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Microorganisms
Special Issues
Gene Analysis in Bacillus subtilis
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Gene Analysis in Bacillus subtilis

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Molecular Microbiology and Immunology".

Deadline for manuscript submissions: closed (30 December 2021) | Viewed by 5898

Special Issue Editors

Dr. Nicolas Mirouze

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Guest Editor
Microbiology department, Institute for Integrative Biology of the Cell (I2BC), Universite Paris Saclay, Paris, France
Interests: gram positive bacteria; environmental adaptation; horizontal gene transfer; competence; natural transformation; gene expression; gene regulation
Dr. Etienne Dervyn

E-Mail
Guest Editor
SyBER Team, Micalis Institut, INRAE, Jouy en Josas France, Paris, France
Interests: Bacillus; gene expression; gene regulation; DNA segregation; synthetic biology; chassis strains

Special Issue Information

Dear Colleagues,

Bacillus subtilis is an important model organism for the study of Gram-positive bacteria thanks to its phylogenetic proximity with important human pathogens, its ability to induce important environmental adaptations, its use in the industry, and the numerous genetic tools available.

This Special Issue offers the opportunity to share recent advances in gene analysis in B. subtilis from chromosome organization, to new genes acquisitions (by horizontal gene transfer, for example), to the expression/regulation/function of genes. This Special Issue will also consider advances in synthetic biology and metabolic engineering in B. subtilis.

Dr. Nicolas Mirouze
Dr. Etienne Dervyn
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Bacillus subtilis
  • chromosome organization
  • horizontal gene transfer
  • expression
  • regulation
  • function

Published Papers (2 papers)

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Research

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17 pages, 3378 KiB  
Article
Assessment of Bacillus subtilis Plasmid pLS20 Conjugation in the Absence of Quorum Sensing Repression
by Kotaro Mori, Valeria Verrone, Ryotaro Amatsu, Kaho Fukui, Wilfried J. J. Meijer, Shu Ishikawa, Anil Wipat and Ken-ichi Yoshida
Microorganisms 2021, 9(9), 1931; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9091931 - 10 Sep 2021
Cited by 4 | Viewed by 2398
Abstract
Bacillus subtilis conjugative plasmid pLS20 uses a quorum-sensing mechanism to control expression levels of its conjugation genes, involving the repressor RcopLS20, the anti-repressor RappLS20, and the signaling peptide Phr*pLS20. In previous studies, artificial overexpression of rappLS20 [...] Read more.
Bacillus subtilis conjugative plasmid pLS20 uses a quorum-sensing mechanism to control expression levels of its conjugation genes, involving the repressor RcopLS20, the anti-repressor RappLS20, and the signaling peptide Phr*pLS20. In previous studies, artificial overexpression of rappLS20 in the donor cells was shown to enhance conjugation efficiency. However, we found that the overexpression of rappLS20 led to various phenotypic traits, including cell aggregation and death, which might have affected the correct determination of the conjugation efficiency when determined by colony formation assay. In the current study, conjugation efficiencies were determined under different conditions using a two-color fluorescence-activated flow cytometry method and measuring a single-round of pLS20-mediated transfer of a mobilizable plasmid. Under standard conditions, the conjugation efficiency obtained by fluorescence-activated flow cytometry was 23-fold higher than that obtained by colony formation. Furthermore, the efficiency difference increased to 45-fold when rappLS20 was overexpressed. Full article
(This article belongs to the Special Issue Gene Analysis in Bacillus subtilis)
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Review

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14 pages, 2009 KiB  
Review
Cis- and Trans-Encoded Small Regulatory RNAs in Bacillus subtilis
by Sabine Brantl and Peter Müller
Microorganisms 2021, 9(9), 1865; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9091865 - 02 Sep 2021
Cited by 12 | Viewed by 2976
Abstract
Small regulatory RNAs (sRNAs) that act by base-pairing are the most abundant posttranscriptional regulators in all three kingdoms of life. Over the past 20 years, a variety of approaches have been employed to discover chromosome-encoded sRNAs in a multitude of bacterial species. However, [...] Read more.
Small regulatory RNAs (sRNAs) that act by base-pairing are the most abundant posttranscriptional regulators in all three kingdoms of life. Over the past 20 years, a variety of approaches have been employed to discover chromosome-encoded sRNAs in a multitude of bacterial species. However, although largely improved bioinformatics tools are available to predict potential targets of base-pairing sRNAs, it is still challenging to confirm these targets experimentally and to elucidate the mechanisms as well as the physiological role of their sRNA-mediated regulation. Here, we provide an overview of currently known cis- and trans-encoded sRNAs from B. subtilis with known targets and defined regulatory mechanisms and on the potential role of RNA chaperones that are or might be required to facilitate sRNA regulation in this important Gram-positive model organism. Full article
(This article belongs to the Special Issue Gene Analysis in Bacillus subtilis)
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