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Article

Sirtuin 3 Restores Synthesis and Secretion of Very Low-Density Lipoproteins in Cow Hepatocytes Challenged with Nonesterified Fatty Acids In Vitro

1
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
2
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this publication.
Academic Editor: Antonio Humberto Hamad Minervino
Received: 26 April 2021 / Revised: 2 June 2021 / Accepted: 9 June 2021 / Published: 30 June 2021
(This article belongs to the Special Issue Animal Nutritional and Metabolic Diseases)
Fatty liver is closely associated with elevated concentrations of nonesterified fatty acids (NEFA) and a low level of very low-density lipoproteins (VLDL) in blood of dairy cows. High NEFA inhibit the VLDL synthesis and assembly, and cause hepatic triacylglycerol (TAG) deposition. Sirtuin 3 (SIRT3), a mitochondrial deacetylase, antagonizes NEFA-induced TAG accumulation through modulating expressions of fatty acid synthesis and oxidation genes in cow hepatocytes. However, the role of SIRT3 in the VLDL synthesis and assembly was largely unknown. Here we aimed to test whether SIRT3 would recover the synthesis and assembly of VLDL in cow hepatocytes induced by high NEFA. Primary cow hepatocytes were isolated from 3 Holstein cows. Hepatocytes were infected with SIRT3 overexpression adenovirus (Ad-SIRT3), SIRT3-short interfering (si) RNA, or first infected with Ad-SIRT3 and then incubated with 1.0 mM NEFA (Ad-SIRT3 + NEFA). Expressions of key genes in VLDL synthesis and the VLDL contents in cell culture supernatants were measured. SIRT3 overexpression significantly increased the mRNA abundance of microsomal triglyceride transfer protein (MTP), apolipoprotein B100 (ApoB100) and ApoE (p < 0.01), and raised VLDL contents in the supernatants (p < 0.01). However, SIRT3 silencing displayed a reverse effect in comparison to SIRT3 overexpression. Compared with NEFA treatment alone, the Ad-SIRT3 + NEFA significantly upregulated the mRNA abundance of MTP, ApoB100 and ApoE (p < 0.01), and increased VLDL contents in the supernatants (p < 0.01). Our data demonstrated that SIRT3 restored the synthesis and assembly of VLDL in cow hepatocytes challenged with NEFA, providing an in vitro basis for further investigations testing its feasibility against hepatic TAG accumulation in dairy cows during the perinatal period. View Full-Text
Keywords: animal metabolic diseases; fat metabolism; MTP; ApoB100; ApoE; perinatal period animal metabolic diseases; fat metabolism; MTP; ApoB100; ApoE; perinatal period
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MDPI and ACS Style

Xing, D.; Wang, B.; Lu, H.; Peng, T.; Su, J.; Lei, H.; He, J.; Zhou, Y.; Liu, L. Sirtuin 3 Restores Synthesis and Secretion of Very Low-Density Lipoproteins in Cow Hepatocytes Challenged with Nonesterified Fatty Acids In Vitro. Vet. Sci. 2021, 8, 121. https://0-doi-org.brum.beds.ac.uk/10.3390/vetsci8070121

AMA Style

Xing D, Wang B, Lu H, Peng T, Su J, Lei H, He J, Zhou Y, Liu L. Sirtuin 3 Restores Synthesis and Secretion of Very Low-Density Lipoproteins in Cow Hepatocytes Challenged with Nonesterified Fatty Acids In Vitro. Veterinary Sciences. 2021; 8(7):121. https://0-doi-org.brum.beds.ac.uk/10.3390/vetsci8070121

Chicago/Turabian Style

Xing, Dongmei, Baogen Wang, Hong Lu, Tao Peng, Jianming Su, Hongyu Lei, Jianhua He, Yingfang Zhou, and Lei Liu. 2021. "Sirtuin 3 Restores Synthesis and Secretion of Very Low-Density Lipoproteins in Cow Hepatocytes Challenged with Nonesterified Fatty Acids In Vitro" Veterinary Sciences 8, no. 7: 121. https://0-doi-org.brum.beds.ac.uk/10.3390/vetsci8070121

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