Circulating Total Extracellular Vesicles Cargo Are Associated with Age-Related Oxidative Stress and Susceptibility to Cardiovascular Diseases: Exploratory Results from Microarray Data
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Circulating Extracellular Vesicle and Particle Isolation and Characterization
2.3. microRNA Analysis
2.4. Western Blot for Pro- and Antioxidant Machinery
2.5. Determination of Pro- and Antioxidant Enzyme Activities
2.6. Statistical Analysis
3. Results
3.1. Circulating Total EV microRNA Expression Is Altered by Aging
3.2. Cardiovascular Signaling and Redox Pathways Are Predicted to Be Impacted by Circulating Total EV microRNA from Aged Animals
3.3. Redox Machinery in EVPs
3.4. Characterization of Isolated EVPs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cechinel, L.R.; Batabyal, R.A.; Blume Corssac, G.; Goldberg, M.; Harmon, B.; Vallejos, V.M.R.; Bruch, G.E.; Massensini, A.R.; Belló-Klein, A.; Araujo, A.S.d.R.; et al. Circulating Total Extracellular Vesicles Cargo Are Associated with Age-Related Oxidative Stress and Susceptibility to Cardiovascular Diseases: Exploratory Results from Microarray Data. Biomedicines 2023, 11, 2920. https://0-doi-org.brum.beds.ac.uk/10.3390/biomedicines11112920
Cechinel LR, Batabyal RA, Blume Corssac G, Goldberg M, Harmon B, Vallejos VMR, Bruch GE, Massensini AR, Belló-Klein A, Araujo ASdR, et al. Circulating Total Extracellular Vesicles Cargo Are Associated with Age-Related Oxidative Stress and Susceptibility to Cardiovascular Diseases: Exploratory Results from Microarray Data. Biomedicines. 2023; 11(11):2920. https://0-doi-org.brum.beds.ac.uk/10.3390/biomedicines11112920
Chicago/Turabian StyleCechinel, Laura Reck, Rachael Ann Batabyal, Giana Blume Corssac, Madeleine Goldberg, Brennan Harmon, Virgínia Mendes Russo Vallejos, Gisele E. Bruch, André Ricardo Massensini, Adriane Belló-Klein, Alex Sander da Rosa Araujo, and et al. 2023. "Circulating Total Extracellular Vesicles Cargo Are Associated with Age-Related Oxidative Stress and Susceptibility to Cardiovascular Diseases: Exploratory Results from Microarray Data" Biomedicines 11, no. 11: 2920. https://0-doi-org.brum.beds.ac.uk/10.3390/biomedicines11112920