Reflectance Spectroscopy for the Classification and Prediction of Pigments in Agronomic Crops
Abstract
:1. Introduction
2. Results
2.1. Descriptive and Variance Analysis-Based Attributes of Crops
2.2. Hyperspectral Analysis in Leaves
2.3. Cluster Heatmap of Selected Wavelengths and Classification-Based UV–VIS–NIR–SWIR Bands
2.4. Principal Component Analysis (PCA), Correlation Coefficients, and Loadings of the Wavelengths
2.5. Machine Learning and Artificial Intelligence Algorithms for Classification
2.6. Calibration, Cross-Validation, and Prediction Simultaneous Models by Crop Leaf-Based Partial Least Squares Regression
2.7. Vegetation Indices and Pigment Profiling
3. Discussion
3.1. Remote Sensing Sensor and Pigment Phenotyping in Leaves for High-Throughput Monitoring Crops
3.2. Artificial Intelligence Algorithms Improvement Selection Pigment in Crops
3.3. Quantitative and Optimization PLSR Models to Estimate Pigments in Crops
3.4. Vegetation Indices Combined for Pigment Phenotyping
4. Material and Methods
4.1. Plant Materials
4.2. Pigment Quantifications and Hyperspectral Analysis
4.3. Statistical Analyses
4.3.1. Analysis of Variance and Descriptive Statistics
4.3.2. Analysis of Leaf Reflectance Spectral Fingerprints
4.3.3. Machine Learning, Artificial Algorithms, and Hyperspectral Vegetation Index
4.3.4. Vegetation Indices
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Boshkovski, B.; Doupis, G.; Zapolska, A.; Kalaitzidis, C.; Koubouris, G. Hyperspectral Imagery Detects Water Deficit and Salinity Effects on Photosynthesis and Antioxidant Enzyme Activity of Three Greek Olive Varieties. Sustainability 2022, 14, 1432. [Google Scholar] [CrossRef]
- Zhang, N.; Zhou, X.; Kang, M.; Hu, B.-G.; Heuvelink, E.; Marcelis, L.F.M. Machine Learning versus Crop Growth Models: An Ally, Not a Rival. AoB Plants 2022, 15, plac061. [Google Scholar] [CrossRef] [PubMed]
- El-Hendawy, S.; Al-Suhaibani, N.; Mubushar, M.; Tahir, M.U.; Marey, S.; Refay, Y.; Tola, E. Combining Hyperspectral Reflectance and Multivariate Regression Models to Estimate Plant Biomass of Advanced Spring Wheat Lines in Diverse Phenological Stages under Salinity Conditions. Appl. Sci. 2022, 12, 1983. [Google Scholar] [CrossRef]
- Fu, Y.; Yang, G.; Song, X.; Li, Z.; Xu, X.; Feng, H.; Zhao, C. Improved Estimation of Winter Wheat Aboveground Biomass Using Multiscale Textures Extracted from UAV-Based Digital Images and Hyperspectral Feature Analysis. Remote Sens. 2021, 13, 581. [Google Scholar] [CrossRef]
- Yoosefzadeh-Najafabadi, M.; Tulpan, D.; Eskandari, M. Using Hybrid Artificial Intelligence and Evolutionary Optimization Algorithms for Estimating Soybean Yield and Fresh Biomass Using Hyperspectral Vegetation Indices. Remote Sens. 2021, 13, 2555. [Google Scholar] [CrossRef]
- Li, K.-Y.; de Lima, R.; Burnside, N.G.; Vahtmäe, E.; Kutser, T.; Sepp, K.; Cabral Pinheiro, V.H.; Yang, M.-D.; Vain, A.; Sepp, K. Toward Automated Machine Learning-Based Hyperspectral Image Analysis in Crop Yield and Biomass Estimation. Remote Sens. 2022, 14, 1114. [Google Scholar] [CrossRef]
