Dictyostelium Cell Fixation: Two Simple Tricks
Abstract
:1. Introduction
2. Results
2.1. Cell Flattening
2.2. Cell Attachment
3. Discussion
4. Experimental Design
4.1. Centrifugation
4.2. D. discoideum Fixative
- 50% PHEM buffer (30 mM PIPES, 12.5 mM HEPES, pH 6.9, 4 mM EGTA, 1 mM MgCl2). pH 6.9
- 3.7% formaldehyde
- 0.05% glutaraldehyde
- 0.1% Triton X-100
4.3. Phosphate Development Buffer
- 20 mM KCL
- 2.5 mM MgCl2·6H20
- 27 mM NaH2PO4·H20
- 24 mM Na2HPO4
- pH 6.4 (see reference [12] for detail)
4.4. Light Microscopy
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Koonce, M.; Tikhonenko, I.; Gräf, R. Dictyostelium Cell Fixation: Two Simple Tricks. Methods Protoc. 2020, 3, 47. https://0-doi-org.brum.beds.ac.uk/10.3390/mps3030047
Koonce M, Tikhonenko I, Gräf R. Dictyostelium Cell Fixation: Two Simple Tricks. Methods and Protocols. 2020; 3(3):47. https://0-doi-org.brum.beds.ac.uk/10.3390/mps3030047
Chicago/Turabian StyleKoonce, Michael, Irina Tikhonenko, and Ralph Gräf. 2020. "Dictyostelium Cell Fixation: Two Simple Tricks" Methods and Protocols 3, no. 3: 47. https://0-doi-org.brum.beds.ac.uk/10.3390/mps3030047