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Advances in Somatic Embryogenesis Plant Cell Differentiation
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Advances in Somatic Embryogenesis Plant Cell Differentiation

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Cell Biology".

Deadline for manuscript submissions: closed (20 December 2023) | Viewed by 5190

Special Issue Editors

Dr. Víctor M. Loyola-Vargas

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Guest Editor
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán CP 97205, Mexico
Interests: somatic embryogenesis; cell differentiation; proteomics; enzyme kinetics
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Neftalí Ochoa-Alejo

E-Mail Website
Guest Editor
Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato 36924, México
Interests: Capsicum; chili pepper; tissue culture; biochemistry; molecular biology; secondary compounds; transcriptomics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The study of somatic embryogenesis (SE) is one of the topics in biology that has most developed in recent years. However, various aspects of this extraordinary differentiation process remain to be understood. There are a wide variety of questions that are still unanswered. Why are there differences in SE induction between monocotyledonous and eudicotyledon plants? What is the signaling mechanism that leads to SE? There are many other questions similar to those above. Additionally, SE has various applications, such as the mass propagation of elite plants, the production of artificial seeds, and its use to study the various aspects of embryo formation. For all these reasons, we call on our colleagues in the scientific community who study SE to publish their research progress in this Special Issue.

Dr. Víctor M. Loyola-Vargas
Prof. Dr. Neftalí Ochoa-Alejo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant cell differentiation
  • somatic embryogenesis
  • plant growth regulators
  • genomics of SE
  • transcriptomics of SE
  • epigenomics of SE
  • proteomics of SE
  • metabolomics of SE

Published Papers (4 papers)