- Wang, D.; Cao, W.; Zhang, F.; Li, Z.; Xu, S.; Wu, X. A Review of Deep Learning in Multiscale Agricultural Sensing. Remote Sens. 2022, 14, 559. [Google Scholar] [CrossRef]
- Mao, Y.; Li, H.; Wang, Y.; Fan, K.; Song, Y.; Han, X.; Zhang, J.; Ding, S.; Song, D.; Wang, H.; et al. Prediction of Tea Polyphenols, Free Amino Acids and Caffeine Content in Tea Leaves during Wilting and Fermentation Using Hyperspectral Imaging. Foods 2022, 11, 2537. [Google Scholar] [CrossRef]
- SharathKumar, M.; Heuvelink, E.; Marcelis, L.F.M. Vertical Farming: Moving from Genetic to Environmental Modification. Trends Plant Sci. 2020, 25, 724–727. [Google Scholar] [CrossRef]
- Clemente, A.A.; Maciel, G.M.; Siquieroli, A.C.S.; de Araujo Gallis, R.B.; Pereira, L.M.; Duarte, J.G. High-Throughput Phenotyping to Detect Anthocyanins, Chlorophylls, and Carotenoids in Red Lettuce Germplasm. Int. J. Appl. Earth Obs. Geoinf. 2021, 103, 102533. [Google Scholar] [CrossRef]
- Cotrozzi, L.; Lorenzini, G.; Nali, C.; Pellegrini, E.; Saponaro, V.; Hoshika, Y.; Arab, L.; Rennenberg, H.; Paoletti, E. Hyperspectral Reflectance of Light-Adapted Leaves Can Predict Both Dark- and Light-Adapted Chl Fluorescence Parameters, and the Effects of Chronic Ozone Exposure on Date Palm (Phoenix dactylifera). Int. J. Mol. Sci. 2020, 21, 6441. [Google Scholar] [CrossRef]
- El-Sharkawy, M.; Sheta, A.; El-Wahed, M.; Arafat, S.; Behiery, O. Precision Agriculture Using Remote Sensing and GIS for Peanut Crop Production in Arid Land. Int. J. Plant Soil Sci. 2016, 10, 1–9. [Google Scholar] [CrossRef]
- Falcioni, R.; Gonçalves, J.V.F.; de Oliveira, K.M.; Antunes, W.C.; Nanni, M.R. VIS-NIR-SWIR Hyperspectroscopy Combined with Data Mining and Machine Learning for Classification of Predicted Chemometrics of Green Lettuce. Remote Sens. 2022, 14, 6330. [Google Scholar] [CrossRef]
- Falcioni, R.; Gonçalves, J.V.F.; de Oliveira, K.M.; de Oliveira, C.A.; Demattê, J.A.M.; Antunes, W.C.; Nanni, M.R. Enhancing Pigment Phenotyping and Classification in Lettuce through the Integration of Reflectance Spectroscopy and AI Algorithms. Plants 2023, 12, 1333. [Google Scholar] [CrossRef]
- Falcioni, R.; Antunes, W.C.; Demattê, J.A.M.; Nanni, M.R. Biophysical, Biochemical, and Photochemical Analyses Using Reflectance Hyperspectroscopy and Chlorophyll a Fluorescence Kinetics in Variegated Leaves. Biology 2023, 12, 704. [Google Scholar] [CrossRef]
- Crusiol, L.G.T.; Sun, L.; Sun, Z.; Chen, R.; Wu, Y.; Ma, J.; Song, C. In-Season Monitoring of Maize Leaf Water Content Using Ground-Based and UAV-Based Hyperspectral Data. Sustainability 2022, 14, 9039. [Google Scholar] [CrossRef]
- Fernandes, A.M.; Fortini, E.A.; Müller, L.A.D.C.; Batista, D.S.; Vieira, L.M.; Silva, P.O.; do Amaral, C.H.; Poethig, R.S.; Otoni, W.C. Leaf Development Stages and Ontogenetic Changes in Passionfruit (Passiflora edulis Sims.) Are Detected by Narrowband Spectral Signal. J. Photochem. Photobiol. B Biol. 2020, 209, 111931. [Google Scholar] [CrossRef]
- Silva, C.A.; Nanni, M.R.; Teodoro, P.E.; Silva, G.F.C. Vegetation Indices for Discrimination of Soybean Areas: A New Approach. Agron. J. 2017, 109, 1331–1343. [Google Scholar] [CrossRef]
