Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Influence of PGRs on S. rebaudiana Morphogenesis In Vitro
2.3. Influence of Light Factors on the Morphophysiological Parameters of S. rebaudiana In Vitro
2.4. The Combined Effect of Physical and PGRs Factors on the Morphophysiological Parameters of In Vitro S. rebaudiana Microshoot Growth
2.5. Phenotypic Analysis of Plants
2.6. Quantitative Determination of Photosynthetic Pigments of S. rebaudiana In Vivo
2.7. Qualitative and Semi-Quantitative Determination of Diterpene Glycosides in Dry Leaves of S. rebaudiana In Vivo
2.8. Quantitative Determination of Steviosides by High-Performance Liquid Chromatography (HPLC) of Dry Leaves of S. rebaudiana In Vivo
2.9. Statistical Analysis of Experimental Data
3. Results and Discussion
3.1. Influence of PGRs on In Vitro S. rebaudiana Clonal Propagation and Microshoot Morphology
3.2. Influence of Physical Factors on the Morphophysiological Parameters of S. rebaudiana In Vitro
3.2.1. Effect of Red-Blue LED Lighting on Morphophysiological Parameters of S. rebaudiana Microshoots
3.2.2. The Effect of Coherent Light Treatment on the Morphophysiological Characteristics of S. rebaudiana Microshoots In Vitro
3.2.3. The Effect of Monochrome Light Treatment on the Morphophysiological Characteristics of S. rebaudiana Microshoots In Vitro
3.2.4. The Combined Effect of Physical and PGRs Factors on the Morphogenesis of S. rebaudiana Microshoots Culture In Vitro
Monochrome Light Treatment in Combination with PGRs under In Vitro Conditions
Coherent Light Treatment in Combination with PGRs under In Vitro Conditions
3.3. Effects of Red-Blue LED Lighting on the Biochemical Characteristics of S. rebaudiana Plants In Vivo
3.3.1. Quantitative Determination of Photosynthetic Pigments
3.3.2. Qualitative and Semi-Quantitative Determination of Diterpene Glycosides by TLC
3.3.3. Quantitative Determination of Stevioside by HPLC
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shulgina, A.A.; Kalashnikova, E.A.; Tarakanov, I.G.; Kirakosyan, R.N.; Cherednichenko, M.Y.; Polivanova, O.B.; Baranova, E.N.; Khaliluev, M.R. Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo. Horticulturae 2021, 7, 195. https://0-doi-org.brum.beds.ac.uk/10.3390/horticulturae7070195
Shulgina AA, Kalashnikova EA, Tarakanov IG, Kirakosyan RN, Cherednichenko MY, Polivanova OB, Baranova EN, Khaliluev MR. Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo. Horticulturae. 2021; 7(7):195. https://0-doi-org.brum.beds.ac.uk/10.3390/horticulturae7070195
Chicago/Turabian StyleShulgina, Alla A., Elena A. Kalashnikova, Ivan G. Tarakanov, Rima N. Kirakosyan, Mikhail Yu. Cherednichenko, Oksana B. Polivanova, Ekaterina N. Baranova, and Marat R. Khaliluev. 2021. "Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo" Horticulturae 7, no. 7: 195. https://0-doi-org.brum.beds.ac.uk/10.3390/horticulturae7070195