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Microorganisms
Special Issues
Animal Virology, Molecular Diagnostics and Vaccine Development
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Animal Virology, Molecular Diagnostics and Vaccine Development

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Virology".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 724

Special Issue Editors

Dr. Elizabeth V. Fowler

E-Mail Website
Guest Editor
Queensland Department of Agriculture and Fisheries, Brisbane, QLD, Australia
Interests: point-of-care and molecular diagnostics; virology; vaccine development; genetics
Dr. Rebecca K. Ambrose

E-Mail Website
Guest Editor Assistant
Department of Agriculture and Fisheries, Ecosciences Precinct, Dutton Park, Brisbane, QLD 4102, Australia
Interests: animal biosecurity; virology; genomics; vaccines; diagnostics

Special Issue Information

Dear Colleagues,

Viral infections have a significant impact on animal health worldwide, with major implications for agricultural production systems and biosecurity. Vaccination and diagnostic tools are important for preventing and controlling the spread of diseases among farm animals, which can have significant economic and social impacts. Early detection, management, and control of animal viruses facilitates improvements in the health and well-being of production animals. From a biosecurity perspective, effective vaccination and diagnostic tools will assist with risk mitigation, limit transmission, and identify potential incursions into disease-free countries. The ongoing COVID-19 pandemic has fuelled the development and wide-scale application of novel vaccine technologies and low-cost point-of-care nucleic acid diagnostic platforms for disease pathogens.

This Special Issue of Microorganisms focuses on innovative point-of-care diagnostics tools and modern vaccines: development and applications for animal viruses of agricultural importance. We invite you to submit relevant contributions in the form of original research, systematic reviews, short communications, and other types of articles on related topics.

Dr. Elizabeth V. Fowler
Guest Editor
Dr. Rebecca K. Ambrose
Guest Editor Assistant

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • animal health
  • vaccine
  • in-field diagnostics
  • viral pathogens
  • novel molecular technologies

Published Papers (1 paper)

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Research

11 pages, 2585 KiB  
Article
Establishment of an ELISA Based on a Recombinant Antigenic Protein Containing Multiple Prominent Epitopes for Detection of African Swine Fever Virus Antibodies
by Dossêh Jean Apôtre Afayibo, Zhonghui Zhang, Hualin Sun, Jingsheng Fu, Yaru Zhao, Tharheer Oluwashola Amuda, Mengli Wu, Junzheng Du, Guiquan Guan, Qingli Niu, Jifei Yang and Hong Yin
Microorganisms 2024, 12(5), 943; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms12050943 - 7 May 2024
Viewed by 416
Abstract
African swine fever virus (ASFV) poses a significant threat to the global pig industry, necessitating accurate and efficient diagnostic methods for its infection. Previous studies have often focused on a limited number of epitopes from a few proteins for detecting antibodies against ASFV. [...] Read more.
African swine fever virus (ASFV) poses a significant threat to the global pig industry, necessitating accurate and efficient diagnostic methods for its infection. Previous studies have often focused on a limited number of epitopes from a few proteins for detecting antibodies against ASFV. Therefore, the current study aimed to use multiple B-cell epitopes in developing an indirect Enzyme-Linked Immunosorbent Assay (ELISA) for enhanced detection of ASFV antibodies. For the expression of recombinant protein, k3 derived from 27 multiple peptides of 11 ASFV proteins, such as p72, pA104R, pB602L, p12, p14.5, p49, pE248R, p30, p54, pp62, and pp220, was used. To confirm the expression of the recombinant protein, we used the Western blotting analysis. The purified recombinant K3 protein served as the antigen in our study, and we employed the indirect ELISA technique to detect anti-ASFV antibodies. The present finding showed that there was no cross-reactivity with antibodies targeting Foot-and-mouth disease virus (FMDV), Porcine circovirus type 2 (PCV2), Pseudorabies virus (PRV), Porcine reproductive and respiratory syndrome virus (PRRSV), and Classical swine fever virus (CSFV). Moreover, the current finding was sensitive enough to find anti-ASFV in serum samples that had been diluted up to 32 times. The test (k3-iELISA) showed diagnostic specificity and sensitivity of 98.41% and 97.40%, respectively. Moreover, during the present investigation, we compared the Ingenasa kit and the k3-iELISA to test clinical pig serum, and the results revealed that there was 99.00% agreement between the two tests, showing good detection capability of the k3-iELISA method. Hence, the current finding showed that the ELISA kit we developed can be used for the rapid detection of ASFV antibodies and used as an alternative during serological investigation of ASF in endemic areas. Full article
(This article belongs to the Special Issue Animal Virology, Molecular Diagnostics and Vaccine Development)
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