potent antibacterial activity. The results of the evaluation for enzymatic inhibition and antibacterial potency of the compounds are shown in

Table 12. Gyrase and Topoisomerase IV Inhibition and antibacterial activities of the compounds.

Compound	Enzyme Inhibiton Data Ki (μM)			Minimum Inhibitory Concentration (μg/mL)
	S. aureus gyrase	E. coli gyrase	E. coli topoIV	S. aureus	S.pneumoniae	H. influenzae
101	0.015	<0.004	0.046	0.063	0.008	1
102	0.016	<0.004	0.058	0.031	<0.008	0.25

. The superior enzymatic and antibacterial inhibitory activity of compound 102 vs. 101 is observed, due to the presence of the pyrimidine nucleus in the molecule.

3. Biological Activities of Antimicrobial Benzimidazole–Pyrazole Compounds

3.1. Analgesic and Anti-Inflammatory Activity of Antimicrobial Benzimidazole–Pyrazole Compounds

Benzimidazole and pyrazole/pyrazoline are important nitrogen-containing heterocyclics in anti-inflammatory research [83,84]. In recent decades, there have been various studies on the anti-inflammatory activity of benzimidazole compounds.

Chikkula et al., reported the analgesic activity [65] of the compounds 39a–39i (Scheme 11). The analgesic activity of novel benzimidazole derivatives 39a–39i varied with reaction time. The compounds displayed moderate analgesic activity at 30 min of reaction time. In the second hour, the analgesic activity reached to peak level. Additionally, the presence of phenyl ring at the N-1 atom of pyrazole ring significantly increased analgesic activity. The anti-inflammatory activity of the synthesized compounds varies similarly to their analgesic activity. Compounds 39c, 39d and 39e had the best analgesic activity.

Arora et al., reported the synthesis of the compounds 104a–104e from chalcones 103a–103e (Scheme 29) [85]. The in vivo studies for analgesic activity were evaluated in albino mice by Eddy’s hot plate method. The compound 104a showed mild activity at a dose of 100 and 200 mg kg⁻¹ in the range of 7.38 ± 0.12–7.36 ± 0.15 at 90 min when compared with standard drug Diclofenac sodium at a dose of 5 mg kg⁻¹ in the range of 9.45 ± 0.28 at 90 min. Additionally, the compound 104a showed slightly less moderate activity in the range of 53.03 and 59.09% at a dose of 100 and 200 mg kg⁻¹ at a time interval of 3h of carrageenan challenge when compared to compound 104c (63.63%) as well as when compared with standard Diclofenac sodium at the same time period exhibited 69% of activity, respectively, at a dose of 100 mg kg⁻¹.

3.2. Benzimidazole–Pyrazole Compounds with Antitumor Activities

Kalirajan et al., [75] reported anticancer activity of compounds 80a–80h and 81a–81h against MCF7 human breast cell line by in vitro Sulforhodamine B assay (SRB assay) method. The compounds 80b, 81a and 81b have significant activity when compared with standard drug Doxorubicin (Adriamycin, ADR), with GI₅₀ values of 16.3 μg mL⁻¹, 16.0 μg mL⁻¹, and 17.1 μg mL⁻¹, respectively (Doxorubicin with GI₅₀ value <10 μg mL⁻¹). El-Gohary and Shaaban [76] reported the antitumor activity of compounds 83a and 83b and the results are shown in

Table 13. In vitro antitumor activity of the compounds 83a–83b toward Hep-G2, HCT-116 and MCF-7 cancer cell lines.

Compound	IC50 (mM)
	HepG2	HCT-116	MCF-7
83a	0.054	0.042	0.062
83b	0.265	0.20	0.281
5-Fluorouracil	0.061	0.041	0.0415

. Compound 83a, exhibiting the highest in vitro antitumor activity, was evaluated for in vivo antitumor activity against EAC in mice. Additionally, compound 83a was assessed for in vitro cytotoxicity toward human normal lung fibroblast (W138) cell line employing MTT assay [86,87,88] and utilizing 5-fluorouracil as a standard cytotoxic drug. IC₅₀ value for 83a was determined 0.246 mM, therefore less cytotoxic than 5-fluorouracil (IC₅₀ = 0.051 mM). Additionally, results of the DNA-binding assay confirmed that antimicrobial and antitumor compound 83a exerts its biological activities through interaction with DNA. Bezimidazole–pyrazole 85 displayed eminent activity toward HCT-116 cell line [77] with IC₅₀ value of 5.41 μM close to that of 5-fluorouracil of 4.00 μM and lower than that measured for the previously prepared benzimidazoles [76,89].

