Genome-Wide Analysis of ZAT Gene Family in Osmanthus fragrans and the Function Exploration of OfZAT35 in Cold Stress
Abstract
:1. Introduction
2. Results
2.1. Identification, Distribution, Duplication, and Physicochemical Characteristics of OfZATs
2.2. Phylogenetic Analysis of ZAT Genes
2.3. Structure and Motif Composition of OfZATs
2.4. Cis-Elements Analysis in OfZATs
2.5. Expression Pattern of OfZAT Genes in Different O. fragrans Tissues
2.6. Expression Patterns of OfZATs under Salt Stress
2.7. Expression Patterns of OfZATs under Cold Stress
2.8. Subcellular Localization and Transcriptional Activation Activity
2.9. Analysis of Physiological Parameters
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Identification, Phylogenesis, Chromosomal Localization, Synteny, and Physicochemical Analysis of OfZATs
4.3. Gene Structure, Conserved Motif Compositions, and Cis-Element Analysis
4.4. Expression Profiles of OfZATs in Tissues under Abiotic Stress
4.5. RNA Extraction and qRT-PCR
4.6. Subcellular Localization and Transcriptional Activation Activity of OfZAT35
4.7. Analyses of Physiological Parameters
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Ding, H.; Yang, Z.; Zai, Z.; Feng, K.; Wang, L.; Yue, Y.; Yang, X. Genome-Wide Analysis of ZAT Gene Family in Osmanthus fragrans and the Function Exploration of OfZAT35 in Cold Stress. Plants 2023, 12, 2346. https://0-doi-org.brum.beds.ac.uk/10.3390/plants12122346
Ding H, Yang Z, Zai Z, Feng K, Wang L, Yue Y, Yang X. Genome-Wide Analysis of ZAT Gene Family in Osmanthus fragrans and the Function Exploration of OfZAT35 in Cold Stress. Plants. 2023; 12(12):2346. https://0-doi-org.brum.beds.ac.uk/10.3390/plants12122346
Chicago/Turabian StyleDing, Huifen, Zhandong Yang, Zhouying Zai, Keyi Feng, Lianggui Wang, Yuanzheng Yue, and Xiulian Yang. 2023. "Genome-Wide Analysis of ZAT Gene Family in Osmanthus fragrans and the Function Exploration of OfZAT35 in Cold Stress" Plants 12, no. 12: 2346. https://0-doi-org.brum.beds.ac.uk/10.3390/plants12122346