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Separations
Special Issues
Chromatography for the Separation and Detection of Metabolites
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Chromatography for the Separation and Detection of Metabolites

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Chromatographic Separations".

Deadline for manuscript submissions: 20 September 2024 | Viewed by 429

Special Issue Editors

Dr. Emanuela Salviati

E-Mail Website
Guest Editor
Dipartimento di Farmacia/DIFARMA, Università di Salerno, Salerno, Italy
Interests: chromatography; mass spectrometry; hyphenated analytical techniques metabolomics; lipidomics; spatial-omics; MS-imaging; pharmacokinetics
Special Issues, Collections and Topics in MDPI journals
Dr. Manuela Giovanna Basilicata

E-Mail Website
Guest Editor
Department of Advanced Medical and Surgical Sciences, University of Campania “Luigi Vanvitelli”, Naples, Italy
Interests: chromatography; mass spectrometry; hyphenated analytical techniques; peptidomics; natural compounds; pharmacokinetics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Innovative analytical techniques are under constant development in the so-called OMICS research field, including metabolomics, lipidomics, volatilomics and proteomics, as well as in nutraceuticals and pharmaceutical studies.

In particular, it is of utmost importance to develop and optimize separation techniques, mainly based on the hyphenation of chromatography and mass spectrometry, with high selectivity, speed and coverage, for the identification and quantification of metabolites, lipids and proteins in biospecimens, for the characterization of natural products, and for the evaluation of pharmacokinetic and pharmacological properties of bioactive molecules.

Therefore, it is my pleasure to invite you to contribute a research article, communication, or review to this Special Issue dedicated to the development, validation, and application of original separation methods aimed at the qualitative–quantitative analysis of metabolites in the field of omics sciences, nutraceuticals, and pharmaceutical applications.

Dr. Emanuela Salviati
Dr. Manuela Giovanna Basilicata
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chromatography (LC, GC)
  • mass spectrometry
  • hyphenated techniques
  • LC-MS
  • GC-MS
  • metabolomics
  • lipidomics
  • natural products
  • chemical finger-printing
  • pharmaceutical applications

Published Papers (1 paper)

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Research

9 pages, 962 KiB  
Article
Two-Dimensional Thin-Layer Chromatography as an Accessible, Low-Cost Tool for Lipid-Class Profile Screening
by Zipora Tietel
Separations 2024, 11(6), 161; https://0-doi-org.brum.beds.ac.uk/10.3390/separations11060161 - 23 May 2024
Viewed by 316
Abstract
The interest in lipid composition profiling is significantly increasing as research reveals the immense importance of lipids in medicine, plant science, food and agriculture. However, lipidomic analysis requires high-end specialty equipment. We used two-dimensional thin-layer chromatography (2D-TLC) as a readily available, low-cost tool [...] Read more.
The interest in lipid composition profiling is significantly increasing as research reveals the immense importance of lipids in medicine, plant science, food and agriculture. However, lipidomic analysis requires high-end specialty equipment. We used two-dimensional thin-layer chromatography (2D-TLC) as a readily available, low-cost tool for basic lipidomic profiling of lipid classes in algal samples in the models Chlamydomonas reinhardtii, Auxenochlorella protothecoides, and Euglena gracilis, validating lipid class identification using an LC-MS/MS analysis. Algal lipid extracts were separated on a 2D-TLC plate, and TLC analysis was followed by scraping individual TLC spots off the plate, and a subsequent liquid chromatography separation and tandem mass spectrometry (LC-MS/MS) analysis. For comparison, crude lipid extracts were also injected directly to the LC-MS/MS system. Lipid class annotation was achieved by a combination of accurate mass, retention time information, neutral loss and fragment ion analysis by MS2Analyzer, and by matching spectra to LipidBlast MS/MS library. Overall, we were able to identify 15 lipid classes, and to adequately profile the lipid classes in all three organisms. This TLC method is thus suggested as an accessible tool for lipid class profiling of algal, plant, and food lipids, alike, when a rapid and simple analysis is required, e.g., for screening purposes. Full article
(This article belongs to the Special Issue Chromatography for the Separation and Detection of Metabolites)
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