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Veterinary Sciences
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Latest Advances in Basic Reproductive Research in Domestic Animals
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Latest Advances in Basic Reproductive Research in Domestic Animals

A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Reproduction and Obstetrics".

Deadline for manuscript submissions: closed (15 June 2022) | Viewed by 6085

Special Issue Editor

Prof. Dr. Fred Sinowatz

E-Mail Website
Guest Editor
Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig Maximilian University, Veterinärstr. 13, D 80539 Munich, Germany
Interests: embryogenesis; ultrastructure; histochemistry; fertilization; angiogenesis; reproductive techniques
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Basic research has in recent years significantly increased our knowledge of the fundamentals of reproduction biology and medicine, which in turn facilitate the development of state-of-the-art management strategies to optimize reproductive efficiency in domestic animals. The aim of this Special Issue is to disseminate the results of high-quality basic studies in several important areas of veterinary reproduction. Topics that will be covered include new results of epigenetics and gamete physiology, sperm sexing for in vitro production of embryos, as well as important new data on the physiology of early pregnancy and placentation. Both original papers as well as timely scientific reviews of the literature on these important topics of reproduction are welcome.

Reproduction in domestic animals is an exciting area of research. The associated events usually reoccur on a scheduled and predictable time frame, such as gametogenesis, estrus cycle, pregnancy, and fetal development. In veterinary and human medicine, a detailed knowledge of the anatomy and physiology of reproductive organs and their cells are a precondition to successfully manipulating the reproductive system in many modern reproductive technologies. This Special Issue of Veterinary Sciences focuses on several important areas of basic reproduction science in domestic animals, such as the epigenetics of gametes, changes in the corpus luteum during the estrus cycle, the interactions of embryo and fetus during early pregnancy, and new results on placentation, where interesting new data have been obtained during the last few years.

A number of well-known scientists in this area have expressed their interest in participating in this project. We are pleased to invite you to contribute to this Special Issue of Veterinary Sciences.

Veterinary Sciences (ISSN 2306-7381) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of veterinary sciences, including basic research on prevention, diagnosis, and treatment of disease of domestic animal. There is no restriction on the length of the papers. This Special Issue of Veterinary Sciences will focus on some important areas of basic reproduction research, especially gamete structure and physiology, regulation and changes in the corpus luteum through the estrous cycle, and the interactions of embryo and fetus during early pregnancy and placentation.

In this Special Issue, original research articles and reviews are welcome. Research areas may include (but are not limited to) the following: epigenetics of germ cells, regulation of corpus luteum development and regression, stem cells, early pregnancy, and placenta.

We look forward to receiving your contributions.

Dr. Fred Sinowatz
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Veterinary Sciences is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • spermatozoa
  • epigenetics
  • sex sorting of spermatozoa
  • corpus luteum
  • prostaglandins
  • stem cells
  • yolk sac
  • early pregnancy
  • placenta

Published Papers (3 papers)

