Anti-Cancer Properties of Flaxseed Proteome

Round 1

Reviewer 1 Report

The argument you make about the potential mechanism of flaxseed proteins in inhibiting cancer cell proliferation and metastasis is good. However, it needs to be revised and supplemented with the following

1)        Provide examples of laboratory or clinical studies that support this idea, and how the experiments were designed to obtain the results.

2)        Discuss the potential application of flaxseed proteins in cancer therapy, e.g., through the design of formulations to realize their anticancer effects.

3)        You suggest that more research is needed, and you can envision the focus of future research, such as in-depth study of certain key proteins or signaling pathways.

4)        You can summarize the limitations and shortcomings of the current research, and put forward your own suggestions or high-level thinking.

5)        The overall structure of the paper is clear, the arguments are clear, and the content is systematically organized, but examples and data can be added to support the strength of the arguments.

6)        Please add some descriptions of the chemical components and give the corresponding chemical structures

7)        Please give a schematic diagram of the mechanism to make it easier for the reader to understand.

The argument you make about the potential mechanism of flaxseed proteins in inhibiting cancer cell proliferation and metastasis is good. However, it needs to be revised and supplemented with the following

1)        Provide examples of laboratory or clinical studies that support this idea, and how the experiments were designed to obtain the results.

2)        Discuss the potential application of flaxseed proteins in cancer therapy, e.g., through the design of formulations to realize their anticancer effects.

3)        You suggest that more research is needed, and you can envision the focus of future research, such as in-depth study of certain key proteins or signaling pathways.

4)        You can summarize the limitations and shortcomings of the current research, and put forward your own suggestions or high-level thinking.

5)        The overall structure of the paper is clear, the arguments are clear, and the content is systematically organized, but examples and data can be added to support the strength of the arguments.

6)        Please add some descriptions of the chemical components and give the corresponding chemical structures

7)        Please give a schematic diagram of the mechanism to make it easier for the reader to understand.

Author Response

Dear Reviewer,

Thank you for your valuable feedback. We appreciate your acknowledgment of the potential mechanism of flaxseed proteins in inhibiting cancer cell proliferation and metastasis.

In response to your suggestion for revision and supplementation, we are committed to enhancing the clarity and completeness of our argument. Please find our answers in the attached file.

We look forward to your continued guidance and appreciate the opportunity to improve our work.

Best regards,

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments for author File: Comments.pdf

Author Response

Dear Reviewer,

Thank you for your thoughtful evaluation of our manuscript, "Anti-cancer properties of flaxseed proteome." We appreciate your positive feedback on the overall content, acknowledging the focus on the anti-cancer properties of flaxseed proteins and their potential application in cancer treatment.

We are grateful for your suggestion that the manuscript could be suitable for publication in Proteomes after some modifications and additions. Your comments are invaluable, and we are eager to address them to enhance the manuscript further.

Please find our responses and revisions to your comments detailed in the attached file.

We look forward to your continued guidance in refining our work for potential publication.

 Best regards,

Author Response File: Author Response.pdf

Reviewer 3 Report

The review article on the anti-cancer potential of flax proteins is written correctly. It summarizes the latest knowledge about the properties of individual proteins and amino acids isolated from flax. The article has the potential for publication.

Line 207: H2SO4, please use subscripts. 

Author Response

Dear Reviewer,

Thank you for your positive feedback on our review article exploring the anti-cancer potential of flax proteins. We are delighted to hear that you find the article well-written and that it effectively summarizes the latest knowledge about the properties of individual proteins and amino acids isolated from flax.

We appreciate your acknowledgment of the article's potential for publication. Your encouraging words motivate us to continue our efforts in contributing valuable insights to the field.

In response to your suggestion, we have made the necessary adjustments to the chemical notation. The sulfuric acid is now correctly represented as H₂SO₄ with subscripts, ensuring accurate and clear depiction in our manuscript.

Thank you once again for your time and thoughtful evaluation.

Best regards

Reviewer 4 Report

This review article is of some interest but, in my opinion it cannot be published in the current form. Below are summarized my concerns:

- The paragraphs dedicated to proteoform analysis(lines 188-194) and extraction of aminoacids (lines 204-212) have been treated too concisely. While understanding that they do not represent an essential part of the article, I think that a few additional lines should briefly describe which of the MS-based methods have been used to identify the PTMs. The same goes for the amino acid determination. The use of HCl or H₂SO₄ poses problems with specific aminoacids that should be at least mentioned. How and when the enzimatic digestion can be used ?

-Tables 2 and 3 are not acceptable in their current form. The written part should be abbreviated keeping only essential details and the bibliography entry.

-Table 4 is redundant. The information under the column "Description" is the same for all aminoacids. The structure of the aminoacids is well-known. The only information of interest in this table is that of the last column (composition from flax seed proteins). The table should be reconsidered (may be deleted ??) in the light of this criticism. 

The language is good, only minor editing being necessary.

Author Response

Dear Reviewer,

Thank you for your thorough evaluation of our review article on the anti-cancer potential of flax proteins. We appreciate the time and effort you have invested in providing constructive feedback. Your insights are invaluable to us as we strive to enhance the quality of our work.

In response to your concerns, we have carefully reviewed and addressed each point raised. Please find in the attached file our revisions and clarifications to better align the manuscript with the standards for publication.

We sincerely appreciate your guidance in refining our article and look forward to your continued feedback.

 Best regards,

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Can be accepted in current form

Reviewer 4 Report

The authors have significantly modified the text and, in my opinion, the quality of the review has improved. I am satisfied of the changes introduced and my suggestion is that this article is now ready for publication in proteomes.