Journal of Functional Morphology and Kinesiology

The extracellular matrix of the articular disc in a temporomandibular joint (TMJ) is composed mainly of collagen I and elastin. The collagen is important for resisting tensile forces, while the elastin is responsible to maintain the shape after deformation. We studied the orientation of collagen and elastin in a normal human temporomandibular joint disc by light microscopy, immunofluorescence and scanning electron microscopy. Our results demonstrated that collagen and elastin run parallel to each other in the intermediate zone with an anteroposterior orientation. From here, the orientation of two fibers groups changes into a disordered arrangement in the transition zone. Numerous elastic fibers cross with the collagen fibers, defining an interwoven knitted arrangement. The evaluation of the disc–condyle relationship shows that the medial margin of the articular disc is inserted directly at the superficial layer of the mandibular condylar cartilage. Therefore, the tensile properties of the TMJ disc are expressed in the directions corresponding to the orientation of the collagen fibers, and the complex orientation of elastin with the collagen determines the maintaining of the shape after the stresses by the joint movements. Moreover, the direct anatomical relationship between the articular disc and the mandibular condyle makes a decisive contribution to the understanding of TMJ movements. Full article

Background: Eccentric resistance training (ERT) in youth is advocated for aiding performance and injury risk. However, research investigating the applied practices of ERT in youth is in its infancy. In this study, we surveyed the perceptions and practices of practitioners utilizing ERT in youth to provide an understanding of its current application in practice. Methods: Sixty-four strength and conditioning coaches completed an online survey reporting their current use of ERT in youth using both open and closed questions. Results: Coaches deemed the inclusion of ERT important in youth with its inclusion based upon factors such as maturation status, training age and strength levels. Coaches also displayed an awareness of the physiological responses to eccentric exercise in youth compared to adults. ERT was primarily used for injury prevention, with the majority of coaches using body-weight and tempo exercises. Furthermore, utilizing eccentric hamstrings exercises was reported as highly important. The frequency of ERT tended to increase in older age groups and coaches mainly prescribed self-selected rest intervals. Finally, the need for further research into the training guidelines of ERT in youth was highlighted, in which coaches require more information on how maturation influences training adaptations and the fatigue–recovery responses. Conclusion: Coaches emphasized the importance of including ERT for both performance and injury prevention factors in youth although further research is required to generate practical guidelines for coaches in order to support its inclusion within practice. Full article

Ashwagandha (Withania somnifera) is considered a potent adaptogen and anti-stress agent that could have some potential to improve physical performance. This preferred reporting items for systematic reviews and meta-analyses (PRISMA)-based comprehensive systematic review and Bayesian meta-analysis aimed to evaluate clinical trials up to 2020 from PubMed, ScienceDirect, and Google Scholar databases regarding the effect of Ashwagandha supplementation on physical performance in healthy individuals. Besides implementing estimation statistics analysis, we developed Bayesian hierarchical models for a pre-specified subgroup meta-analysis on strength/power, cardiorespiratory fitness and fatigue/recovery variables. A total of 13 studies met the requirements of this systematic review, although only 12 were included in the quantitative analysis. A low-to-moderate overall risk of bias of the trials included in this study was detected. All Bayesian hierarchical models converged to a target distribution (Ȓ = 1) for both meta-analytic effect size (μ) and between-study standard deviation (τ). The meta-analytic approaches of the included studies revealed that Ashwagandha supplementation was more efficacious than placebo for improving variables related to physical performance in healthy men and female. In fact, the Bayesian models showed that future interventions might be at least in some way beneficial on the analyzed outcomes considering the 95% credible intervals for the meta-analytic effect size. Several practical applications and future directions are discussed, although more comparable studies are needed in exercise training, and athletic populations are needed to derive a more stable estimate of the true underlying effect. Full article

Participation in regular physical activity yields numerous psychological and physical health benefits. Despite this, a large proportion of the global population is increasingly becoming inactive and sedentary, which has been linked to various causes of morbidity and mortality. One practice that has been found to encourage healthy participation in physical activity and associated health behaviours is mindfulness. Mindfulness practices have been consistently linked to higher levels of physical activity participation. However, the relationship between mindfulness practices and physical activity remains ambiguous. This present paper comments on the role of mindfulness practice in physical activity and health behaviours. Implications for future research and practice have also been addressed. Full article

