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Method Development for Selected Bisphenols Analysis in Sweetened Condensed Milk from a Can and Breast Milk Samples by HPLC–DAD and HPLC-QqQ-MS: Comparison of Sorbents (Z-SEP, Z-SEP Plus, PSA, C18, Chitin and EMR-Lipid) for Clean-Up of QuEChERS Extract
Article

A New LC-MS/MS Method for Simultaneous and Quantitative Detection of Bisphenol-A and Steroids in Target Tissues: A Power Tool to Characterize the Interference of Bisphenol-A Exposure on Steroid Levels

Department of Experimental Medicine, University of Campania “L. Vanvitelli”,Via S. M. di Costantinopoli, 16, 80138 Naples, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Tomasz Tuzimski
Received: 11 October 2019 / Revised: 12 December 2019 / Accepted: 17 December 2019 / Published: 21 December 2019
Bisphenol A (BPA), an endocrine disruptor, may affect in situ steroidogenesis and alter steroids levels. The present work proposes a liquid chromatography tandem mass spectrometry method to simultaneously quantify BPA, 17β-Estradiol and testosterone in two target tissues: testis and visceral fat mass. Analytes were isolated and lipophilic impurities removed by two serial steps: liquid-liquid and solid phase extraction. All compounds were separated in a single gradient run by Kinetex F5 column and detected via multiple reaction monitoring using a triple quadrupole with a TurboIon electrospray source in both negative and positive modes. The method is selective and very sensitive. In the investigated concentration range, the linearity of the detector response is verified in both tissues. The use of specific SPE cartridges for affinity chromatography purification allows obtaining high percentages of process efficiency (68.0–83.3% for testicular tissue; 63.7–70.7% for visceral fat mass). Good repeatability and reproducibility was observed. The validated method can be efficiently applied for direct biological monitoring in testis and visceral fat mass from mice exposed to BPA. The quantification of compounds in a single assay could be achieved without a loss of sensitivity. View Full-Text
Keywords: Bisphenol A; 17β-estradiol; testosterone; LC-MS/MS; testis; visceral fat Bisphenol A; 17β-estradiol; testosterone; LC-MS/MS; testis; visceral fat
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MDPI and ACS Style

Errico, S.; Chioccarelli, T.; Moggio, M.; Diano, N.; Cobellis, G. A New LC-MS/MS Method for Simultaneous and Quantitative Detection of Bisphenol-A and Steroids in Target Tissues: A Power Tool to Characterize the Interference of Bisphenol-A Exposure on Steroid Levels. Molecules 2020, 25, 48. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules25010048

AMA Style

Errico S, Chioccarelli T, Moggio M, Diano N, Cobellis G. A New LC-MS/MS Method for Simultaneous and Quantitative Detection of Bisphenol-A and Steroids in Target Tissues: A Power Tool to Characterize the Interference of Bisphenol-A Exposure on Steroid Levels. Molecules. 2020; 25(1):48. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules25010048

Chicago/Turabian Style

Errico, Sonia, Teresa Chioccarelli, Martina Moggio, Nadia Diano, and Gilda Cobellis. 2020. "A New LC-MS/MS Method for Simultaneous and Quantitative Detection of Bisphenol-A and Steroids in Target Tissues: A Power Tool to Characterize the Interference of Bisphenol-A Exposure on Steroid Levels" Molecules 25, no. 1: 48. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules25010048

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