- Wang, Y.; Ma, H.; Wang, J.; Liu, L.; Pietikäinen, M.; Zhang, Z.; Chen, X. Hyperspectral Monitor of Soil Chromium Contaminant Based on Deep Learning Network Model in the Eastern Junggar Coalfield. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 2021, 257, 119739. [Google Scholar] [CrossRef]
- Hassanzadeh, A.; Murphy, S.P.; Pethybridge, S.J.; van Aardt, J. Growth Stage Classification and Harvest Scheduling of Snap Bean Using Hyperspectral Sensing: A Greenhouse Study. Remote Sens. 2020, 12, 3809. [Google Scholar] [CrossRef]
- Feng, L.; Zhang, Z.; Ma, Y.; Du, Q.; Williams, P.; Drewry, J.; Luck, B. Alfalfa Yield Prediction Using UAV-Based Hyperspectral Imagery and Ensemble Learning. Remote Sens. 2020, 12, 2028. [Google Scholar] [CrossRef]
- Ropelewska, E. Application of Imaging and Artificial Intelligence for Quality Monitoring of Stored Black Currant (Ribes nigrum L.). Foods 2022, 11, 3589. [Google Scholar] [CrossRef] [PubMed]
- Crusiol, L.G.T.; Nanni, M.R.; Furlanetto, R.H.; Sibaldelli, R.N.R.; Sun, L.; Gonçalves, S.L.; Foloni, J.S.S.; Mertz-Henning, L.M.; Nepomuceno, A.L.; Neumaier, N.; et al. Assessing the Sensitive Spectral Bands for Soybean Water Status Monitoring and Soil Moisture Prediction Using Leaf-Based Hyperspectral Reflectance. Agric. Water Manag. 2023, 277, 108089. [Google Scholar] [CrossRef]
- Phuangsaijai, N.; Theanjumpol, P.; Kittiwachana, S. Performance Optimization of a Developed Near-Infrared Spectrometer Using Calibration Transfer with a Variety of Transfer Samples for Geographical Origin Identification of Coffee Beans. Molecules 2022, 27, 8208. [Google Scholar] [CrossRef]
- Wang, H.; Mortensen, A.K.; Mao, P.; Boelt, B.; Gislum, R. Estimating the Nitrogen Nutrition Index in Grass Seed Crops Using a UAV-Mounted Multispectral Camera. Int. J. Remote Sens. 2019, 40, 2467–2482. [Google Scholar] [CrossRef]
- Falcioni, R.; Moriwaki, T.; Gibin, M.S.; Vollmann, A.; Pattaro, M.C.; Giacomelli, M.E.; Sato, F.; Nanni, M.R.; Antunes, W.C. Classification and Prediction by Pigment Content in Lettuce (Lactuca sativa L.) Varieties Using Machine Learning and ATR-FTIR Spectroscopy. Plants 2022, 11, 3413. [Google Scholar] [CrossRef]
- Izenman, A.J. Modern Multivariate Statistical Techniques, 1st ed.; Springer: Berlin/Heidelberg, Germany, 2008. [Google Scholar]
- Franca, T.; Goncalves, D.; Cena, C. ATR-FTIR Spectroscopy Combined with Machine Learning for Classification of PVA/PVP Blends in Low Concentration. Vib. Spectrosc. 2022, 120, 103378. [Google Scholar] [CrossRef]
- Braga, P.; Crusiol, L.G.T.; Nanni, M.R.; Caranhato, A.L.H.; Fuhrmann, M.B.; Nepomuceno, A.L.; Neumaier, N.; Farias, J.R.B.; Koltun, A.; Gonçalves, L.S.A.; et al. Vegetation Indices and NIR-SWIR Spectral Bands as a Phenotyping Tool for Water Status Determination in Soybean. Precis. Agric. 2021, 22, 249–266. [Google Scholar] [CrossRef]
- Sobejano-Paz, V.; Mikkelsen, T.N.; Baum, A.; Mo, X.; Liu, S.; Köppl, C.J.; Johnson, M.S.; Gulyas, L.; García, M. Hyperspectral and Thermal Sensing of Stomatal Conductance, Transpiration, and Photosynthesis for Soybean and Maize under Drought. Remote Sens. 2020, 12, 3182. [Google Scholar] [CrossRef]
