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Article

Optical DNA Mapping of Plasmids Reveals Clonal Spread of Carbapenem-Resistant Klebsiella pneumoniae in a Large Thai Hospital

1
Department of Biology and Biological Engineering, Chalmers University of Technology, 412 96 Gothenburg, Sweden
2
Department of Laboratory Medicine, Karolinska Institute, 141 52 Stockholm, Sweden
3
Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
4
Department of Clinical Microbiology, Karolinska University Hospital, 171 76 Stockholm, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Hanne Ingmer
Received: 22 June 2021 / Revised: 16 August 2021 / Accepted: 20 August 2021 / Published: 24 August 2021
(This article belongs to the Special Issue Solutions to Antimicrobial Resistance)
Carbapenem-resistant Klebsiella pneumoniae (CR-KP) in patients admitted to hospitals pose a great challenge to treatment. The genes causing resistance to carbapenems are mostly found in plasmids, mobile genetic elements that can spread easily to other bacterial strains, thus exacerbating the problem. Here, we studied 27 CR-KP isolates collected from different types of samples from 16 patients admitted to the medical ward at Siriraj Hospital in Bangkok, Thailand, using next generation sequencing (NGS) and optical DNA mapping (ODM). The majority of the isolates belonged to sequence type (ST) 16 and are described in detail herein. Using ODM, we identified the plasmid carrying the blaNDM-1 gene in the ST16 isolates and the plasmids were very similar, highlighting the possibility of using ODM of plasmids as a surrogate marker of nosocomial spread of bacteria. We also demonstrated that ODM could identify that the blaCTX-M-15 and blaOXA-232 genes in the ST16 isolates were encoded on separate plasmids from the blaNDM-1 gene and from each other. The other three isolates belonged to ST147 and each of them had distinct plasmids encoding blaNDM-1. View Full-Text
Keywords: optical DNA mapping; plasmids; carbapenem resistance; blaNDM-1; nosocomial transmission optical DNA mapping; plasmids; carbapenem resistance; blaNDM-1; nosocomial transmission
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MDPI and ACS Style

KK, S.; Sewunet, T.; Wangchinda, W.; Tangkoskul, T.; Thamlikitkul, V.; Giske, C.G.; Westerlund, F. Optical DNA Mapping of Plasmids Reveals Clonal Spread of Carbapenem-Resistant Klebsiella pneumoniae in a Large Thai Hospital. Antibiotics 2021, 10, 1029. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10091029

AMA Style

KK S, Sewunet T, Wangchinda W, Tangkoskul T, Thamlikitkul V, Giske CG, Westerlund F. Optical DNA Mapping of Plasmids Reveals Clonal Spread of Carbapenem-Resistant Klebsiella pneumoniae in a Large Thai Hospital. Antibiotics. 2021; 10(9):1029. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10091029

Chicago/Turabian Style

KK, Sriram, Tsegaye Sewunet, Walaiporn Wangchinda, Teerawit Tangkoskul, Visanu Thamlikitkul, Christian G. Giske, and Fredrik Westerlund. 2021. "Optical DNA Mapping of Plasmids Reveals Clonal Spread of Carbapenem-Resistant Klebsiella pneumoniae in a Large Thai Hospital" Antibiotics 10, no. 9: 1029. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10091029

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