Cytological Study of Cypripedium japonicum Thunb. (Orchidaceae Juss.): An Endangered Species from Korea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cytological Analysis
2.2. Genome Size Measurement
3. Results and Discussion
3.1. Chromosome Numbers and Karyotype Structure of Cypripedium japonicum Thunb.
3.2. Genome Size Estimation in Korean C. japonicum
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Collection Number (Population_Individual) | Locality; Collector | Chromosome Number | Genome Size 1C ± S.D. (pg) |
---|---|---|---|
DY1_1 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | 2n = 22 | 29.58 ± 0.59 |
DY1_2 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | - | 29.27 ± 0.49 |
DY1_3 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | - | 29.59 ± 0.35 |
DY1_4 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | - | 29.86 ± 0.23 |
DY1_5 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | 2n = 22 | 29.99 ± 0.49 |
DY1_6 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | 2n = 22 | 29.97 ± 0.05 |
DY2_1 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | - | 29.47 ± 0.09 |
DY2_2 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | 2n = 22 | 28.77 ± 0.65 |
DY2_3 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | 2n = 22 | 28.39 ± 0.33 |
DY2_4 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | 2n = 22 | 29.43 ± 0.39 |
DY2_5 | Mt. Deokyu, Muju-gun, Jeollabuk-do; MHH | - | 29.94 ± 0.85 |
DY3_1 | Mt. Deokyu, Muju-gun, Jeollabuk-do; TSJ | 2n = 22 | 29.31 ± 0.44 |
YD_1 | Youngdong-gun, Chungcheongbuk-do; MHH | 2n = 22 | 29.20 ± 0.98 |
YD_2 | Youngdong-gun, Chungcheongbuk-do; MHH | 2n = 22 | 28.38 ± 0.38 |
YD_3 | Youngdong-gun, Chungcheongbuk-do; MHH | - | 29.48 ± 0.49 |
YD_4 | Youngdong-gun, Chungcheongbuk-do; MHH | - | 28.26 ± 0.45 |
YD_5 | Youngdong-gun, Chungcheongbuk-do; MHH | - | 30.14 ± 0.11 |
HC_1 | Hwacheon-gun, Gangwon-do; MHH | 2n = 22 | 29.29 ± 0.68 |
HC_2 | Hwacheon-gun, Gangwon-do; MHH | 2n = 22 | 28.73 ± 0.31 |
HC_3 | Hwacheon-gun, Gangwon-do; MHH | - | 29.59 ± 0.33 |
Taxon | 2n | References | 1C (pg) | References |
---|---|---|---|---|
Cypripedium acaule Aiton | 20 | [11] | 44.84 | [24] |
C. arietinum R.Br. | 20 | [11] | - | - |
C. calceolus L. | 20 | [11] | 32.35 | [24] |
41.05 | [29] | |||
C. californicum A.Gray | 20 | [30] | 21.60 | [30] |
C. candidum Muhl. ex Willd. | 20 | [11] | - | - |
C. cordigerum D.Don | 20 | [31] | - | - |
C. debile Rchb. | 20 | [32] | - | - |
C. elegans Rchb. | 20 | [11] | - | - |
C. fasciculatum kellogg | 20 | [11] | - | - |
C. flavum P.F.Hunt and Summerh. | 20 | [11] | 36.41 | [33] |
C. formosanum Hayata | 20, 30 | [10] | 28.50, 32.00 | [24] |
C. guttatum Sw. | 20 | [11] | - | - |
C. henryi Rolfe | 22 | [32] | 38.80 | [24] |
C. himalaicum Rolfe | 20, 30 | [11] | - | - |
C. japonicum Thunb. (population in Japan) | 20 | [10] | - | - |
C. japonicum Thunb. (population in Korea and China) | 22 | [12], Present study | 28.38–30.14 | Present study |
C. macranthos Sw. | 20, 22 | [11] | 37.40 | [24] |
C. molle Lindl. | 20 | [11] | 4.14 | [24] |
C. parviflorum Salisb. | 20 | [11] | - | - |
C. parviflorum var. makasin (Farw.) Sheviak | 20 | [11] | - | - |
C. parviflorum var. pubescens (Willd.) O. W. Knight | 20 | [30] | 32.40 | [30] |
C. passerinum Richardson | 20 | [11] | - | - |
C. plectrochilum Franch. | 20 | [11] | - | - |
C. reginae Walter | 20 | [11] | - | - |
C. segawae Masam. | 20 | [10] | - | - |
C. shanxiense S.C.Chen | 20 | [11] | - | - |
C. tibeticum King ex Rolfe | 20 | [32] | - | - |
C. yatabeanum Makino | 20 | [10] | - | - |
Collection Number (Population_Individual) | Absolute Chromosome Length (µm) | HCL1 ± S.D. (µm) | AsI 2 (%) | RI 3 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | ||||
DY1_1 | 14.57 | 13.49 | 12.47 | 9.94 | 9.81 | 9.31 | 8.67 | 7.71 | 6.27 | 5.25 | 4.58 | 102.07 ± 3.27 | 66.7 | 1.69 |
DY1_5 | 12.33 | 10.26 | 9.38 | 9.00 | 8.25 | 7.86 | 7.30 | 7.11 | 6.47 | 4.94 | 4.26 | 87.04 ± 2.30 | 59.9 | 1.84 |
DY2_2 | 12.52 | 10.69 | 9.28 | 9.43 | 8.27 | 6.78 | 8.56 | 7.31 | 6.22 | 5.38 | 4.72 | 89.16 ± 2.33 | 61.9 | 3.51 |
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Choi, B.; Gang, G.-H.; Kim, H.; Byun, H.; Kwak, M.; So, S.; Myeong, H.-H.; Jang, T.-S. Cytological Study of Cypripedium japonicum Thunb. (Orchidaceae Juss.): An Endangered Species from Korea. Plants 2021, 10, 1978. https://0-doi-org.brum.beds.ac.uk/10.3390/plants10101978
Choi B, Gang G-H, Kim H, Byun H, Kwak M, So S, Myeong H-H, Jang T-S. Cytological Study of Cypripedium japonicum Thunb. (Orchidaceae Juss.): An Endangered Species from Korea. Plants. 2021; 10(10):1978. https://0-doi-org.brum.beds.ac.uk/10.3390/plants10101978
Chicago/Turabian StyleChoi, Bokyung, Geun-Hye Gang, Hyeonjin Kim, Hyejoo Byun, Minyeong Kwak, Soonku So, Hyeon-Ho Myeong, and Tae-Soo Jang. 2021. "Cytological Study of Cypripedium japonicum Thunb. (Orchidaceae Juss.): An Endangered Species from Korea" Plants 10, no. 10: 1978. https://0-doi-org.brum.beds.ac.uk/10.3390/plants10101978