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Research

9 pages, 251 KiB  
Article
Assessment of Semen Respiratory Activity of Domesticated Species before and after Cryopreservation: Boars, Bulls, Stallions, Reindeers and Roosters
by Elena Nikitkina, Ismail Shapiev, Artem Musidray, Anna Krutikova, Kirill Plemyashov, Sofia Bogdanova, Victoria Leibova, Gennadiy Shiryaev and Julia Turlova
Vet. Sci. 2022, 9(10), 513; https://0-doi-org.brum.beds.ac.uk/10.3390/vetsci9100513 - 21 Sep 2022
Cited by 1 | Viewed by 1362
Abstract
To assess sperm quality, it is important to evaluate energy metabolism. The test substance 2.4-dinitrophenol (2.4-DNP) is an agent for destroying oxidative phosphorylation. 2.4-DNP shuts off the production of adenosine triphosphate (ATP) from oxidation and then, the respiration rate increases. If the respiratory [...] Read more.
To assess sperm quality, it is important to evaluate energy metabolism. The test substance 2.4-dinitrophenol (2.4-DNP) is an agent for destroying oxidative phosphorylation. 2.4-DNP shuts off the production of adenosine triphosphate (ATP) from oxidation and then, the respiration rate increases. If the respiratory chain is damaged, there is little or no response to adding 2.4-DNP. The aim of this study was to analyze the respiratory activity and oxidative phosphorylation in semen before and after freezing and compare the obtained data with the fertilizing ability of sperm. There was a reduction in sperm respiration rates in all species after thawing. The respiration of spermatozoa of boars, bulls, stallions, reindeers and chicken showed responses to 2.4-dinitrophenol. The only difference is in the strength of the response to the test substance. After freezing and thawing, respiratory stimulation by 2.4-DNP decreased. The results of our study show that respiration rate is not correlated with pregnancy rates and egg fertility. However, there was a high correlation between the stimulation of respiration by 2.4-dinitrophenol and pregnancy rates. The test for an increase in respiration rate after adding 2.4-dinitrophenol could be a suitable test of the fertilizing ability of sperm. Full article
(This article belongs to the Special Issue Latest Advances in Basic Reproductive Research in Domestic Animals)
20 pages, 1255 KiB  
Article
Effects of Intrauterine Infusion of Micronised Purified Flavonoid Fraction (MPFF) in Metritis-Diagnosed Dairy Cows Naturally Infected by E. coli during the Early Postpartum
by Miguel A. Gutiérrez-Reinoso, José B. Uquilla, Francisco A. Barona, Manuel E. Guano, Gloria N. Chicaiza and Manuel García-Herreros
Vet. Sci. 2022, 9(7), 362; https://0-doi-org.brum.beds.ac.uk/10.3390/vetsci9070362 - 16 Jul 2022
Cited by 2 | Viewed by 2267
Abstract
The occurrence of metritis during the postpartum period causes serious economic losses in dairy cattle. The Micronised Purified Flavonoid Fraction (MPFF) is a polyphenolic flavonoid compound which is considered to have many health-related properties such as antibiotic, anti-inflammatory, phlebotonic, and several vascular-protecting activities. [...] Read more.
The occurrence of metritis during the postpartum period causes serious economic losses in dairy cattle. The Micronised Purified Flavonoid Fraction (MPFF) is a polyphenolic flavonoid compound which is considered to have many health-related properties such as antibiotic, anti-inflammatory, phlebotonic, and several vascular-protecting activities. The aim was to evaluate the effects of a new strategic therapy for metritis based on MPFF intrauterine infusions during the early postpartum in dairy cows naturally infected by Escherichia coli. The clinical effects on reproductive anatomical structures and chronological involution dynamics were monitored until day 24 postpartum by ultrasonography. Moreover, uterine bacteriological and cytological (polymorphonuclear neutrophils; PMNs) profiles were analysed before and after MPFF infusion. The results showed that the success rate (% cure) at day 24 postpartum was improved significantly when using higher MPFF doses (p < 0.05). Moreover, MPFF treatment acutely diminished the size of the cervix and uterus and improved the involution process during the first 24 days (p < 0.05). The prevalence of pathogenic bacteria found in in vitro cultures was significantly variable (p < 0.01), as were the antibiotic sensitivity patterns. Pathogenic bacteria isolates decreased after MPFF applications in a dose–response fashion (p < 0.01), while isolates obtained from controls and low-dose-MPFF-treated animals were stable and similar (p > 0.05). The sensitivity patterns of pathogenic bacteria isolated in in vitro cultures from MPFF-treated animals were variable, although resistance to E. coli, Staphylococcus aureus, Bacillus spp., and coliforms was shown irrespective of the MPFF doses used. However, MPFF-treated cows showed a dose–response effect regarding PMN rates (p < 0.05). The calving-first service, calving–conception interval, and conception rate improved significantly from using higher MPFF doses (p < 0.05). In conclusion, this study shows that MPFF treatment differentially affects uterine involution, bacteriological profiles, cytological traits, and reproductive performance in metritis-positive dairy cows naturally infected by E. coli. Full article
(This article belongs to the Special Issue Latest Advances in Basic Reproductive Research in Domestic Animals)
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10 pages, 904 KiB  
Article
Expression of Antimicrobial Peptide Genes in the Canine Amniotic Membrane
by Rajit Lohajaroensub, Chenphop Sawangmake, Channarong Rodkhum and Nalinee Tuntivanich
Vet. Sci. 2022, 9(5), 200; https://0-doi-org.brum.beds.ac.uk/10.3390/vetsci9050200 - 21 Apr 2022
Cited by 3 | Viewed by 2012
Abstract
The human amniotic membrane has been successfully used in human ocular reconstruction. Several studies have demonstrated its properties, including antimicrobial features. As a result of the restricted availability of human amniotic membrane for veterinary use, canine amniotic membrane has become an attractive alternative. [...] Read more.
The human amniotic membrane has been successfully used in human ocular reconstruction. Several studies have demonstrated its properties, including antimicrobial features. As a result of the restricted availability of human amniotic membrane for veterinary use, canine amniotic membrane has become an attractive alternative. Clinical studies of the application of canine amniotic membrane in animals and the understanding of its biological properties are limited. This study aimed to determine the expression of peptide genes of natural antimicrobials in canine amniotic membrane. Expressions of canine β-defensin 1, 102, and 103, and canine Elafin were determined in healthy puppies by real-time quantitative polymerase chain reaction. Canine β-defensin 1, 103, and Elafin were expressed in all samples, possibly suggesting a role in the innate immune system of normal canine amniotic membrane. Further investigations of protein expression and localization are recommended. Full article
(This article belongs to the Special Issue Latest Advances in Basic Reproductive Research in Domestic Animals)
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