Phytoplasma Diseases of Trees and Shrubs

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Plant Microbe Interactions".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 2129

Special Issue Editor


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Guest Editor
Department of Pharmacy, University of Salerno, I-84084 Fisciano, Italy
Interests: plant pathology; Phytoplasma diseases

Special Issue Information

Dear Colleagues,

Phytoplasmas are obligate, plant-pathogenic microorganisms of the class Mollicutes associated with diseases of more than a thousand plant species worldwide. In diseased plants, phytoplasmas colonize the phloem sieve tube elements and are transmitted from plant to plant by phloem-feeding homopteran insects, mainly leafhoppers and planthoppers, and less frequently psyllids. Phytoplasmas cause a wide range of symptoms that are either specific or largely non-specific symptoms. The latter are most often common in woody plants.

Phytoplasma diseases of trees and shrubs are widespread and of considerable economic and ecological significance. These diseases differ in their geographic distribution, number and size of the various taxonomic groups and subgroups of the associated phytoplasma(s), and insect vector relationships. In several instances, phytoplasma diseases of trees and shrubs escape observation because affected plants show non-specific symptoms only such as yellowing, stunting and/or decline. Also, the phytoplasma titer in diseased plants, especially in those with non-specific symptoms, is often so low that infections can only be detected through highly sensitive nested PCR assays. Latent phytoplasma infections, which are common in some trees and shrubs, can serve as inoculum reservoirs for further spread to susceptible plants.

This Special Issue covers several aspects of the mentioned diseases including (i) molecular and taxonomic identy of the associated phytoplasmas, (ii) phytoplasma-insect vector relationships, (iii) phytoplasma-plant host interactions, (iv) phytoplasma titer and colonization behavior in affected plants, and (vi) disease management and control.

Dr. Carmine Marcone
Guest Editor

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Keywords

  • yellows and decline diseases
  • Candidatus Phytoplasma’ species
  • 16Sr group/subgroups
  • phytoplasma taxonomy
  • symptomatology
  • etiology
  • epidemiology
  • fruit trees
  • forest trees
  • shrubs

Published Papers (2 papers)

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Research

15 pages, 2808 KiB  
Article
Molecular Characterization of ‘Candidatus Phytoplasma prunorum’ in the Czech Republic and Susceptibility of Apricot Rootstocks to the Two Most Abundant Haplotypes
by Tomáš Kiss, Dana Šafářová, Milan Navrátil and Tomáš Nečas
Microorganisms 2024, 12(2), 399; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms12020399 - 17 Feb 2024
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Abstract
Candidatus Phytoplasma prunorum’ is one of the most destructive pathogens of Prunus species, where susceptible species render unproductive several years after infection. In epidemiology, the molecular characterization of phytoplasmas is based on sequence analysis of variable nonribosomal genes. In this study aceF [...] Read more.
Candidatus Phytoplasma prunorum’ is one of the most destructive pathogens of Prunus species, where susceptible species render unproductive several years after infection. In epidemiology, the molecular characterization of phytoplasmas is based on sequence analysis of variable nonribosomal genes. In this study aceF, pnp, imp and secY genes were used for characterization of the ‘Ca. P. prunorum’ genotypes present in the Czech Republic. In total, 56 plant and 33 vector (Cacopsylla pruni) samples positive to ‘Ca. P. prunorum’ collected in seven localities were used in the study. Based on sequence analysis, four aceF, two pnp, six imp, and three secY genotypes were identified in analyzed samples. The most abundant in both plant and insect samples were the A6, P2, I4, and S2 genotypes. Most of the Czech ‘Ca. P. prunorum’ haplotypes clustered together in the haplotype network analysis. Next, two isolates representing the two most abundant Czech haplotypes (A6-P2-I4-S2 and A5-P2-I4-S2) were used in the susceptibility test of three apricot rootstock types (St. Julien A, M-VA-1, GF-305). Susceptibility was analyzed by phytoplasma quantification using quantitative real-time PCR and evaluation of symptom manifestation. Based on the results, the influence of the rootstock type on the phytoplasma titer and symptom manifestation was greater than of the phytoplasma isolate, while the year of analysis had no influence on the results. The results also showed that the phytoplasma titer is increasing in plant tissues during the vegetation period. Full article
(This article belongs to the Special Issue Phytoplasma Diseases of Trees and Shrubs)
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13 pages, 2939 KiB  
Article
Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization
by Franco Daniel Fernández, Xiao-Hua Yan, Chih-Horng Kuo, Carmine Marcone and Luis Rogelio Conci
Microorganisms 2024, 12(1), 142; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms12010142 - 11 Jan 2024
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Abstract
Candidatus Phytoplasma meliae’ is a pathogen associated with chinaberry yellowing disease, which has become a major phytosanitary problem for chinaberry forestry production in Argentina. Despite its economic impact, no genome information of this phytoplasma has been published, which has hindered its characterization [...] Read more.
Candidatus Phytoplasma meliae’ is a pathogen associated with chinaberry yellowing disease, which has become a major phytosanitary problem for chinaberry forestry production in Argentina. Despite its economic impact, no genome information of this phytoplasma has been published, which has hindered its characterization at the genomic level. In this study, we used a metagenomics approach to analyze the draft genome of the ‘Ca. P. meliae’ strain ChTYXIII. The draft assembly consisted of twenty-one contigs with a total length of 751.949 bp, and annotation revealed 669 CDSs, 34 tRNAs, and 1 set of rRNA operons. The metabolic pathways analysis showed that ChTYXIII contains the complete core genes for glycolysis and a functional Sec system for protein translocation. Our phylogenomic analysis based on 133 single-copy genes and genome-to-genome metrics supports the classification as unique ‘Ca. P. species’ within the MPV clade. We also identified 31 putative effectors, including a homolog to SAP11 and others that have only been described in this pathogen. Our ortholog analysis revealed 37 PMU core genes in the genome of ‘Ca. P. meliae’ ChTYXIII, leading to the identification of 2 intact PMUs. Our work provides important genomic information for ‘Ca. P. meliae’ and others phytoplasmas for the 16SrXIII (MPV) group. Full article
(This article belongs to the Special Issue Phytoplasma Diseases of Trees and Shrubs)
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