- Yang, X.; Xu, H.; Shao, L.; Li, T.; Wang, Y.; Wang, R. Response of Photosynthetic Capacity of Tomato Leaves to Different LED Light Wavelength. Environ. Exp. Bot. 2018, 150, 161–171. [Google Scholar] [CrossRef]
- Matysiak, B.; Ropelewska, E.; Wrzodak, A.; Kowalski, A.; Kaniszewski, S. Yield and Quality of Romaine Lettuce at Different Daily Light Integral in an Indoor Controlled Environment. Agronomy 2022, 12, 1026. [Google Scholar] [CrossRef]
- Huerta, R.R.; Saldaña, M.D.A. Pressurized Fluid Treatment of Barley and Canola Straws to Obtain Carbohydrates and Phenolics. J. Supercrit. Fluids 2018, 141, 12–20. [Google Scholar] [CrossRef]
- Fan, K.; Li, F.; Chen, X.; Li, Z.; Mulla, D.J. Nitrogen Balance Index Prediction of Winter Wheat by Canopy Hyperspectral Transformation and Machine Learning. Remote Sens. 2022, 14, 3504. [Google Scholar] [CrossRef]
- da Silva Junior, C.A.; Nanni, M.R.; Shakir, M.; Teodoro, P.E.; de Oliveira-Júnior, J.F.; Cezar, E.; de Gois, G.; Lima, M.; Wojciechowski, J.C.; Shiratsuchi, L.S. Soybean Varieties Discrimination Using Non-Imaging Hyperspectral Sensor. Infrared Phys. Technol. 2018, 89, 338–350. [Google Scholar] [CrossRef]
- Guo, T.; Tan, C.; Li, Q.; Cui, G.; Li, H. Estimating Leaf Chlorophyll Content in Tobacco Based on Various Canopy Hyperspectral Parameters. J. Ambient Intell. Humaniz. Comput. 2019, 10, 3239–3247. [Google Scholar] [CrossRef]
- Zhang, H.; Zhang, L.; Wang, S.; Zhang, L. Online Water Quality Monitoring Based on UV–Vis Spectrometry and Artificial Neural Networks in a River Confluence near Sherfield-on-Loddon. Environ. Monit. Assess. 2022, 194, 630. [Google Scholar] [CrossRef]
- Zhou, Q.; Yu, L.; Zhang, X.; Liu, Y.; Zhan, Z.; Ren, L.; Luo, Y. Fusion of UAV Hyperspectral Imaging and LiDAR for the Early Detection of EAB Stress in Ash and a New EAB Detection Index NDVI(776,678). Remote Sens. 2022, 14, 2428. [Google Scholar] [CrossRef]
- Giordano, M.; El-Nakhel, C.; Carillo, P.; Colla, G.; Graziani, G.; Di Mola, I.; Mori, M.; Kyriacou, M.C.; Rouphael, Y.; Soteriou, G.A.; et al. Plant-Derived Biostimulants Differentially Modulate Primary and Secondary Metabolites and Improve the Yield Potential of Red and Green Lettuce Cultivars. Agronomy 2022, 12, 1361. [Google Scholar] [CrossRef]
- Shi, M.; Gu, J.; Wu, H.; Rauf, A.; Bin Emran, T.; Khan, Z.; Mitra, S.; Aljohani, A.S.M.; Alhumaydhi, F.A.; Al-Awthan, Y.S.; et al. Phytochemicals, Nutrition, Metabolism, Bioavailability, and Health Benefits in Lettuce: A Comprehensive Review. Antioxidants 2022, 11, 1158. [Google Scholar] [CrossRef]
- Wang, L.; Chang, Q.; Li, F.; Yan, L.; Huang, Y.; Wang, Q.; Luo, L. Effects of Growth Stage Development on Paddy Rice Leaf Area Index Prediction Models. Remote Sens. 2019, 11, 361. [Google Scholar] [CrossRef] [Green Version]
- Jin, J.; Wang, Q. Selection of Informative Spectral Bands for PLS Models to Estimate Foliar Chlorophyll Content Using Hyperspectral Reflectance. IEEE Trans. Geosci. Remote Sens. 2019, 57, 3064–3072. [Google Scholar] [CrossRef]
