Continuous Bioproduction of Alginate Bacterial under Nitrogen Fixation and Nonfixation Conditions

Round 1

Reviewer 1 Report

Accepted

Thank You very much for the opportunity to review this manuscript.

Author Response

The reviewer does not include specific observations.

Reviewer 2 Report

I am very grateful you for the invitation to review manuscript fermentation-2366721 by Contreras-Abara and coauthors "Continuous bioproduction of alginate bacteria under nitrogen fixation and nonfixation conditions”. The aim of this study was to develop a continuous bioprocess and a comparative analysis of alginate production under diazotrophic and nondiazotrophic conditions. The work is interesting but needs adjustments to increase the quality of the material.

 

Comments:

- Abstract: Insert the alginate bioproduction mechanism.

- Lines 11-12: Applications and characteristics are different information.

- Abstract: Please indicate in the abstract a brief and better step-by-step about the work and the evaluated parameters.

- Line 23: Change the repeated keywords by different words from the title.

 - Line 28: Insert other applications to justify the term “diverse”.

- Lines 41-43: Algae production can be considered a bioprocess.

- Line 58: check the correct citation of references.

- Introduction: Review the presentation of papers in the introduction. The details should be presented in the discussion. The introduction should be used to present general aspects.

- Introduction: Include a better explanation of diazotrophic and nondiazotrophic conditions.

- Lines 109, 123 and others: Please standardize the units (minutes, min, etc).

- Line 180: Remove the term “Figure 1” from the figure.

- Line 267: Remove the term “Figure 3” from the figure.

- Results: Include an improved discussion of yield and process versus conventional alginate sources.

- Results and discussion: The authors should improve the discussion of the advantage of using alginate production by the suggested process in relation to the conventional process.

- General comments: The demand for alginate is unclear. Include market information, quantity used in the most diverse processes, among other information.

- Discuss in relation to the cost of the process in relation to the conventional procurement process. Also, specify the conventional process for better comparison.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 3 Report

Good experimental plan and methods applied. Discussions of the obtained results should be improved and conclusion reviewed.

I suggest minor revisions before publication.

Comments for author File: Comments.pdf

Minor editing of English form is required.

Author Response

Please see the attachment

Author Response File: Author Response.pdf