Wang et al., synthesized a series of benzimidazole grafted benzsulfamide-containing pyrazole ring derivatives using a strategy in two steps: 1. synthesis of benzimidazole–pyrazole hybrid by reaction of pirazolecarbaldehyde 105 with 1,2,-phenylenediamine and 2. reaction with sulfonyl chloride 107 with the hybrid 106 (Scheme 30) [90]. Compound 108 showed the most excellent inhibition against tubulin assembly (IC₅₀ = 1.52 μM) and in vitro growth inhibitory activity against a panel of four human cancer cell lines, IC₅₀ = 0.15, 0.21, 0.33 and 0.17 μM, respectively, for A549, Hela, HepG2 and MCF-7. Additionally, compound 108 validly induces A549 cell apoptosis, causes cell cycle arrest in the G2/M phase and disrupts the cellular microtubule network. Due to these promising results, along with molecular docking data, compound 108 is a potential anticancer agent.

Shake et al., reported synthesis of 1-substituted benzimidazoles with pyrazole moiety 109a–109d (Figure 3), by cyclization of the corresponding chalcones with hydrazine hydrate in ethanol, and their antitumor and antiviral activities [91]. The in vitro cytotoxic screening of the compounds 109a–109d against four different cell lines is showed in

Table 14. In vitro antitumor activity of the compounds 109a–109d toward A-549, HCT-116, Hep-G2 and MCF-7 cancer cell lines.

Compounds	% Inhibitory Activity against Tumor Cell Lines at 300 μM
	A-549	HCT-116	Hep-G2	MCF-7
109a	7.2	81.1	40.0	35.1
109b	38.3	65.5	60.8	49.4
109c	0.0	35.3	41.7	39.5
109d	48.8	49.9	62.4	59.6
Doxorubicin	84.1 ± 12.6	111.7 ± 20.5	63.6 ± 9.4	163.8 ± 10.1

. It can be seen that compound 109d has the best antitumor activity on all determined tumor lines.

3.3. Benzimidazole–Pyrazole Compounds as Antioxidants

Saundane et al., evaluated the scavenging effects of the compounds 31a–31c and 32a–32c [63] on the DPPH radical by Hatano’s method [92]. The RSA (Radical Scavenging Activity) results suggested that the compound 31a exhibited good antioxidant activity of 71.95 and 72.43 % at the concentration of 100 μg mL⁻¹. Additionally, the reductive ability of synthesized compounds was assessed by the extent of conversion of Fe^3+/ferricyanide complex to the Fe^2+/ferrous form. The reductive ability results suggested that the compound 31a exhibited good reducing power ability at the concentration of 100 μg mL⁻¹.

Bassyouni et al., reported that compounds 77a, 77b and 78b displayed mild antioxidant activity, of 227.9 μmol L⁻¹, 412.7 μmol L⁻¹ and 361.8 μmol L⁻¹, respectively, using the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay [74].

Durgamma et al., reported the synthesis of amido-linked benzimidazolyl–pyrazoles 110a–110c (Figure 4) from the corresponding chalcones [93]. All compounds possess good antioxidant activity in the DPPH method, with IC₅₀ values of 0.121 μmol mL⁻¹, 0.127 μmol mL⁻¹ and 0.135 μmol mL⁻¹, respectively, compared to ascorbic acid with IC₅₀ = 0.256 μmol mL⁻¹.

3.4. Benzimidazole–Pyrazole Compounds as Antiulcer Agents

Noor et al., synthesized the benzimidazole–pyrazole hybrids 113a–113f by reaction of benzimidazole–hydrazide 111 with chalcones 112a–112f in acetic acid (Scheme 31) [94]. The antiulcer activity of all the benzimidazole–pyrazole hybrids was tested in vivo by an ethanol-induced gastric ulcer model in rats, with Omeprazole (30 mg/kg) as a standard. All compounds 113a–113f exhibited an antiulcer effect.

4. Conclusions

This review summarizes the syntheses of benimidazole–pyrazole compounds with antimicrobial properties, as well as their biological activities mentioned in the literature. From the data presented, it can be concluded that hybrids with pyrazole moiety in position “4” (“7”) possess the strongest antimicrobial properties. The presence of certain groups grafted on the benzimidazole and pyrazole nuclei, such as -COOCH₃, -NHCO, -CHO, -CF₃, -NO₂, -CN, -F, -Cl, -OH, OCH₃, -N(CH₃)₂ as well as other heterocycles in the molecule (pyridine, pyrimidine, thiazole, indole), increases the antimicrobial activity of the compounds [95]. Additionally, the binding linker between benzimidazole and pyrazole is important for their antimicrobial activity. Additionally, the antimicrobial activity is improved if the molecule contains linker groups such as carbonyl (CO), amide (NHCO), or other heteroatoms. We hope that this review will be a starting point for the synthesis of other benzimidazole–pyrazole hybrids with antimicrobial properties, which have much better bacterial and antifungal properties than those of antibiotics marketed or used in hospitals today.