- Guardado Yordi, E.; Koelig, R.; Matos, M.J.; Pérez Martínez, A.; Caballero, Y.; Santana, L.; Pérez Quintana, M.; Molina, E.; Uriarte, E. Artificial Intelligence Applied to Flavonoid Data in Food Matrices. Foods 2019, 8, 573. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Cezar, E.; Nanni, M.R.; Guerrero, C.; da Silva Junior, C.A.; Cruciol, L.G.T.; Chicati, M.L.; Silva, G.F.C. Organic Matter and Sand Estimates by Spectroradiometry: Strategies for the Development of Models with Applicability at a Local Scale. Geoderma 2019, 340, 224–233. [Google Scholar] [CrossRef]
- Koh, J.C.O.; Banerjee, B.P.; Spangenberg, G.; Kant, S. Automated Hyperspectral Vegetation Index Derivation Using a Hyperparameter Optimisation Framework for High-Throughput Plant Phenotyping. New Phytol. 2022, 233, 2659–2670. [Google Scholar] [CrossRef]
- Rodrigues, M.; Berti de Oliveira, R.; Leboso Alemparte Abrantes dos Santos, G.; Mayara de Oliveira, K.; Silveira Reis, A.; Herrig Furlanetto, R.; Antônio Yanes Bernardo Júnior, L.; Silva Coelho, F.; Rafael Nanni, M. Rapid Quantification of Alkaloids, Sugar and Yield of Tobacco (Nicotiana tabacum L.) Varieties by Using Vis–NIR–SWIR Spectroradiometry. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 2022, 274, 121082. [Google Scholar] [CrossRef]
- Kior, A.; Sukhov, V.; Sukhova, E. Application of Reflectance Indices for Remote Sensing of Plants and Revealing Actions of Stressors. Photonics 2021, 8, 582. [Google Scholar] [CrossRef]
- Ferri, C.P.; Formaggio, A.R.; Schiavinato, M.A. Narrow Band Spectral Indexes for Chlorophyll Determination in Soybean Canopies [Glycine max (L.) Merril]. Braz. J. Plant Physiol. 2004, 16, 131–136. [Google Scholar] [CrossRef]
- Jin, J.; Huang, N.; Huang, Y.; Yan, Y.; Zhao, X.; Wu, M. Proximal Remote Sensing-Based Vegetation Indices for Monitoring Mango Tree Stem Sap Flux Density. Remote Sens. 2022, 14, 1483. [Google Scholar] [CrossRef]
- Ryu, J.H.; Jeong, H.; Cho, J. Performances of Vegetation Indices on Paddy Rice at Elevated Air Temperature, Heat Stress, and Herbicide Damage. Remote Sens. 2020, 12, 2654. [Google Scholar] [CrossRef]
- Crusiol, L.G.T.; Sun, L.; Sibaldelli, R.N.R.; Junior, V.F.; Furlaneti, W.X.; Chen, R.; Sun, Z.; Wuyun, D.; Chen, Z.; Nanni, M.R.; et al. Strategies for Monitoring Within-Field Soybean Yield Using Sentinel-2 Vis-NIR-SWIR Spectral Bands and Machine Learning Regression Methods. Precis. Agric. 2022, 23, 1093–1123. [Google Scholar] [CrossRef]
- Gitelson, A.; Merzlyak, M.N. Spectral Reflectance Changes Associated with Autumn Senescence of Aesculus hippocastanum L. and Acer platanoides L. Leaves. Spectral Features and Relation to Chlorophyll Estimation. J. Plant Physiol. 1994, 143, 286–292. [Google Scholar] [CrossRef]
- Stimson, H.C.; Breshears, D.D.; Ustin, S.L.; Kefauver, S.C. Spectral Sensing of Foliar Water Conditions in Two Co-Occurring Conifer Species: Pinus edulis and Juniperus monosperma. Remote Sens. Environ. 2005, 96, 108–118. [Google Scholar] [CrossRef]
- Lichtenthaler, H.K. Vegetation Stress: An Introduction to the Stress Concept in Plants. J. Plant Physiol. 1996, 148, 4–14. [Google Scholar] [CrossRef]
