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Acknowledgment to Reviewers of Surgeries in 2020
Article

Extracellular Vesicles in Autologous Cell Salvaged Blood in Orthopedic Surgery

1
Campus Prater, Sigmund Freud University, 1020 Vienna, Austria
2
Research Lab, Department of Surgery, Medical University of Vienna, 1090 Vienna, Austria
3
Austria Evangelical Hospital Vienna, 1180 Vienna, Austria
4
Core Facility Flow Cytometry, Medical University of Vienna, 1090 Vienna, Austria
*
Author to whom correspondence should be addressed.
Academic Editor: Nicola Maffulli
Received: 4 January 2021 / Revised: 1 February 2021 / Accepted: 4 February 2021 / Published: 8 February 2021
(1) Background: Cell salvage is highly recommended in orthopedic surgery to avoid allogeneic transfusions. Preparational steps during cell salvage may induce extracellular vesicle (EV) formation with potential thrombogenic activity. The purpose of our study was to assess the appearance of EVs at retransfusion. (2) Methods: After ethics committee approval and informed consent, blood was withdrawn from the autotransfusion system (Xtra, Sorin, Germany) of 23 patients undergoing joint arthroplasty. EVs were assessed by flow cytometry in two times centrifugated samples. EVs were stained with specific antibodies against cellular origins from platelets (CD41), myeloid cells (CD15), monocytes (CD14), and erythrocytes (CD235a). The measured events/µL in the flow cytometer were corrected to the number of EVs in the retransfusate. (3) Results: We measured low event rates of EVs from platelets and myeloid origin (<1 event/µL) and from monocytic origin (<2 events/µL). Mean event rates of 17,042 events/µL (range 12–81,164 events/µL) were found for EVs from red blood cells. (4) Conclusion: Retransfusate contains negligible amounts of potentially thrombogenic EVs from platelet and monocytic origin. Frequent EVs from erythrocytes may indicate red blood cell destruction and/or activation during autologous cell salvage. Further research is needed to investigate the clinical relevance of EVs from salvaged red blood cells. View Full-Text
Keywords: cell salvage; extracellular vesicle; microparticle cell salvage; extracellular vesicle; microparticle
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MDPI and ACS Style

Kutschera, M.; Pischlaeger, A.; Sztulman, L.; Kietaibl, S.; Spittler, A. Extracellular Vesicles in Autologous Cell Salvaged Blood in Orthopedic Surgery. Surgeries 2021, 2, 84-91. https://0-doi-org.brum.beds.ac.uk/10.3390/surgeries2010007

AMA Style

Kutschera M, Pischlaeger A, Sztulman L, Kietaibl S, Spittler A. Extracellular Vesicles in Autologous Cell Salvaged Blood in Orthopedic Surgery. Surgeries. 2021; 2(1):84-91. https://0-doi-org.brum.beds.ac.uk/10.3390/surgeries2010007

Chicago/Turabian Style

Kutschera, Maximilian, Agnes Pischlaeger, Larissa Sztulman, Sibylle Kietaibl, and Andreas Spittler. 2021. "Extracellular Vesicles in Autologous Cell Salvaged Blood in Orthopedic Surgery" Surgeries 2, no. 1: 84-91. https://0-doi-org.brum.beds.ac.uk/10.3390/surgeries2010007

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