Skin-Beautifying Effects of Magnolol and Honokiol Glycosides

Round 1

Reviewer 1 Report

The authors present a study about the anti-ageing effects of Magnolol and Honokiol glycosides.

The article is interesting and well organized, and the results are promising. And potentially useful for the cosmetic industry.

Lines 49-54: Please add a reference.

Lines 144-150: Please check the sentences. It is confusing, and some sentences seem incomplete or out of place. Example: "After being stirred for 30 minutes at room temperature." What happened after being stirred for 30 min? Was the solution filtered through or with the Dowex resins?

Lines 190 to 202: Please rewrite this section, considering: do not use personal pronouns such as we in the description. It seems that some sentences are incomplete, and it is not understood the meaning of "a condition of 15 000 rpm in 4 min." 

Lines 368-370: This sentence is not sufficiently discussed. Are there substantial differences between the two glycosides?  

Author Response

To Reviewers

              Thank you very much for valuable suggestions and comments to our manuscript.   According to the suggestions of the reviewer, we prepared a revised manuscript.   Followings are major points of revision and our comment.

              We agree with the suggestion of reviewer.   Therefore, We changed everything according to the suggestion of reviewer.

Lines 49-54: Please add a reference.

---> We added references, according to suggestion.

Lines 144-150: Please check the sentences. It is confusing, and some sentences seem incomplete or out of place. Example: "After being stirred for 30 minutes at room temperature." What happened after being stirred for 30 min? Was the solution filtered through or with the Dowex resins?

---> We corrected it like a Lines 145-148.

Lines 190 to 202: Please rewrite this section, considering: do not use personal pronouns such as we in the description. It seems that some sentences are incomplete, and it is not understood the meaning of "a condition of 15 000 rpm in 4 min."

---> We corrected it like a Lines 194-206.

Lines 368-370: This sentence is not sufficiently discussed. Are there substantial differences between the two glycosides? 

---> We added “four glycosides” like a Line 376. No differences between the two glycosides were found, but both glycosides have shown remarkable anti-glycation activity.

Author Response File: Author Response.docx

Reviewer 2 Report

Provide the basic physicochemical (color, sunstance, m.p. etc...)  data before making so many claims in the conclusion.

Author Response

To Reviewers

              Thank you very much for valuable suggestions and comments to our manuscript.   According to the suggestions of the reviewer, we prepared a revised manuscript.   Followings are major points of revision and our comment.

              We agree with the suggestion of reviewer.   Therefore, We changed everything according to the suggestion of reviewer.

Provide the basic physicochemical (color, sunstance, m.p. etc...)  data before making so many claims in the conclusion.

---> We added “White crystal, m.p.” like Lines 154, 164, 173 and 183.

Author Response File: Author Response.docx

Reviewer 3 Report

The work presents very interesting results. I recommend its acceptance after a few minor changes to improve the quality of the manuscript.

Include the objective of the work explicitly at the end of the introduction. The objective of this work was…

Change hours to h throughout the script

Cell viability (%) = (absorbance of Sample − DMEM/absorbance of control) × 100 could be: Cell viability (%) = (absorbance of Sample – absorbance DMEM/absorbance of control) × 100

Cell viability (%) = (absorbance of GO-DMEM / absorbance of control) × 100 could be: Cell viability (%) = (absorbance of GO- absorbance DMEM / absorbance of control) × 100

Cell viability (%) = (absorbance of GO-DMEM, absorbance of sample-GO-DMEM /absorbance of control) × 100 could be: Cell viability (%) = (absorbance of GO- absorbance DMEM, absorbance of sample-GO- absorbance DMEM /absorbance of control) × 100

In the statistical analysis, a one way ANOVA analysis was not carried out?

Figure 2. Include cell line and statistical analysis, include symbols for statistical differences in bars

In figure 3 move the labels to another place and put concentration on the x axis

Numerical data have been included in the conclusions "have shown significant anti-glycation activities of less than 0.10 mM in IC50". They are already in the abstract and in the results. They should not be repeated again. In general, the conclusions are similar to the abstract, so they must be presented in another way.

Author Response

To Reviewers

              Thank you very much for valuable suggestions and comments to our manuscript.   According to the suggestions of the reviewer, we prepared a revised manuscript.   Followings are major points of revision and our comment.

              We agree with the suggestion of reviewer.   Therefore, we changed everything according to the suggestion of reviewer.

Change hours to h throughout the script

---> We corrected hours to h.

Cell viability (%) = (absorbance of Sample − DMEM/absorbance of control) × 100 could be: Cell viability (%) = (absorbance of Sample – absorbance DMEM/absorbance of control) × 100

---> We corrected it, according to suggestion.

Cell viability (%) = (absorbance of GO-DMEM / absorbance of control) × 100 could be: Cell viability (%) = (absorbance of GO- absorbance DMEM / absorbance of control) × 100

---> We corrected it, according to suggestion.

Cell viability (%) = (absorbance of GO-DMEM, absorbance of sample-GO-DMEM /absorbance of control) × 100 could be: Cell viability (%) = (absorbance of GO- absorbance DMEM, absorbance of sample-GO- absorbance DMEM /absorbance of control) × 100

---> We corrected it, according to suggestion.

In the statistical analysis, a one way ANOVA analysis was not carried out?

---> We corrected + to “±”.

Figure 2. Include cell line and statistical analysis, include symbols for statistical differences in bars

---> We added symbols for statistical differences in bars.

In figure 3 move the labels to another place and put concentration on the x axis

---> We added “concentration (mM)”.

Numerical data have been included in the conclusions "have shown significant anti-glycation activities of less than 0.10 mM in IC50". They are already in the abstract and in the results. They should not be repeated again. In general, the conclusions are similar to the abstract, so they must be presented in another way.

---> We corrected the conclusions.

Author Response File: Author Response.docx