Advances in Molecular Assays for the Detection and Quantification of Pathogens

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Molecular Microbiology and Immunology".

Deadline for manuscript submissions: closed (31 January 2022) | Viewed by 2178

Special Issue Editors


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Guest Editor
Laboratory of Neurobiology and Pathological Physiology, Institute of Animal Physiology and Genetics, CAS, Libechov, Czech Republic
Interests: qPCR; MOL-PCR; dPCR; detection; quantification; mycobacteria

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Guest Editor
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna Bruno Ubertini, Brescia, Italy
Interests: molecular diagnosis; quantification of pathogens; mycobacteria; food pathogens

Special Issue Information

Dear Colleagues,

Molecular diagnostic methods based on the amplification of nucleic acids have now became gold standard methods for the detection of numerous pathogens, including viruses and bacteria but also parasites and other microorganisms. Many of these methods allow not only establishing the presence of the pathogen nucleic acid in the analyzed material but also their enumeration. This quantification becomes essential in data interpretation and can significantly contribute to the development of risk analysis models. The utilization of modern methods that allow precise quantification (e.g., dPCR) or higher levels of multiplexing (e.g., suspension arrays) together with the various modifications of qPCR represent real advances in the detection and quantification of pathogens. Decision making based on quantitative data brings further knowledge toward understanding various aspects of pathogen behavior.

The topic aims at collecting a series of original manuscripts focusing on aspects linked to the detection and quantification of pathogens using molecular methods. New as well as old approaches – though coupled with new rigorous and significant improvements – will be considered for publication.

Dr. Petr Kralik
Dr. Matteo Ricchi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • qPCR
  • dPCR
  • suspension array
  • pathogen
  • viruses
  • parasites
  • multiplex
  • detection
  • quantification

Published Papers (1 paper)

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Research

10 pages, 613 KiB  
Article
Goat Paratuberculosis: Experimental Model for the Evaluation of Mycobacterium Persistence in Raw Milk Cheese
by Giulia Pagliasso, Alessia Di Blasio, Nicoletta Vitale, Angelo Romano, Lucia Decastelli, Antonio Quasso, Matteo Ricchi, Alessandro Dondo, Paolo Pastorino, Maria Silvia Gennero and Stefania Bergagna
Microorganisms 2021, 9(10), 2032; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9102032 - 25 Sep 2021
Cited by 2 | Viewed by 1734
Abstract
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of chronic proliferative enteritis found in ruminants, known as paratuberculosis (PTB). The spread of PTB is increasing in countries with advanced animal husbandry practices, leading to significant economic losses. Moreover, a supposed zoonotic [...] Read more.
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of chronic proliferative enteritis found in ruminants, known as paratuberculosis (PTB). The spread of PTB is increasing in countries with advanced animal husbandry practices, leading to significant economic losses. Moreover, a supposed zoonotic role of MAP in Crohn’s disease (CD) in humans has been discussed by the scientific community; however, although the association between MAP and CD has generally been accepted, it is still up for debate if MAP is the main cause of CD, a contributing factor, or merely a commensal organism for the development of CD. The aim of this study was to assess the survival of MAP during the entire production process of a traditional Italian goat’s raw milk fresh cheese, the “Robiola di Roccaverano”, assessing the survival rate and persistence of MAP in the final product. A mix of MAP field isolates from goats of the Roccaverano area and a reference ATCC strain were used to carry out milk in experimental inoculation. Samples of milk, curd and cheese were taken in two consecutive batches of production. Microbiological challenge tests, evaluated by f57-qPCR, showed a significant decrease in MAP charge during the cheesemaking process for both batches, suggesting the productive process has an impact on MAP survival. Full article
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