Microorganisms

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Special Issue Editor

Special Issue Information

Dear Colleagues,

This Special Issue is the continuation of our previous Special Issue, “Molecular Analysis of Plant Pathogenic Bacteria” (https://0-www-mdpi-com.brum.beds.ac.uk/journal/microorganisms/special_issues/plant_pathogenic_bacteria).

Bacterial taxonomy is currently in a state of flux. As we learn more about bacteria through the application of molecular techniques, the accuracy of taxonomic identification can come into question. What type of analysis will suffice for an identification down to the subspecies level? What techniques are being used to separate species and subspecies within the different plant genera? What are we using to investigate pathogenicity, and is this translating to disease severity in plants? How is the use of molecular techniques helping us to better understand bacterial pathogens? In this Special Issue, we plan to explore the use of molecular techniques to correctly identify bacterial pathogens and pathogenicity.

Dr. Toni A. Chapman
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

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Published Papers (2 papers)

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Research

15 pages, 2310 KiB

Open AccessArticle

Physiological Functions of the Cello-Oligosaccharides Binding CebE in the Pathogenic Streptomyces sp. AMCC400023

by Qiuyue Li, Jiawen Chang, Peiwen Lv, Junxia Li, Yuxia Duan, Dandan Tian, Fei Ge, Gaoya Su, Fengjie Nie, Zheng Gao, Chunyu Yang and Bo Zhou

Microorganisms 2024, 12(3), 499; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms12030499 - 29 Feb 2024

Viewed by 626

Abstract

Potato common scab, an economically important disease worldwide, is caused by pathogenic Streptomyces strains mainly through the effects of thaxtomin. The cello-oligosaccharides binding protein CebE is proposed as a gateway to the pathogenic development of Streptomyces scabiei. In this study, two functional CebE encoding genes, GEO5601 and GEO7671, were identified in pathogenic Streptomyces sp. AMCC400023. With a higher binding affinity towards signal molecules, the deletion of GEO5601 severely impaired thaxtomin-producing capacity and reduced the strain’s pathogenicity. Transcriptional analysis confirmed that CebE⁵⁶⁰¹ is also responsible for the import and provision of carbon sources for cell growth. With lower binding affinity, the pathogenicity island (PAI)-localized CebE⁷⁶⁷¹ may assume a new function of mediating the biological process of sporulation, given the significantly impaired formation of ΔGEO7671 spores. The mechanisms of action of CebE proteins unraveled in Streptomyces sp. AMCC400023 will help pave the way for more effective prevention of the potato common scab disease. Full article

(This article belongs to the Special Issue Molecular Analysis of Plant Pathogenic Bacteria, 2nd Edition)

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17 pages, 2877 KiB

Open AccessArticle

Rapid Detection and Quantification of Viable Cells of Pectobacterium brasiliense Using Propidium Monoazide Combined with Real-Time PCR

by Junhui Li, Ruxing Chen, Ruwei Yang, Xinchen Wei, Hua Xie, Yanxia Shi, Xuewen Xie, Ali Chai, Tengfei Fan, Baoju Li and Lei Li

Microorganisms 2023, 11(11), 2808; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms11112808 - 19 Nov 2023

Viewed by 994

Abstract

Pectobacterium brasiliense (Pbr) has caused significant economic losses in major vegetable production areas in Northern China by causing bacterial soft rot in cash crops such as potatoes and cucumbers. This study aimed to establish a PMA-qPCR detection method for Pbr by screening specific and sensitive primers based on the glu gene and the conserved region of the 23S rRNA gene. Based on the optimized PMA pretreatment conditions, a standard curve was designed and constructed for PMA-qPCR detection (y = −3.391x + 36.28; R² = 0.99). The amplification efficiency reached 97%, and the lowest detection limit of viable cells was approximately 2 × 10² CFU·mL⁻¹. The feasibility of the PMA-qPCR method was confirmed through a manually simulated viable/dead cell assay under various concentrations. The analysis of potato tubers and cucumber seeds revealed that nine naturally collected seed samples contained a range from 10² to 10⁴ CFU·g⁻¹ viable Pbr bacteria. Furthermore, the system effectively identified changes in the number of pathogenic bacteria in cucumber and potato leaves affected by soft rot throughout the disease period. Overall, the detection and prevention of bacterial soft rot caused by Pbr is crucial. Full article

(This article belongs to the Special Issue Molecular Analysis of Plant Pathogenic Bacteria, 2nd Edition)

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