Next Article in Journal
Short-Term Lincomycin Exposure Depletion of Murine Microbiota Affects Short-Chain Fatty Acids and Intestinal Morphology and Immunity
Next Article in Special Issue
A Putative Amidase Endolysin Encoded by Clostridium perfringens St13 Exhibits Specific Lytic Activity and Synergizes with the Muramidase Endolysin Psm
Previous Article in Journal
Use of Oral Tetracyclines in the Treatment of Adult Patients with Community-Acquired Bacterial Pneumonia: A Literature Review on the Often-Overlooked Antibiotic Class
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Antibiotics
Volume 9
Issue 12
10.3390/antibiotics9120906
Review Report
antibiotics-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Simultaneous Control of Staphylococcus aureus and Bacillus cereus Using a Hybrid Endolysin LysB4EAD-LysSA11

Antibiotics 2020, 9(12), 906; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9120906
by Bokyung Son 1,2, Minsuk Kong 3, Yoyeon Cha 1,2, Jaewoo Bai 4 and Sangryeol Ryu 1,2,5,6,*
Reviewer 1: Anonymous
Reviewer 2:
Hongping Wei
Antibiotics 2020, 9(12), 906; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9120906
Submission received: 24 November 2020 / Revised: 8 December 2020 / Accepted: 11 December 2020 / Published: 14 December 2020
(This article belongs to the Special Issue Development of Bacteriophage Derived Lysins and Depolymerases for Therapeutic Purposes in Combating Bacterial Pathogens)

Round 1

Reviewer 1 Report

The paper presents a very interesting approach to novel endolysin development, as antibacterial agents. The work is scientifically sound and simply and clearly presented.

As an optimization, for increasing the value of the article I would recommend  that the authors provide some form of graphical abstract, along with the written abstract. 

Also, concerning the results of the determinations of lytic activity at different temperatures and pH values, and antimicrobial activity in food samples it would be nice if the authors could produce a figure/table that briefly summarizes the comparative results at a glance. 

I therefore, consider this paper fit for publication in this journal.  

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

The manuscript by B. Son et al reported construction of chimeric lysins from LysB4 and LysSA11 and their activities against S. aureus and B. cereus. The results are interesting and provide new routes for engineering lysins. However, the authors need to explain what could be the advantages and the disadvantages of fusing two lysins into one protein, compare with mixing two separate lysins? Other points are:

  1. Line 79: Please clarify S. aureus lysins consisit of three domains, not two domains? 
  2. Line 89-90: "...a C-terminally truncated version of endolysins LysSA11EAD.." should be "..a N-terminally truncated version of endolysins LysSA11EAD.."?
  3. Figure 5: It is better to give the results of decontamination using both lysins LysSA11 and LysB4 together. 

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Back to TopTop