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Research

19 pages, 5445 KiB  
Article
Proteomic Approach during the Induction of Somatic Embryogenesis in Coffea canephora
by Ana Odetth Quintana-Escobar, Esaú Bojórquez-Velázquez, Eliel Ruiz-May and Víctor Manuel Loyola-Vargas
Plants 2023, 12(24), 4095; https://0-doi-org.brum.beds.ac.uk/10.3390/plants12244095 - 07 Dec 2023
Viewed by 936
Abstract
Plant growth regulators (PGR) are essential for somatic embryogenesis (SE) in different species, and Coffea canephora is no exception. In our study model, previously, we have been able to elucidate the participation of various genes involved in SE by using different strategies; however, [...] Read more.
Plant growth regulators (PGR) are essential for somatic embryogenesis (SE) in different species, and Coffea canephora is no exception. In our study model, previously, we have been able to elucidate the participation of various genes involved in SE by using different strategies; however, until now, we have not used a proteomic approach. This research seeks to contribute to understanding the primary cellular pathways involved in developing SE in C. canephora. The process of our model consists of two stages: (1) preconditioning in MS medium with auxin (NAA) and cytokinin (KIN), and (2) induction in Yasuda liquid medium added with cytokinin (BA). Therefore, in this study, we analyzed different days of the SE induction process using shotgun label-free proteomics. An amount of 1630 proteins was found among different sampling days of the process, of which the majority were accumulated during the induction stage. We found that some of the most enriched pathways during this process were the biosynthesis of amino acids and secondary metabolites. Eighteen proteins were found related to auxin homeostasis and two to cytokinin metabolism, such as ABC, BIG, ILR, LOG, and ARR. Ten proteins and transcription factors related to SE were also identified, like SERK1, SKP1, nuclear transcription factor Y, MADS-box, and calreticulin, and 19 related to other processes of plant development, among which the 14-3-3 and PP2A proteins stand out. This is the first report on the proteomic approach to elucidate the mechanisms that operate during the induction of SE in C. canephora. So, our findings provide the groundwork for future, more in-depth research. Data are available via ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD047172. Full article
(This article belongs to the Special Issue Advances in Somatic Embryogenesis Plant Cell Differentiation)
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14 pages, 2411 KiB  
Article
Time-Dependent Proteomic Signatures Associated with Embryogenic Callus Induction in Carica papaya L.
by Lucas Rodrigues Xavier, Caio Cezar Guedes Corrêa, Roberta Pena da Paschoa, Karina da Silva Vieira, Daniel Dastan Rezabala Pacheco, Lucas do Espirito Santo Gomes, Bárbara Cardoso Duncan, Laís dos Santos da Conceição, Vitor Batista Pinto, Claudete Santa-Catarina and Vanildo Silveira
Plants 2023, 12(22), 3891; https://0-doi-org.brum.beds.ac.uk/10.3390/plants12223891 - 18 Nov 2023
Viewed by 1118
Abstract
Sex segregation increases the cost of Carica papaya production through seed-based propagation. Therefore, in vitro techniques are an attractive option for clonal propagation, especially of hermaphroditic plants. Here, we performed a temporal analysis of the proteome of C. papaya calli aiming to identify [...] Read more.
Sex segregation increases the cost of Carica papaya production through seed-based propagation. Therefore, in vitro techniques are an attractive option for clonal propagation, especially of hermaphroditic plants. Here, we performed a temporal analysis of the proteome of C. papaya calli aiming to identify the key players involved in embryogenic callus formation. Mature zygotic embryos used as explants were treated with 20 μM 2,4-dichlorophenoxyacetic acid to induce embryogenic callus. Total proteins were extracted from explants at 0 (zygotic embryo) and after 7, 14, and 21 days of induction. A total of 1407 proteins were identified using a bottom-up proteomic approach. The clustering analysis revealed four distinct patterns of protein accumulation throughout callus induction. Proteins related to seed maturation and storage are abundant in the explant before induction, decreasing as callus formation progresses. Carbohydrate and amino acid metabolisms, aerobic respiration, and protein catabolic processes were enriched throughout days of callus induction. Protein kinases associated with auxin responses, such as SKP1-like proteins 1B, accumulated in response to callus induction. Additionally, regulatory proteins, including histone deacetylase (HD2C) and argonaute 1 (AGO1), were more abundant at 7 days, suggesting their role in the acquisition of embryogenic competence. Predicted protein–protein networks revealed the regulatory role of proteins 14-3-3 accumulated during callus induction and the association of proteins involved in oxidative phosphorylation and hormone response. Our findings emphasize the modulation of the proteome during embryogenic callus initiation and identify regulatory proteins that might be involved in the activation of this process. Full article
(This article belongs to the Special Issue Advances in Somatic Embryogenesis Plant Cell Differentiation)
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16 pages, 2956 KiB  
Article
Callus Type, Growth Regulators, and Phytagel on Indirect Somatic Embryogenesis of Coffee (Coffea arabica L. var. Colombia)
by Consuelo Margarita Avila-Victor, Enrique de Jesús Arjona-Suárez, Leobardo Iracheta-Donjuan, Jorge Manuel Valdez-Carrasco, Fernando Carlos Gómez-Merino and Alejandrina Robledo-Paz
Plants 2023, 12(20), 3570; https://0-doi-org.brum.beds.ac.uk/10.3390/plants12203570 - 14 Oct 2023
Viewed by 1372
Abstract
Coffee is a crop of global relevance. Indirect somatic embryogenesis has allowed plants of different coffee genotypes to be massively regenerated. The culture medium composition can affect the calli characteristics that are generated and their ability to form somatic embryos. This research aimed [...] Read more.
Coffee is a crop of global relevance. Indirect somatic embryogenesis has allowed plants of different coffee genotypes to be massively regenerated. The culture medium composition can affect the calli characteristics that are generated and their ability to form somatic embryos. This research aimed to determine the influence of the type of callus, growth regulators, and phytagel concentration on the embryogenic capacity of the Colombia variety. Leaf explants were cultured on Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5–1.0 mg L−1), benzylaminopurine (BAP, 1.0 mg L−1), and phytagel (2.3–5.0 g L−1). The explants generated two types of calli: friable (beige, soft, watery, easy disintegration, polyhedral parenchyma cells) and compact (white, hard, low water content, difficult disintegration, elongated parenchyma cells). About 68% of the total callus generated was compact; this type of callus produced a greater number of embryos (71.3) than the friable one (29.2). The number of differentiated embryos was significantly affected by the concentration of phytagel; higher concentrations (5.0 g L−1) resulted in larger quantities (73.7). The highest number of embryos (127.47) was obtained by combining 1.0 mg L−1 2,4-D, 1.0 mg L−1 BAP, 5.0 g L−1 phytagel, and compact callus. Full article
(This article belongs to the Special Issue Advances in Somatic Embryogenesis Plant Cell Differentiation)
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20 pages, 6295 KiB  
Article
Characterization of the PIN Auxin Efflux Carrier Gene Family and Its Expression during Zygotic Embryogenesis in Persea americana
by Zurisadai Monroy-González, Miguel A. Uc-Chuc, Ana O. Quintana-Escobar, Fátima Duarte-Aké and Víctor M. Loyola-Vargas
Plants 2023, 12(12), 2280; https://0-doi-org.brum.beds.ac.uk/10.3390/plants12122280 - 12 Jun 2023
Viewed by 1220
Abstract
Auxins are responsible for a large part of the plant development process. To exert their action, they must move throughout the plant and from cell to cell, which is why plants have developed complex transport systems for indole-3-acetic acid (IAA). These transporters involve [...] Read more.
Auxins are responsible for a large part of the plant development process. To exert their action, they must move throughout the plant and from cell to cell, which is why plants have developed complex transport systems for indole-3-acetic acid (IAA). These transporters involve proteins that transport IAA into cells, transporters that move IAA to or from different organelles, mainly the endoplasmic reticulum, and transporters that move IAA out of the cell. This research determined that Persea americana has 12 PIN transporters in its genome. The twelve transporters are expressed during different stages of development in P. americana zygotic embryos. Using different bioinformatics tools, we determined the type of transporter of each of the P. americana PIN proteins and their structure and possible location in the cell. We also predict the potential phosphorylation sites for each of the twelve-PIN proteins. The data show the presence of highly conserved sites for phosphorylation and those sites involved in the interaction with the IAA. Full article
(This article belongs to the Special Issue Advances in Somatic Embryogenesis Plant Cell Differentiation)
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