This study sought to investigate whether the stiffness of the biceps femoris long head differs between proximal and distal regions during isometric knee flexion at different contraction intensities and muscle lengths. Twelve healthy individuals performed knee flexion isometric contractions at 20% and 60% of maximum voluntary isometric contraction, with the knee flexed at 15 and 45 degrees. Muscle stiffness assessment was performed using ultrasound-based shear wave elastography. Proximal and distal regions of the biceps femoris long head were assessed. Biceps femoris long head muscle showed a greater stiffness (i) in the distal region, (ii) at higher contraction intensity, and (iii) at longer muscle length. The proximal-to-distal stiffness ratio was significantly lower than 1 (i.e., heterogenous) at lower contraction intensity regardless of the muscle length. However, this was not observed at higher contraction intensity. This study is the first to show heterogeneity in the active stiffness of the biceps femoris long head. Given the greater incidence of injury at the proximal region of biceps femoris long head, this study opens new directions for future research. Additionally, the present study results indicate that studies assessing muscle stiffness at one single muscle region should be interpreted with caution. Full article

The aim of this study was to compare male vs. female athletes in strength and power performance relative to body mass (BM) and lean body mass (LBM) and to investigate the relationships between muscle architecture and strength in both genders. Sixteen men (age = 26.4 ± 5.0 years; body mass = 88.9 ± 16.6 kg; height = 177.6 ± 9.3 cm) and fourteen women (age = 25.1 ± 3.2 years; body mass = 58.1 ± 9.1 kg; height = 161.7 ± 4.8 cm) were tested for body composition and muscle thickness (MT) of vastus lateralis muscle (VT), pectoralis major (PEC), and trapezius (TRAP). In addition, participants were tested for lower body power at countermovement jump (CMJP) and upper-body power at bench press throw (BPT). Participants were also assessed for one repetition maximum (1RM) at bench press (1RMBP), deadlift (1RMDE), and squat (1RMSQ). Significantly greater (p < 0.01) MT of the VL, PEC and TRAP muscles and LBM were detected in men compared to women. Significantly greater (p < 0.05) 1RMBP and BPT adjusted for LBM were detected in men than in women. No significant gender differences after adjusting for LBM were detected for 1RMSQ (p = 0.945); 1RMDE (p = 0.472) and CMJP (p = 0.656). Significantly greater (p < 0.05) results in all performance assessments adjusted for MT of the specific muscles, were detected in males compared to females. Superior performances adjusted for MT and LBM in men compared to women, may be related to gender differences in muscle morphology and LBM distribution, respectively. Full article

The purpose of this study was to investigate the effects of how limb dominance and joint immobilization alter markers of physical demand and muscle activation during ambulation with axillary crutches. In a crossover, counterbalanced study design, physically active females completed ambulation trials with three conditions: (1) bipedal walking (BW), (2) axillary crutch ambulation with their dominant limb (DOM), and (3) axillary crutch ambulation with their nondominant limb (NDOM). During the axillary crutch ambulation conditions, the non-weight-bearing knee joint was immobilized at a 30-degree flexion angle with a postoperative knee stabilizer. For each trial/condition, participants ambulated at 0.6, 0.8, and 1.0 mph for five minutes at each speed. Heart rate (HR) and rate of perceived exertion (RPE) were monitored throughout. Surface electromyography (sEMG) was used to record muscle activation of the medial gastrocnemius (MG), soleus (SOL), and tibialis anterior (TA) unilaterally on the weight-bearing limb. Biceps brachii (BB) and triceps brachii (TB) sEMG were measured bilaterally. sEMG signals for each immobilization condition were normalized to corresponding values for BW.HR (p < 0.001) and RPE (p < 0.001) were significantly higher for both the DOM and NDOM conditions compared to BW but no differences existed between the DOM and NDOM conditions (p > 0.05). No differences in lower limb muscle activation were noted for any muscles between the DOM and NDOM conditions (p > 0.05). Regardless of condition, BB activation ipsilateral to the ambulating limb was significantly lower during 0.6 mph (p = 0.005) and 0.8 mph (p = 0.016) compared to the same speeds for BB on the contralateral side. Contralateral TB activation was significantly higher during 0.6 mph compared to 0.8 mph (p = 0.009) and 1.0 mph (p = 0.029) irrespective of condition. In conclusion, limb dominance appears to not alter lower limb muscle activation and walking intensity while using axillary crutches. However, upper limb muscle activation was asymmetrical during axillary crutch use and largely dependent on speed. These results suggest that functional asymmetry may exist in upper limbs but not lower limbs during assistive device supported ambulation. Full article

