In Vitro Propagation and Cryopreservation of Plants
A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Physiology and Metabolism".
Deadline for manuscript submissions: 31 July 2024 | Viewed by 2092
Special Issue Editor
Interests: biodiversity conservation; cryopreservation; plant biotechnology
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
Call upon the importance of plant biodiversity for humankind and their threatening level. Cryopreservation, the storing of biological samples in liquid nitrogen (LN), can offer valuable options for non-orthodox seeds, vegetatively propagated species, and cell cultures. In vitro propagation is also helpful for preparing plant materials for cryopreservation, especially threatened wild species. Moreover, as is common sense among cryobiologists, the success of cryopreservation depends on the vigor of plant materials provided by the in vitro culture and the regrowth protocol. In the era of cryobanking germplasm collections of food and agriculture, we still need to develop cryo-biotechnology through principle studies, systematic approaches, and practical applications. Since cryopreservation is a multidisciplinary process, approaches for tuning the whole process or focusing on specific stages, i.e., plant material preparation, pre-LN, cooling/rewarming and unloading, post-LN regrowth, etc., are welcome. This Special Issue of Plants will highlight all aspects of in vitro propagation and cryopreservation technologies to solve plant conservation problems.
Prof. Dr. Haeng-hoon Kim
Guest Editor
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.
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Keywords
- Keywords: Breeding; cryoconservation; Humulus lupulus; pollination; Solanum tuberosum; variety
Planned Papers
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Analysis of thermal characteristics of potato and hop pollen for their cryopreservation and cross-breeding
Miloš Faltus 1*, Jaroslava Domkářová 2, Petr Svoboda 3, Vendulka Horáčková 2, Vladimír Nesvadba 3, Vladislav Klička 4, Alois Bilavčík 2 and Jiří Zámečník 2
Abstract: This study investigated the thermal properties of potato and hop pollen for cryopreservation and following crossbreeding purposes. Using a differential scanning calorimeter, the phase transitions and water content of fresh and dehydrated pollen samples were measured. Potato pollen showed high variability in water content (0.1-0.6 g/g) and crystalline fraction (0-14%), and a wide range of glass transition temperatures (-80 to +40 °C). Hop pollen had lower water content (0.1-0.3 g/g) and no detectable freezing or melting events, with glass transition temperatures between -53 and +15 °C. Both types of pollen maintained high viability before and after cryopreservation, except when exposed to unfavorable conditions. The cryopreserved pollen was successfully used to produce hybrids of potato and hop. The results suggest that cryopreservation is a feasible technique for preserving and utilizing pollen of these crops.