- Chappelle, E.W.; Kim, M.S.; McMurtrey, J.E. Ratio Analysis of Reflectance Spectra (RARS): An Algorithm for the Remote Estimation of the Concentrations of Chlorophyll A, Chlorophyll B, and Carotenoids in Soybean Leaves. Remote Sens. Environ. 1992, 39, 239–247. [Google Scholar] [CrossRef]
- Gitelson, A.A.; Zur, Y.; Chivkunova, O.B.; Merzlyak, M.N. Assessing Carotenoid Content in Plant Leaves with Reflectance Spectroscopy. Photochem. Photobiol. 2002, 75, 272. [Google Scholar] [CrossRef]
- Blackburn, G.A. Spectral Indices for Estimating Photosynthetic Pigment Concentrations: A Test Using Senescent Tree Leaves. Int. J. Remote Sens. 1998, 19, 657–675. [Google Scholar] [CrossRef]
- Merzlyak, M.N.; Gitelson, A.A.; Chivkunova, O.B.; Rakitin, V.Y.U. Non-Destructive Optical Detection of Pigment Changes during Leaf Senescence and Fruit Ripening. Physiol. Plant. 1999, 106, 135–141. [Google Scholar] [CrossRef] [Green Version]
- Vogelmann, J.E.; Rock, B.N.; Moss, D.M. Red Edge Spectral Measurements from Sugar Maple Leaves. Int. J. Remote Sens. 1993, 14, 1563–1575. [Google Scholar] [CrossRef]
- Hunt, E.R.; Rock, B.N. Detection of Changes in Leaf Water Content Using Near- and Middle-Infrared Reflectances. Remote Sens. Environ. 1989, 30, 43–54. [Google Scholar]
- Peñuelas, J.; Filella, I.; Gamon, J.A. Assessment of Photosynthetic Radiation-Use Efficiency with Spectral Reflectance. New Phytol. 1995, 131, 291–296. [Google Scholar] [CrossRef]
- Metternicht, G. Vegetation Indices Derived from High-Resolution Airborne Videography for Precision Crop Management. Int. J. Remote Sens. 2003, 24, 2855–2877. [Google Scholar] [CrossRef]
PLSR Models | Attributes | PLSR Parameters | |||||
---|---|---|---|---|---|---|---|
r | R2 | Offset | RMSE | RPD | Bias | ||
Predicted | Chla (mg m−2) | 0.92 | 0.85 | 55.6 | 55.5 | 2.6 | 0.001 |
Chlb (mg m−2) | 0.92 | 0.84 | 57.1 | 57.1 | 2.5 | 0.001 | |
Chla+b (mg m−2) | 0.93 | 0.86 | 108.1 | 106.1 | 2.7 | 0.001 | |
Car (mg m−2) | 0.94 | 0.89 | 16.9 | 17.0 | 3.0 | 0.001 | |
AnC (nmol m−2) | 0.89 | 0.79 | 0.3 | 0.3 | 2.2 | 0.001 | |
Flv (nmol m−2) | 0.92 | 0.85 | 10.9 | 10.8 | 2.5 | 1.59 | |
Chla (mg g−1) | 0.94 | 0.88 | 2.6 | 2.6 | 2.8 | 0.001 | |
Chlb (mg g−1) | 0.89 | 0.79 | 1.5 | 1.5 | 2.2 | 0.001 | |
Chla+b (mg g−1) | 0.93 | 0.87 | 3.7 | 3.7 | 2.8 | 0.2 | |
Car (mg g−1) | 0.93 | 0.86 | 0.8 | 0.8 | 2.7 | 0.01 | |
AnC (µmol g−1) | 0.88 | 0.77 | 0.1 | 0.1 | 2.1 | 0.02 | |
Flv (µmol g−1) | 0.93 | 0.87 | 2.2 | 2.2 | 2.8 | 0.32 |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Falcioni, R.; Antunes, W.C.; Demattê, J.A.M.; Nanni, M.R. Reflectance Spectroscopy for the Classification and Prediction of Pigments in Agronomic Crops. Plants 2023, 12, 2347. https://0-doi-org.brum.beds.ac.uk/10.3390/plants12122347
Falcioni R, Antunes WC, Demattê JAM, Nanni MR. Reflectance Spectroscopy for the Classification and Prediction of Pigments in Agronomic Crops. Plants. 2023; 12(12):2347. https://0-doi-org.brum.beds.ac.uk/10.3390/plants12122347
Chicago/Turabian StyleFalcioni, Renan, Werner Camargos Antunes, José Alexandre M. Demattê, and Marcos Rafael Nanni. 2023. "Reflectance Spectroscopy for the Classification and Prediction of Pigments in Agronomic Crops" Plants 12, no. 12: 2347. https://0-doi-org.brum.beds.ac.uk/10.3390/plants12122347