Knee osteoarthritis (OA) is a chronic, degenerative, and progressive disease of articular cartilage, producing discomfort and physical disability in older adults. Thirteen percent of elderly people complain of knee OA. Management options for knee OA could be divided into the following categories: conservative, pharmacological, procedural, and surgical. Joint replacement is the gold standard, reserved for severe grades of knee OA, due to its complications rate and increased risk of joint revision. A nonsurgical approach is the first choice in the adult population with cartilage damage and knee OA. Yearly, more than 10% of knee OA-affected patients undergo intra-articular injections of different drugs, especially within three months after OA diagnosis. Several molecules, such as corticosteroids injection, hyaluronic acid (HA), and platelet-rich plasma (PRP), are managed to reduce the symptoms of patients with knee OA. The aim of this review was to offer an overview of intra-articular injections used for the treatment of OA and report the conventional pharmacological products used. Full article

A flexible ankle joint is suggested to be a contributing factor for vertical squat jump (SQJ) performance. The purpose of the study was to investigate the effect of the active (ACT) and passive (PAS) ankle joint range of motion (ROM) on SQJ performed by adolescent female volleyball players. ACT and PAS ankle ROM at knee extension angles of 90, 140, and 180 degrees (180 degrees: full extension) were measured with a video analysis method for 35 female post-pubertal volleyball players (16.3 ± 1.1 yrs, 1.80 ± 0.04 m, 68.8 ± 6.8 kg). Additionally, the players fulfilling previously recommended criteria were assigned to the flexible (n = 10) and inflexible (n = 8) groups and executed SQJ with and without an arm swing on a force-plate. Results of the 2 × 2 × 3 MANOVA revealed a significant (p < 0.05) flexibility type and knee angle effect, as ankle ROM was larger in PAS compared to ACT and as the knee joint progressed from 90 to 180 degrees extension. The 2 × 2 ANOVA revealed a significant (p < 0.05) group effect, as flexible players jumped higher in the arm swing SQJ, along with a significant arm swing effect on key SQJ kinetic parameters. In conclusion, a more flexible ankle joint result in improved SQJ performance. Therefore, ankle flexibility training should be implemented in youth volleyball players. Full article

The novel coronavirus disease 2019 (COVID-19) has been responsible for a global pandemic involving massive increases in the daily numbers of cases and deaths. Due to the emergency caused by the pandemic, huge efforts have been made to develop COVID-19 vaccines, the first of which were released in December 2020. Effective vaccines for COVID-19 are needed to protect the population, especially healthcare professionals and fragile individuals, such as older people or chronic-disease-affected patients. Physical exercise training generally has health benefits and assists in the prevention of several chronic diseases. Moreover, physical activity improves mental health by reducing anxiety, depression, and negative mood and improving self-esteem. Therefore, the present review aims to provide a detailed view of the literature, presenting updated evidence on the beneficial effects of adapted physical activity, based on personalized and tailor-made exercise, in preventing, treating, and counteracting the consequences of COVID-19. Full article

This review aimed to synthesize the most up-to-date evidence regarding the prevalence of urinary incontinence (UI) among adolescent female athletes. We conducted a systematic review of studies regarding UI in female athletes less than 19 years of age. This review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRIMSA). The electronic databases of PubMed, Embase, Cochrane Central Register of Controlled Trials (CENTRAL), Scopus, and Web of Science (WOS) were searched between October and November 2020. After blinded peer evaluation, a total of 215 studies were identified and nine were included. Risk of bias was assessed using the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) checklist. This review identified a prevalence of UI in adolescent female athletes between 18% to 80% with an average of 48.58%. The most prevalent sports were trampolining followed by rope skipping. The prevalence of UI among adolescent female athletes practicing impact sports was significantly prevalent. There is a need for further research, education, and targeted interventions for adolescent female athletes with UI. Full article

The aim of this investigation was to identify possible related factors associated to the performance of the crunning test in European children and adolescents. A total number of 559 children and adolescents (age range 6–14 years) of which 308 boys (55.1%) and 251 girls (44.9%), from seven European countries, were screened. A questionnaire concerning demographic and personal life-related factors and a cognitive assessment were performed. A regression analysis was conducted with the performance measures of the crunning movement. T-tests and ANCOVA were used to analyze sub-group differences. Boys have greater crunning performance values compared to girls (5.55 s vs. 7.06 s, p < 0.001) and older children perform better than younger ones (R2 −0.23; p < 0.001). Children with healthy and active habits (exercising or spending time with family members vs. reading or surfing the internet) performed better in the test. Children engaged in team sports had better crunning performances compared to those engaged in individual sports (6.01 s vs. 6.66 s, p = 0.0166). No significant association was found regarding cognitive-related aspects in either children engaged in team or individual sports and the crunning performance. Older and male children performed better in the crunning test than younger and female children. Physical activity-related aspects of children’s life are associated with crunning movement performance. No association was found between higher cognitive performance and the crunning test results. Full article

