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Article

Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography

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Department of Analytical Chemistry, Faculty of Chemistry, Universitat de València, 46100 Burjassot, Spain
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Laboratory of Clinical Analysis, General University Hospital of de Castelló, 12004 Castelló, Spain
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Department of Chemistry, Doctor Harisingh Gour Vishwavidyalaya (A Central University), Madhya Pradesh 470003, India
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Bioanalytical Chemistrya, Department of Physical and Analytical Chemistrya, ESTCE, Universitat Jaume I, 12071 Castelló, Spain
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Department of Criminology and Forensic Science, Doctor Harisingh Gour Vishwavidyalaya (A Central University), Madhya Pradesh 470003, India
*
Author to whom correspondence should be addressed.
Received: 22 October 2019 / Revised: 5 November 2019 / Accepted: 12 November 2019 / Published: 15 November 2019
(This article belongs to the Section Antibiotics Use and Antimicrobial Stewardship)
A method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column and a mobile phase of 0.05 M sodium dodecyl sulfate—7.5% 1-propanol—0.5% triethylamine, buffered at pH 3 with phosphate salt, running under the isocratic mode. The signal was monitored by fluorescence. Validation was successfully performed according to the EU Commission Decision 2002/657/EC in terms of specificity, calibration range (LOQ to 1 mg/kg), linearity (R2 > 0.9991), limit of detection and decision limit (0.01–0.05 mg/kg), limit of quantification (0.025–0.150 mg/kg), detection capability (<0.4 times decision limit), trueness (−14.2% to +9.8%), precision (<14.0%), robustness, and stability. The procedure was environmentally friendly, safe, easy-to-conduct, inexpensive, and had a high sample throughput, thus it is useful for routine analysis as a screening method in a laboratory for food residue control. View Full-Text
Keywords: food safety; fluorescence; laying hen; optimization; solid-to-liquid extraction; validation food safety; fluorescence; laying hen; optimization; solid-to-liquid extraction; validation
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MDPI and ACS Style

Peris-Vicente, J.; García-Ferrer, D.; Mishra, P.; Albiol-Chiva, J.; Durgbanshi, A.; Carda-Broch, S.; Bose, D.; Esteve-Romero, J. Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography. Antibiotics 2019, 8, 226. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8040226

AMA Style

Peris-Vicente J, García-Ferrer D, Mishra P, Albiol-Chiva J, Durgbanshi A, Carda-Broch S, Bose D, Esteve-Romero J. Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography. Antibiotics. 2019; 8(4):226. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8040226

Chicago/Turabian Style

Peris-Vicente, Juan, Daniel García-Ferrer, Pooja Mishra, Jaume Albiol-Chiva, Abhilasha Durgbanshi, Samuel Carda-Broch, Devasish Bose, and Josep Esteve-Romero. 2019. "Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography" Antibiotics 8, no. 4: 226. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8040226

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