Advances in Pathogenesis and Genomics of Bacterial Diseases of Catfish

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Bacterial Pathogens".

Deadline for manuscript submissions: closed (31 May 2022) | Viewed by 4690

Special Issue Editors


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Guest Editor
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA
Interests: pathogenesis and genomics of bacterial diseases of catfish; vaccine development

E-Mail Website
Guest Editor
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA
Interests: molecular pathogenesis and identifying virulence factors of bacterial pathogens of catfish; antimicrobial resistant

Special Issue Information

Dear Colleagues

Catfish species represent one of the major aquaculture groups in the United States and several countries across the world including China, Vietnam, Nigeria, and Egypt. The catfish industry has suffered from several bacterial disease outbreaks that cause devastating economic losses. The most prevalent bacterial infections affecting farm-raised catfish are Edwardsiella ictaluri, Flavobacterium species, Aeromonas hydrophila, and Edwardsiella piscicide. The current prevention and control strategies have inherent limitations, leading to production losses. Additionally, knowledge concerning pathogenesis, genomic evolution, and host adaptive strategies of catfish bacterial pathogens are somewhat limited. There is an increasing demand for more studies aiming to investigate the pathogenesis, virulence factors, and genome sequences of catfish bacterial pathogens to make health management decisions and to develop effective preventive strategies.

In this Special Issue, we invite researchers and colleagues to submit research papers and reviews related to different aspects of the bacterial diseases of catfish. Topics of interest include but are not limited to pathogenesis, pathology (histology, immunohistochemistry, and cytochemistry), vaccine development, diagnostic procedures, epidemiology, virulence factor identification, clinical aspects, catfish immune response (including gene/protein expression), prevention, and control. This Issue will also highlight topics related to the bacterial genomics, comparative genomics, molecular genetics, and diversity of catfish bacterial diseases. Furthermore, manuscripts focusing on functional microbiome, microbiome–disease association, and synthetic and alternative treatments are encouraged.

Dr. Mark Lawrence
Dr. Hossam Abdelhamed
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • catfish
  • pathogenesis
  • virulence factors
  • biosecurity
  • reverse genetics
  • metagenomics
  • next-generation sequencing (NGS)
  • RNA-seq
  • bacteria vaccines
  • immunostimulants

Published Papers (2 papers)

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Research

17 pages, 2302 KiB  
Article
Trained Immunity Provides Long-Term Protection against Bacterial Infections in Channel Catfish
by Lora Petrie-Hanson and Ann E. (Beth) Peterman
Pathogens 2022, 11(10), 1140; https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens11101140 - 02 Oct 2022
Cited by 2 | Viewed by 1906
Abstract
Beta glucan exposure induced trained immunity in channel catfish that conferred long-term protection against Edwardsiella ictaluri and Edwardsiella piscicida infections one month post exposure. Flow cytometric analyses demonstrated that isolated macrophages and neutrophils phagocytosed higher amounts of E. ictaluri and E. piscicida. [...] Read more.
Beta glucan exposure induced trained immunity in channel catfish that conferred long-term protection against Edwardsiella ictaluri and Edwardsiella piscicida infections one month post exposure. Flow cytometric analyses demonstrated that isolated macrophages and neutrophils phagocytosed higher amounts of E. ictaluri and E. piscicida. Beta glucan induced changes in the distribution of histone modifications in the monomethylation and trimethylation of H3K4 and modifications in the acetylation and trimethylation of H3K27. KEGG pathway analyses revealed that these modifications affected expressions of genes controlling phagocytosis, phagosome functions and enhanced immune cell signaling. These analyses correlate the histone modifications with gene functions and to the observed enhanced phagocytosis and to the increased survival following bacterial challenge in channel catfish. These data suggest the chromatin reconfiguration that directs trained immunity as demonstrated in mammals also occurs in channel catfish. Understanding the mechanisms underlying trained immunity can help us design prophylactic and non-antibiotic based therapies and develop broad-based vaccines to limit bacterial disease outbreaks in catfish production. Full article
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11 pages, 2431 KiB  
Article
Sanguinarine Protects Channel Catfish against Aeromonas hydrophila Infection by Inhibiting Aerolysin and Biofilm Formation
by Lushan Zhang, Liang Ma, Qiuhong Yang, Yongtao Liu, Xiaohui Ai and Jing Dong
Pathogens 2022, 11(3), 323; https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens11030323 - 07 Mar 2022
Cited by 11 | Viewed by 2286
Abstract
Aeromonas hydrophila is a pathogenic bacterium that can cause serious infections both in humans and aquatic animals. Antibiotics are the main approach for fighting against the pathogen. However, the emergence of antibiotic resistance has resulted in treatment failure. Therefore, drugs with novel strategies [...] Read more.
Aeromonas hydrophila is a pathogenic bacterium that can cause serious infections both in humans and aquatic animals. Antibiotics are the main approach for fighting against the pathogen. However, the emergence of antibiotic resistance has resulted in treatment failure. Therefore, drugs with novel strategies need to be developed. Quorum sensing has been recognized as a promising method for identifying anti-virulence drugs against bacterial infections. The aim of this study was to identify novel drugs targeting quorum sensing of A. hydrophila as alternatives of antibiotics in aquaculture. Thus, hemolytic activity, biofilm formation, qPCR and experimental therapeutics assays were conducted. The results showed that sanguinarine inhibited the growth of A. hydrophila at concentrations higher than 16 μg/mL, but the production of aerolysin and biofilm formation was significantly inhibited at sub-inhibitory concentrations by disrupting the quorum sensing system. Cell viability results showed that sanguinarine could provide protection for A549 cells from aerolysin-induced cell injury. In addition, the mortality of channel catfish administered with sanguinarine at a dosage of 20 mg/kg decreased to 40%, which showed a significant decrease compared with fish in positive group. Taken together, these findings demonstrated that anti-virulence strategies can be a powerful weapon for fighting against bacterial pathogens and sanguinarine appears to be a promising candidate in the treatment of A. hydrophila infections. Full article
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