The objective of this systematic literature review was to evaluate the evidence regarding the development of neck strength in reducing concussion and cervical spine injuries in adult amateur and professional sport populations. PubMed, CINAHL, Science Direct, and Web of Science databases were searched systematically. The criteria for inclusion in the review were as follows: (1) a human adult (≥18 or above); (2) involved in amateur, semi-professional, or professional sports; (3) sports included involved collisions with other humans, apparatus or the environment; (4) interventions included pre- and post-neck muscle strength measures or neck stability measures; (5) outcomes included effects on increasing neck strength in participants and/or injury incidence. Database searches identified 2462 articles. Following title, abstract, and full paper screening, three papers were eligible for inclusion. All of the papers reported information from male participants, two were focused on rugby union, and one on American football. Two of the included studies found a significant improvement in isometric neck strength following intervention. None of the studies reported any impact of neck strengthening exercises on cervical spine injuries. This review has shown that there is currently a lack of evidence to support the use of neck strengthening interventions in reducing impact injury risk in adult populations who participate in sport. Full article

The purpose of this study was to determine the relationships between body composition, strength, power characteristics, sprinting, jumping, and intermittent endurance performance in collegiate male players. Twenty-three players participated (19.7 ± 1.6 yrs; 71.8 ± 7.1 kg; 176.5 ± 5.1 cm). Measurements of interest in body composition included body fat percentage (BF%), lean body mass (LBM), and body mass (BM). Power characteristics were measured with an unloaded squat jump (SJ0) and loaded SJ at 20 kg (SJ20) and 40 kg (SJ40), and unloaded countermovement jump (CMJ0). Power assessments included peak power (PP) and PP allometrically scaled (PPa). Strength characteristics were assessed using isometric mid-thigh pull. Strength assessment included isometric peak force (IPF) and IPF allometrically scaled (IPFa). Performance measures included 10m and 20 m sprint time, CMJ0 jump-height, and Yo-Yo intermittent endurance test level 1 distance. Significant correlations ranging from moderate to very large were found for LBM and CMJ jump height (CM0 JH) (p = 0.01, r = 0.50); BF% and sprint times at 10 m (p = 0.03, r = 0.44) and 20 m (p = 0.02, r = 0.50). PP and PPa from SJ0 and CMJ0 were significantly correlated to 10m sprint time (p < 0.05, r = −0.45 to −0.53) and 20 m sprint time (p < 0.05, r = −0.40 to −0.49). Our findings agree with previous literature in that body composition and power characteristics are directly related to soccer-related performance. Full article

Articular cartilage injury and repair is an issue of growing importance. Although common, defects of articular cartilage present a unique clinical challenge due to its poor self-healing capacity, which is largely due to its avascular nature. There is a critical need to better study and understand cellular healing mechanisms to achieve more effective therapies for cartilage regeneration. This article aims to describe the key features of cartilage which is being modelled using tissue engineered cartilage constructs and ex vivo systems. These models have been used to investigate chondrogenic differentiation and to study the mechanisms of cartilage integration into the surrounding tissue. The review highlights the key regeneration principles of articular cartilage repair in healthy and diseased joints. Using co-culture models and novel bioreactor designs, the basis of regeneration is aligned with recent efforts for optimal therapeutic interventions. Full article

TeamGym is a popular form of gymnastics, including tumbling (Tu), trampette (Tr) and floor exercises (F) characterized by intensive practice placing high levels of stress on athletes. The aim of the study was to investigate athletes’ stress-related changes during TeamGym training and competition, considering hormonal and enzymatic responses (i.e., salivary cortisol and alpha-amylase). Ten (5 males and 5 females) TeamGym athletes (age: 22–28 y) were tested twice at the same time before training and competition; furthermore, for excluding circadian effect on hormonal and enzymatic responses, they were tested at the same time during a rest day. Alpha-amylase and cortisol were measured 15 min before the beginning of exercise, after each gymnastic equipment performance, and after thirty minutes from the end of the performance. Factorial ANOVA with repeated measures was used to verify differences between training and competition (p < 0.05). Competition elicited higher values of alpha-amylase than training (p ranging from 0.001 to 0.019) and rest (p ranging from 0.001 to 0.019). Cortisol showed no exercise induced increase, and its concentrations were higher prior to training compared to competition. TeamGym responses confirm other sports findings in stating that competition elicits higher stress response than training and suggest that salivary alpha-amylase is a more sensitive marker than cortisol to psychophysiological stress also in gymnastics intermittent performance. Full article