Dear Colleagues,

Analysis of proteins in biological samples is an increasingly evolving and desirable method for studying organisms, their functions, and development. The development of these analyses is made possible by the development of advanced analytical methods. It mainly includes the use of modern methods of mass spectrometry in conjunction with separation techniques. An integral part of this research is the use of appropriate methods for sample preparation that allow targeted analysis (e.g., biomarkers). Thus, the methods may include both targeted analysis and screening studies. It should be borne in mind that proteins are a remarkably diverse group of substances that can differ significantly in their properties. Therefore, the problem of protein analysis in biological samples is a kind of art—analytical art.

The presented Special Issue should include both well-arranged articles providing an overview of the current development of methods and procedures of analyses in real biological samples, as well as experimental works dealing with new procedures and approaches applied in specific analytical problems. Papers on new techniques suitable for protein analysis are also welcome. Of course, the scope of this volume is not limited to the analysis of “pure” proteins but also includes their modifications, such as glycosylation, glycation, phosphorylation, and many others.

The aim of this Special Issue should be to provide a general overview of modern methods used to analyze proteins in biological samples, but also to outline the current trends in these methods, to acquaint the scientific community with modern procedures and approaches to real proteomic analysis, whether targeted or screening studies.

Prof. Dr. Ivan Mikšík
Guest Editor

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• protein analysis
• sample preparation
• target analysis
• glycoprotein
• peptide analysis
• mass spectrometry
• biological sample
• chromatography